We narrowed to 12,307 results for: BASE

1. VV201: ChR2(C128S)-eGFP in fubi

   Plasmid

   #58510

   Purpose

   Expresses channelrhodopsin-2 with the C128S (step function opsin) mutation fused to eGFP under the ubiquitin promoter

   Depositor

   Adam Cohen

   Article

   Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

   Insert

   ChR2(C128S)-eGFP
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Cysteine 128 to Serine

   Promoter

   Ubiquitin

   Available since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR2-R2-PBR-L4

   Plasmid

   #141017

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pENTR2-L3-PBL-R1

   Plasmid

   #141016

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPpT4- TEF2prom-TIR1F74G-FLAG

   Plasmid

   #189727

   Purpose

   Enables expression of OsTIR1(F74G) from the TEF2 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB219)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   TEF2

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPpT4- HTA1prom-TIR1 F74G -FLAG

   Plasmid

   #189728

   Purpose

   Enables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Use

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Promoter

   HTA1

   Available since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCO487

   Plasmid

   #139730

   Purpose

   Module plasmid, individual component of Fungal Bioluminescent Pathway, H3H

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pMOD_B_AtUbi10:NnH3H:tAtuOcs
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CmYLCV

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TALED_Left-stuffer-8e-E1347A

   Plasmid

   #187163

   Purpose

   original vector to make all constructs

   Depositor

   Jin-Soo Kim

   Article

   Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

   Insert

   Left-stuffer-8e-E1347A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCR-Blunt_hNANOG

   Plasmid

   #112639

   Purpose

   Plasmid for PCR amplification of hNANOG DNA template for in vitro transcription

   Depositor

   Ganna Bilousova

   Article

   McGrath et al J Vis Exp. 2018 Nov 26;(141). doi: 10.37

   Insert

   hNANOG (NANOG Human)
   

   Use

   Pcr template

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDONR-P2rP4

   Plasmid

   #141015

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMC-Ura-Cen-lvl1

    Plasmid

    #176182

    Purpose

    preassembled yeast MoClo level-1 backbone with GFP dropout and URA3 marker

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMC-X2

    Plasmid

    #176152

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMC-X3

    Plasmid

    #176153

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-3

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMC-XI1

    Plasmid

    #176155

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-1

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMC-XI5

    Plasmid

    #176158

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-5

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMC-XII2

    Plasmid

    #176160

    Purpose

    preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-2

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCO488

    Plasmid

    #139731

    Purpose

    Module plasmid, individual component of Fungal Bioluminescent Pathway, Hisps

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pMOD_C'_CmYLCV:NnHisps:tHsp
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CmYLCV

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. UBQ10:sXVE:S10-(MCS)

    Plasmid

    #108177

    Purpose

    Binary vectors used for the inducible expression of tripartite split-sfGFP components in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    UBQ10:sXVE:

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC18-iSpinach

    Plasmid

    #186053

    Purpose

    template for co-transcriptional RNA folding assays

    Depositor

    Markus Wahl

    Article

    Huang et al Mol Cell. 2020 Sep 17;79(6):1024-1036.e5

    Insert

    T7A1-rrnG-iSpinach
    

    Use

    Tags

    no

    Expression

    Bacterial

    Mutation

    Promoter

    T7A1

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a-cMSPdH4-6

    Plasmid

    #199766

    Purpose

    Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

    Depositor

    Franz Hagn

    Article

    Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

    Insert

    cMSPdH46
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    internal His-Tag

    Promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pL8_3xG34-DCAF8L2_X1

    Plasmid

    #191519

    Purpose

    Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAC, Right)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    ZF-DdCBE 3xG34-DCAF8L2 X1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pL5_3xG22-DCAF8L2_X1

    Plasmid

    #191516

    Purpose

    Expression of nuclear v8 ZF-DdCBE in mammalian cells (C-terminal DddAN, Left)

    Depositor

    David Liu

    Article

    Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

    Insert

    ZF-DdCBE 3xG22-DCAF8L2 X1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p1.2-Zeo

