We narrowed to 3,098 results for: actin

1. pQCXIH BRGI-Flag

   Plasmid

   #19148

   Depositor

   Joan Massague

   Article

   Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

   Insert

   SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pAG668 lifeAct-pFAST

   Plasmid

   #172866

   Purpose

   Expresses LifeAct-pFAST in mammalian cells (actin labeling)

   Depositor

   Arnaud Gautier

   Article

   Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

   Insert

   LifeAct-pFAST
   

   Use

   Tags

   Myc tag (C terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBa-eGFP-flag-BicD2 594-FKBP

   Plasmid

   #63569

   Purpose

   Bicaudal D protein aa1-594 (excluding start Met) tagged with N-terminal eGFP and flag and a C-terminal FKBP

   Depositor

   Marvin Bentley, Gary Banker

   Article

   Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

   Insert

   Bicaudal D protein (Bicd2 Mouse)
   

   Use

   Tags

   FKBP, eGFP, and flag

   Expression

   Mammalian

   Mutation

   no start methionine, aa1-594

   Promoter

   chicken Beta Actin

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENN.AAV.CB7.CI.ACY1-flag.WPRE.rBG

   Plasmid

   #132686

   Purpose

   AAV plasmid encoding mouse ACY1 with C-terminal flag tag

   Depositor

   Jonathan Long

   Article

   Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

   Insert

   Acylase I (Acy1, Acy1 Mouse)
   

   Use

   AAV

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Chicken b-actin

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Cas12f-GE ver4.0

   Plasmid

   #176543

   Purpose

   Expresses Cas12f-GE in mammalian cells

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

   Inserts

   Cas12f-GE ver4.0
   Cas12f-GE ver4.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and chicken β-actin

   Available since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHLmMBP-2

   Plasmid

   #72344

   Purpose

   Mammalian expression of secreted N-terminally 6His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

   Depositor

   Luca Jovine

   Article

   Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

   Type

   Empty backbone

   Use

   Tags

   6His-tag, HA-tag, Strep-tag II, and mMBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer + chicken beta-actin promoter

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Cas12f-GE ver3.0

   Plasmid

   #176508

   Purpose

   Expresses Cas12f-GE in mammalian cells

   Depositor

   Yong-Sam Kim

   Article

   Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

   Inserts

   Cas12f-GE ver3.0
   Cas12f-GE ver3.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and chicken β-actin

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCAGGS-SMS1-TEV-Twin-Strep

   Plasmid

   #202528

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell

   Depositor

   Chiaki Murakami

   Article

   Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

   Insert

   SGMS1 (SGMS1 Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted stop codon (TAA)

   Promoter

   CAG and chicken β-actin promoter

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. qTAG-N-Puro-mScarlet-ACTB

   Plasmid

   #207754

   Purpose

   Donor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-mScarlet Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. qTAG-N-Blast-mScarlet-ACTB

    Plasmid

    #207753

    Purpose

    Donor template for Blast-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    ACTB Homology Arms flanking a Blast-mScarlet Cassette (ACTB Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. mIFP12-sEspin-C-18

    Plasmid

    #56262

    Purpose

    Localization: Actin Bundling, Excitation: 683, Emission: 704

    Depositor

    Michael Davidson

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    sEspin
    

    Use

    Tags

    mIFP12

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pGBT-R16

    Plasmid

    #31827

    Depositor

    Stephen Ekker

    Article

    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

    Insert

    mini Tol2 ITR
    

    Use

    Cre/Lox; Gene trap

    Tags

    mRFP

    Expression

    Mutation

    mRFP lacks start codon

    Promoter

    beta Actin

    Available since

    Sept. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pS23.U6<ActB.mus_C-term-KI_gRNA

    Plasmid

    #207408

    Purpose

    gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

    Depositor

    Alexandros Poulopoulos

    Article

    Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

    Insert

    ActB
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pExpreS2-1-CR-PA-10H-SIII

    Plasmid

    #175447

    Purpose

    HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    BiP Secretion Peptide and Thrombin-Protein A-10Hi…

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCIG-FGFRdn

    Plasmid

    #80431

    Purpose

    Expression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryos

    Depositor

    Aixa Morales

    Article

    Martinez-Morales et al J Cell Biol. 2011 Aug 8;194(3):489-503.

    Insert

    Fibroblast growth factor receptor 1 (FGFR1 Chicken)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains aa 1–425

    Promoter

    beta-actin promoter

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

16. ArpC3-FLAG_pLib

    Plasmid

    #173680

    Purpose

    ArpC3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC3 (ARPC3 Human)
    

    Use

    Tags

    TEV cleavage site and FLAG tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ArpC1_pLib

    Plasmid

    #173678

    Purpose

    ArpC1 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    ArpC1 (ARPC1B Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMADM-alpha

    Plasmid

    #36890

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tdTomato-3Myc
    beta-globin intron
    Neo
    ATG (T)
    GFP
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, deleted…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 1183_pAAV-U6-Ex14-gRNAd-CB-EmGFP

    Plasmid

    #89061

    Purpose

    AAV-gRNA targeting the murine Ldlr gene

    Depositor

    William Lagor

    Article

    Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

    Insert

    EmGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin with partial CMV enhancer

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LK4-Ets2

    Plasmid

    #44606

    Depositor

    Robert Oshima

    Article

    Galang et al J Biol Chem. 1996 Apr 5;271(14):7992-8.

    Insert

    Ets2 (Ets2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human Beta-Actin

    Available since

    May 13, 2013

    Availability

    Academic Institutions and Nonprofits only