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pGEX Csk-SH2

Plasmid

#46420

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Csk (CSK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 74-177

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-C1-FKBP-CYB5tail

Plasmid

#202686

Purpose

rapamycin-induced recruitment of proteins to ER

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

EGFP:FKBP:[GGSA]4GG:CYB5A(100-134) (CYB5A Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_mU6-sgASCL1_hU6-sgRNA_hUbC-PuroR-P2A-GFP

Plasmid

#162337

Purpose

Lentiviral expression of sgASCL1 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6; mU6

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas6

Plasmid

#126485

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas6

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX SHP-1(C)-SH2

Plasmid

#46494

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-1(C) (PTPN6 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 105-217

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX CrkL-SH2

Plasmid

#46419

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Crkl (CRKL Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 9-108

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas8b2

Plasmid

#126482

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas8b2

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas7

Plasmid

#126483

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas7

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-LmoCas5

Plasmid

#126484

Purpose

Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

LmoCas5

Use

CRISPR

Tags

Flag, NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX Crk-SH2

Plasmid

#46418

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Crk (CRK Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-124

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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CMV:: HA-hM4Dnrxn

Plasmid

#52525

Purpose

Expresses N-terminal HA tagged hM4Dnrxn

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

HA-hM4Dnrxn (CHRM4 Synthetic, Human)

Tags

HA

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX p85a(C)-SH2

Plasmid

#46463

Depositor

Leszek Kotula, Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85a(C) (PIK3R1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 612-724

Promoter

Tac

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX p85a(N)-SH2

Plasmid

#46464

Depositor

Leszek Kotula, Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85a(N) (PIK3R1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 321-434

Promoter

Tac

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SHP-1(N)-SH2

Plasmid

#46495

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-1(N) (PTPN6 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-109

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Fes-SH2

Plasmid

#46426

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Fes (FES Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 396-497

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmNanoLuc

Plasmid

#182969

Purpose

Stably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmNanoLuc

Expression

Mammalian

Mutation

N/A

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221 NLRP1 A66V

Plasmid

#166817

Purpose

Gateway pDONR vector encoding NLRP1 with the patient mutation A66V (Zhong et al., 2016) (no stop codon)

Depositor

Daniel Bachovchin

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

NLRP1 (NLRP1 Human)

Use

Gateway shuttling vector

Mutation

A66V

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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FL MALT1 Catalytic Mutant in pET29b-Strep

Plasmid

#48969

Purpose

Expresses Full length catalytic mutant MALT1 in e.coli

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 isoform A (MALT1 Human)

Tags

His and Strep

Expression

Bacterial

Mutation

Catalytic Mutant C464A

Promoter

T7

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
TRE-UBC-Crry-T2A-GFP

Plasmid

#205452

Purpose

lentiviral plasmid for expression of mouse Crry

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

Crry (Cr1l Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX Zap70(C)-SH2

Plasmid

#46533

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Zap70(C) (ZAP70 Human)

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 154-267

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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