    Plasmid

    #162740

    Purpose

    Protein expression in CHO cells

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CHO Elongation factor 1A

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV-Tag/WT SPAST with 5'UTR

    Plasmid

    #87731

    Purpose

    Expresses WT spastin M1 and M87 in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TALED_Left-stuffer-1397C-8e

    Plasmid

    #187169

    Purpose

    original vector to make all constructs

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    Left-stuffer-1397C-8e
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TALED_Left-stuffer-1397C-UGI

    Plasmid

    #187168

    Purpose

    original vector to make all constructs

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    Left-stuffer-1397C-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TALED_Right-stuffer-1397N-UGI

    Plasmid

    #187173

    Purpose

    original vector to make all constructs

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

    Insert

    Right-stuffer-1397N-UGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pATD

    Plasmid

    #141012

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    HSV-TK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUAS-FRT-GFP-FRT-uM-dCBEevoCDA1

    Plasmid

    #205152

    Purpose

    Inducible expression of dCBEevoCDA1 under control of Gal4/UAS and FLP/FRT

    Depositor

    Michael Boutros

    Article

    Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

    Insert

    FRT-GFP-FRT-uORF-dCBEevoCDA1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAST

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAV-U6+27-Tornado-biSiRA

    Plasmid

    #196345

    Purpose

    Overexpression of circular biSiRA

    Depositor

    Murat Sunbul

    Article

    Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

    Insert

    Tornado-biSiRA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6+27

    Available since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. P442

    Plasmid

    #139728

    Purpose

    Module plasmid, individual component of Fungal Bioluminescent Pathway, Luz, promoter is ABA responsive

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pMOD_D_pAtRAB18:NnLuz:tAtuOcs
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtRAB18

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pENTR-THT III

    Plasmid

    #55791

    Purpose

    GATEWAY cloning compatible conditional shRNA expression vector; TetO flanked H1 RNA gene promoter; Gentamycin resistance

    Depositor

    Stephan Geley

    Article

    Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

    Type

    Empty backbone

    Use

    RNAi; Gateway

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    modified H1 RNA gene

    Available since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET20b-GpNLuc-6xHis

    Plasmid

    #135932

    Purpose

    Expresses the GpNLuc LumiFluor in bacterial cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJin-264

    Plasmid

    #125717

    Purpose

    ABA-inducible split T7 gRNA for GFP vector using RNAPN d5-19

    Depositor

    Bryan Dickinson

    Article

    Pu et al ACS Chem Biol. 2018 Feb 16;13(2):431-437

    Insert

    PT7-gRNA GFP expression, PYL-linker-T7 RNAPC, T7 RNAPN (d5-19)-linker-ABI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

    Plasmid

    #101107

    Purpose

    Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2

    Depositor

    Tudor Fulga

    Article

    Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

    Insert

    BDKRB2-dCas9(N)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. CaYin1

    Plasmid

    #33067

    Depositor

    Robert Campbell

    Article

    Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

    Insert

    CaYin1
    

    Use

    Tags

    6 His tag

    Expression

    Bacterial

    Mutation

    Promoter

    ARA promoter

    Available since

    Jan. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCP5

    Plasmid

    #35397

    Depositor

    Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Type

    Empty backbone

    Use

    Reporter

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GDP promoter

    Available since

    Aug. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pFUS_A30A

    Plasmid

    #31029

    Depositor

    Adam Bogdanove, Daniel Voytas

    Article

    Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

    Insert

    LacZ + BsaI restriction sites
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. P223

    Plasmid

    #139720

    Purpose

    Module plasmid, individual component of Fungal Bioluminescent Pathway, CPH

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pMOD_A'_pLHB1B1:NnCPH:tSlH4
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtLHB1B1

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pFA6a-TRP1-PGAL1-HBH

    Plasmid

    #26886

    Depositor

    Peter Kaiser

    Article

    Tagwerker et al Yeast. 2006 Jun . 23(8):623-32.

    Insert

    HBH tag N-terminal
    

    Use

    Pcr tagging yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p663-UBC-miniTurbo-V5-DSTYK _IDG-K

    Plasmid

    #158182

    Purpose

    Gateway destination clone of DSTYK (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-DSTYK (DSTYK Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge