We narrowed to 9,389 results for: Coli

1. pSB3K3-CT81

   Plasmid

   #165404

   Purpose

   Bacteria gene circuit on medium copy backbone.

   Depositor

   Xiaojun Tian

   Article

   Zhang et al Nat Commun. 2021 Feb 8;12(1):853. doi: 1

   Insert

   CT81 gene circuit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSB3K3-CT66

   Plasmid

   #165405

   Purpose

   Bacteria gene circuit on medium copy backbone.

   Depositor

   Xiaojun Tian

   Article

   Zhang et al Nat Commun. 2021 Feb 8;12(1):853. doi: 1

   Insert

   CT66 gene circuit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBEL2080

   Plasmid

   #155160

   Purpose

   Expresses Strep-MlaF_L266A)-(6xHis-TEV-MlaB)

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   MlaF-(6xHis-TEV-MlaB)
   

   Use

   Tags

   Expression

   Mutation

   MlaF_L266A

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBEL2081

   Plasmid

   #155161

   Purpose

   Expresses (Strep-MlaF_Y261A_L265A_L266A)-(6xHis-TEV-MlaB)

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   MlaF-(6xHis-TEV-MlaB)
   

   Use

   Tags

   Expression

   Mutation

   MlaF(Y261A_L265A_L266A)

   Promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBEL1305

   Plasmid

   #155143

   Purpose

   Expresses MlaFB, His tag on MlaF N-term

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   (6xHis-TEV-MlaF)-MlaB
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBEL1306

   Plasmid

   #155144

   Purpose

   Expresses MlaFB, His tag on MlaF C-term

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   (MlaF-6xHis)-MlaB
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBEL1307

   Plasmid

   #155145

   Purpose

   Expresses MlaFB, His tag on MlaB N-term

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   MlaF-(6xHis-TEV-MlaB)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBEL1308

   Plasmid

   #155146

   Purpose

   Expresses MlaFB, His tag on MlaB C-term

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   MlaF-(MlaB-6xHis)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   T7 promoter

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBEL1833

   Plasmid

   #155149

   Purpose

   Expresses MlaF(1-250)-GCN4

   Depositor

   Damian Ekiert, Gira Bhabha

   Article

   Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

   Insert

   mlaF(1-250)-GCN4_Dimerization_Domain
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   araBAD

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBEL1957

    Plasmid

    #155151

    Purpose

    Expresses Strep-MlaF-(6xHis-TEV-MlaB)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    MlaF-(6xHis-TEV-MlaB)
    

    Use

    Tags

    Expression

    Mutation

    added Streptavidin tag on MlaF N-terminus

    Promoter

    T7 promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBEL2074

    Plasmid

    #155155

    Purpose

    Expresses Strep-MlaF-(6xHis-TEV-MlaB_T52E)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    MlaF-(6xHis-TEV-MlaB)
    

    Use

    Tags

    Expression

    Mutation

    MlaB_T52E

    Promoter

    T7 promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEL2076

    Plasmid

    #155156

    Purpose

    Expresses (Strep-MlaF_K47A)-(6xHis-TEV-MlaB)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    MlaF-(6xHis-TEV-MlaB)
    

    Use

    Tags

    Expression

    Mutation

    MlaF_K47A

    Promoter

    T7 promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBEL2077

    Plasmid

    #155157

    Purpose

    Expresses (Strep-MlaF_E170Q)-(6xHis-TEV-MlaB)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    MlaF-(6xHis-TEV-MlaB)
    

    Use

    Tags

    Expression

    Mutation

    MlaF_E170Q

    Promoter

    T7 promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBEL2078

    Plasmid

    #155158

    Purpose

    Expresses (Strep-MlaF_Y261A)-(6xHis-TEV-MlaB)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    MlaF-(6xHis-TEV-MlaB)
    

    Use

    Tags

    Expression

    Mutation

    MlaF_Y261A

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBEL2079

    Plasmid

    #155159

    Purpose

    Expresses (Strep-MlaF_L265A)-(6xHis-TEV-MlaB)

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

    Insert

    MlaF-(6xHis-TEV-MlaB)
    

    Use

    Tags

    Expression

    Mutation

    MlaF_L265A

    Promoter

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBEL2042

    Plasmid

    #139927

    Purpose

    Complementation plasmid for LetAB with 4 ring (rings 1347) LetB mutant

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetAB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetA-LetB (1-159; 280-515; 747-877)

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBEL2045

    Plasmid

    #139924

    Purpose

    Expression plasmid for 4 ring (rings 1347) LetB (without TM region) mutant

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB (43-159; 280-515; 747-877)

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBEL2002

    Plasmid

    #139887

    Purpose

    Expression plasmid for LetAB with 4 ring (rings 1567) LetB (without TM region) mutant

