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pJET1.2_Nkx3-2_Sense
Plasmid#124437PurposePlasmid for sense in situ probe in vitro transcriptionDepositorInsertNK3 homeobox 2 (Nkx3-2 Mouse)
UseIn situ probeTagsExpressionMutationPromoterT7Available sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2_Rab28_Sense
Plasmid#124441PurposePlasmid for sense in situ probe in vitro transcriptionDepositorInsertRAB28, member RAS oncogene family (Rab28 Mouse)
UseIn situ probeTagsExpressionMutationPromoterT7Available sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJET1.2_Rab28_Anti-Sense
Plasmid#124442PurposePlasmid for anti-sense in situ probe in vitro transcriptionDepositorInsertRAB28, member RAS oncogene family (Rab28 Mouse)
UseIn situ probeTagsExpressionMutationPromoterT7Available sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)
Plasmid#107739Purposemutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein(C1A)
UseTagsExpressionWormMutationchange the first cysteine of gp41-1 N-intein to a…PromoterC. elegans myo-2 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-shScn4b-268
Plasmid#99031PurposeExpresses a shRNA targeting the mouse Scn4b geneDepositorInsertScn4b shRNA (Scn4b Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromotermouse U6Available sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS1412
Plasmid#63787PurposeVector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection markerDepositorInserteCitrine-Strep-tag II followed by URA3 selection marker
UseE. coli cloning vectorTagseCitrine-Strep-tag IIExpressionMutationPromoternoneAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(C631S, C643S and C699T)
Plasmid#52128Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(M1-5) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationC631S, C643S and C699TPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 T7 Jip1b(471-707)
Plasmid#52428Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(471-707) ) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-470PromoterAvailable sinceApril 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(282-707)
Plasmid#52126Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(282-707)) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-281PromoterAvailable sinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBG MKK3b (Glu)
Plasmid#50451Purposeactivates p38 in the signaling pathwayDepositorInsertMKK3b (MAP2K3 Human)
UseTagsGstExpressionMammalianMutationchanged Serine 218 to Glutamic Acid, changed Thre…PromoterSV40Available sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-C6orf60
Plasmid#34813DepositorInsertC6orf60 (FAM184A Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
HOXB6-FLAGA-pSP65
Plasmid#20994DepositorInsertHOXB6 (HOXB6 Human)
UseIn vitro trans/transTagsFLAGExpressionMutationPromoterAvailable sinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C2-MVP
Plasmid#204543PurposeExpresses EGFP-tagged MVP in mammalian cellsDepositorInsertMVP (MVP Human)
UseTagsExpressionMammalianMutationNonePromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-MAP1LC3B
Plasmid#137757PurposeExpression of LC3BDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCWori cytochrome P450-BM3h 9D7
Plasmid#61308PurposeExpresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tagDepositorInsertCytochrome P450-BM3 heme domain 9D7
UseTags6xHisExpressionBacterialMutationT268A, I263A, T438V, A328GPromoterTacAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-GABARAPL1
Plasmid#137759PurposeExpression of GABARAPL1DepositorInsertGABARAPL1 (GABARAPL1 Human)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-GABARAPL2
Plasmid#137761PurposeExpression of GABARAPL2DepositorInsertGABARAPL2 (GABARAPL2 Human)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
HA-MAP1LC3A
Plasmid#137756PurposeExpression of LC3ADepositorInsertMAP1LC3A (MAP1LC3A Human)
UseTagsHA and mycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hADM-RFP
Plasmid#22922DepositorInserthuman adrenomedullin promoter driving DsRedExpress (ADM Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRDB_053
Plasmid#216083Purposefor transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vectorDepositorHas ServiceCloning Grade DNAInsertgypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
UseDestinationTagsExpressionInsectMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNeae2
Plasmid#168300PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli Intimin N-terminal fragment, E-tag, 6xHis tag, and myc-tag at the C-terminus
UseTags6xHis, E-tag, and myc-tagExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-CAG-GFP
Plasmid#83279Purposerecombinant AAV vector packaging self-complementary GFP under the CAG promoterDepositorInsertenhanced green fluorescent protein
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCryptDel4.8
Plasmid#141293PurposePlasmid for curing pMUT2 in one step based on pFREE. Contains RelB antitoxin, as well as gRNA targetting pMUT2 plasmid as well as pCryptDel4.8 itself.DepositorInsertsRelB
gRNA targetting pMUT2
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterNative promoter from pMUT2 relB/relE operon and P…Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
p6XHis_NLS-SaCas9
Plasmid#101086PurposeExpress SaCas9 in bacteria with a 6xHis tag for purificationDepositorInsertCRISPR-associated protein Cas9/Csn1
UseCRISPRTagsTrxA-6xHis-STagExpressionBacterialMutationPromoterT7lacAvailable sinceDec. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMaRSC
Plasmid#89189PurposeThe pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.DepositorInsertFlag-L274G-T2A-mCherry (Mars1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-mKeima-LGALS3
Plasmid#175780PurposeLentiviral vector expressing N-terminally tagged mKeima-LGALS3 (Lyso-Keima), for quantitative assessment of lysophagic fluxDepositorInsertLGALS3 (LGALS3 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX304-IRES-Td
Plasmid#176296PurposepLX304 backbone with an IRES-Tdtomato cassette subcloned into the NheI-AgeI sitesDepositorTypeEmpty backboneUseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only
