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pEnt L1L3 EF1a-tTA-2

Plasmid

#26803

Depositor

Edward Hsiao

Publication

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Elongation Factor 1 alpha promoter and tTA transgene

Use

Gateway Cloning

Expression

Mammalian

Available since

Feb. 23, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Flag-HA-USP3

Plasmid

#22582

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP3 (USP3 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pStart-K

Plasmid

#20346

Depositor

Mario Capecchi

Publication

Wu et al Nat Protoc. 2008 . 3(6):1056-76.

Type

Empty backbone

Use

Gateway Cloning and Mouse Targeting

Available since

March 11, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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AAVS1 pegRNA1 with trimmed attP

Plasmid

#222346

Purpose

pegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installation

Depositor

David Liu

Publication

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AAVS1 attP-installation pegRNA1

Use

CRISPR

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pRDA_550

Plasmid

#203398

Purpose

All-in-one lentiviral expression of gRNA and EnAsCas12a

Depositor

John Doench

Publication

Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

Insert

Enhanced Acidaminococcus Cas12a

Use

CRISPR and Lentiviral

Promoter

EF1a

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-PE2max-BSD

Plasmid

#191102

Purpose

Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Publication

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

PEmax-P2A-BSD

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Changed R221K, N394K, H840A from SpCas9

Promoter

EF-1a

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Fos-CreERT2

Plasmid

#194643

Purpose

Expresses Cre-ERT2 under the control of c-Fos promoter

Depositor

Jun-Hyeong Cho

Publication

Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

Insert

Fos-CreERT2

Use

AAV

Expression

Mammalian

Promoter

c-Fos

Available since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLminP_Luc2P_RE1

Plasmid

#90335

Purpose

NFkB - p50/p65 pathway gene reporter

Depositor

Ramnik Xavier

Publication

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

NFKB

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR223 SARS-CoV-2 NSP12

Plasmid

#141265

Purpose

Gateway-compatible Entry vector, with insert of NSP12 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP12 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Gene ID 43740578)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti CMV TRE3G Neo GFP-Progerin

Plasmid

#118710

Purpose

Lentiviral TET-ON inducible GFP-Progerin

Depositor

Tom Misteli

Publication

Kubben et al Methods. 2015 Sep 1. pii: S1046-2023(15)

Insert

GFP-Progerin (LMNA Human)

Use

Lentiviral; Tet-on inducible

Tags

GFP

Expression

Mammalian

Promoter

CMV TRE3G (TET-ON)

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLenti-ABERA-P2A-Puro

Plasmid

#112675

Purpose

Lentivirus for constitutive expression of ABE7.10 RA (codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

ABERA

Use

Lentiviral

Tags

FLAG

Mutation

D10A

Promoter

EF1s

Available since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pMJ826

Plasmid

#39316

Depositor

Jennifer Doudna

Publication

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Histidine 840 to Alanine (H840A)

Promoter

T7

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pLenti-PGK-NRAS(G12V)

Plasmid

#35632

Depositor

Daniel Haber

Publication

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

N-Ras(G12V) (NRAS Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pCFJ352 - MCS(ttTi4348, I)

Plasmid

#34868

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi4348 targeting

Use

Worm targeting

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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Flag-HA-OTUD1

Plasmid

#22553

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

OTUD1 (OTUD1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti X2 Puro DEST (w16-1)

Plasmid

#17296

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Puro

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning, Lentiviral, and RNAi

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEX-CMV-SP-YFP-STM2(15-746)

Plasmid

#18862

Depositor

Tobias Meyer

Publication

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Stromal interaction molecule 2 (STIM2 Human)

Tags

YFP

Expression

Mammalian

Mutation

YFP inserted between signal peptide(SP) of STIM1 …

Available since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pAF0033 Ef1a-Pa01-2a-EGFP

Plasmid

#193460

Purpose

Expression of Pa01 recombinase from Ef1a promoter

Depositor

Patrick Hsu

Publication

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01

Expression

Mammalian

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTwist-SARS-CoV-2 Δ18 B.1.1.529

Plasmid

#179907

Purpose

Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Publication

Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014

Insert

SARS-CoV-2 B.1.1.529 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

A67V; Δ69-70; T95I; G142D; Δ143-145; N211I; Δ212;…

Promoter

CAG

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX

Plasmid

#167582

Purpose

Express the ATP sensor GRAB_ATP1.0 in mammalian cells

Depositor

Yulong Li

Publication

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Expression

Mammalian

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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