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; delta161-514)-6xHis

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBEL2001

    Plasmid

    #139886

    Purpose

    Expression plasmid for LetAB with 3 ring (rings 167) LetB (without TM region) mutant

    Depositor

    Damian Ekiert, Gira Bhabha

    Article

    Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

    Insert

    LetB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    LetB(43-877; delta161-634)-6xHis

    Promoter

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET29b-clbI S178A

    Plasmid

    #114159

    Purpose

    Expresses ClbI S178A

    Depositor

    Emily Balskus

    Article

    Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

    Insert

    clbI
    

    Use

    Tags

    6xHis and 6xHis-Thrombin site

    Expression

    Bacterial

    Mutation

    Mutated S178 to Ala

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTH861-gst-optec P210T

    Plasmid

    #89595

    Purpose

    Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed proline 210 to threonine

    Promoter

    T7

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTH846-gst-dissc

    Plasmid

    #96907

    Purpose

    Plasmid for yeast expression of GST encoded by non-preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    yeast codon dis-optimised glutathione-S transferase
    

    Use

    Tags

    8xhistidine

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTH860-gst-optec A180Q

    Plasmid

    #89594

    Purpose

    Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed alanine 180 to glutamine

    Promoter

    T7

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTH858-gst-optec V156E

    Plasmid

    #89592

    Purpose

    Plasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase V156E mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed valine 156 to glutamic acid

    Promoter

    T7

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTH859-gst-optec Y172M

    Plasmid

    #89593

    Purpose

    Plasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codons

    Depositor

    Tobias von der Haar

    Article

    Jossé et al bioRxiv 200006

    Insert

    glutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
    

    Use

    Tags

    8xhistidine

    Expression

    Bacterial

    Mutation

    changed tyrosine 172 to methionine

    Promoter

    T7

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKSJ375

    Plasmid

    #103064

    Purpose

    Expresses ImmE1, immunity to colicin E1

    Depositor

    Karen Jakes, Alan Finkelstein

    Article

    Jakes et al J Bacteriol. 2016 Dec 13;199(1). pii: e0

    Insert

    colicin E1 immunity protein (pColE1_26, imm E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    E1 immunity protein promoter from ColE1

    Available since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Ppj219(JBEI-13475)

    Plasmid

    #89785

    Purpose

    for expression of N-StrepII-3607

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    3607
    

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Ppj184 (JBEI-13431)

    Plasmid

    #89784

    Purpose

    for expression of N-His-3607

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    3607
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Ppj187 (JBEI-13437)

    Plasmid

    #89783

    Purpose

    for expression of C-His-3607

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Insert

    3607
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPJ169(JPUB_006769)

    Plasmid

    #83835

    Purpose

    Carries NEW operon 5 final

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Inserts

    Kuste3340
    Kuste3350
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    op5_3350_for and op5_BsaI_3340_for

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPJ170(JPUB_006771)

    Plasmid

    #83833

    Purpose

    Carries NEW operon 6 final

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Inserts

    Kuste3346
    Kuste3348
    Kuste3349
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    op6_3348_for, op6_3349_for, and op6_BsmBI_3346_fo…

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPJ168(JPUB_006767)

    Plasmid

    #83832

    Purpose

    Carries NEW operon 4 final

    Depositor

    Harry Beller

    Article

    Javidpour et al PLoS One. 2016 Mar 14;11(3):e0151087. do

    Inserts

    Kuste2802
    Kuste2805
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    op4_2805_for and op4_BsaI_2802_for

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBP-BBa_B0012

    Plasmid

    #72997

    Purpose

    BBa_B0012 iGEM terminator (5' TCGA/ 3' TGTT fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Bba_B0012 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBP-BBa_B0015

    Plasmid

    #72998

    Purpose

    BBa_B0015 iGEM terminator (5' TCGA/ 3' TGTT fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    BBa_B0015 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTU2_2b

    Plasmid

    #73011

    Purpose

    Secondary module (BpiI) site in pTU2b backbone

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Secondary module + Golden Gate pTU2b BsmBI destination site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBP-ORF-eCFP

    Plasmid

    #72961

    Purpose

    Level 0 Entry vector" ORF - eCFP"

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Enhanced cyan fluorescent protein (eCFP)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pBP-ORF-mCherry

    Plasmid

    #72962

    Purpose

    Level 0 Entry vector" ORF - mCherry "

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    mCherry fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pBP-SJM902

    Plasmid

    #72967

    Purpose

    Level 0 - SJM902 promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    SJM902 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

39. pBP-SJM903

    Plasmid

    #72968

    Purpose

    Level 0 - SJM903 promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    SJM903 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

40. pBP-SJM905

    Plasmid

    #72969

    Purpose

    Level 0 - SJM905 promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    SJM905 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only