We narrowed to 62,913 results for: cloning

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Ezh2[FL]-dCas9

Plasmid

#100086

Purpose

Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Publication

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

14056 (Ezh2 Mouse)

Use

CRISPR

Tags

3XFLag-NLS-Ezh2[FL]-dCas9-NLS

Expression

Mammalian

Promoter

CMV

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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plenti-CAG -IRES-GFP

Plasmid

#69047

Purpose

Lentiviral backbone for GFP coexpression

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral

Tags

IRES-GFP

Expression

Mammalian

Available since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMJ179

Plasmid

#85996

Purpose

One-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2

Depositor

Jonathan Weissman

Publication

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

EGFP-NT2_cr2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified mouse U6

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pMJ114

Plasmid

#85995

Purpose

One-guide Perturb-seq vector backbone; modified bovine U6 promoter; original constant region

Depositor

Jonathan Weissman

Publication

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

modified bovine U6-2

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pLentiPGK Puro DEST PKAKTRClover

Plasmid

#59153

Purpose

Lentiviral vector to express PKA KTR mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Publication

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

PKA (PRKACA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pFRT-TODestFLAGHAhFXR1

Plasmid

#48694

Purpose

Expresses hFXR1

Depositor

Thomas Tuschl

Publication

Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

Insert

FlagHA FXR1 (isoform b) (FXR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available since

June 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-PGK-NRAS(G12V)

Plasmid

#35632

Depositor

Daniel Haber

Publication

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

N-Ras(G12V) (NRAS Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
pCFJ352 - MCS(ttTi4348, I)

Plasmid

#34868

Depositor

Erik Jorgensen

Publication

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi4348 targeting

Use

Worm targeting

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pMXs-OLIG2

Plasmid

#32933

Depositor

Kevin Eggan

Publication

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

OLIG2 (OLIG2 Human)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Flag-HA-USP49

Plasmid

#22586

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP49 (USP49 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Flag-HA-OTUD1

Plasmid

#22553

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

OTUD1 (OTUD1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCG150

Plasmid

#17247

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

unc-119 rescue fragment in pDESTR4R3

Use

Gateway Cloning

Available since

March 28, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDest-544

Plasmid

#11519

Depositor

Dominic Esposito

Type

Empty backbone

Use

Gateway Cloning

Tags

His6-NusA

Expression

Bacterial

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pET His6 High pI protein 69 TEV LIC cloning vector (2-HpI-69)

Plasmid

#55212

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, High pI protein 69, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pET FLAG TEV cloning vector with BioBrick polycistronic restriction sites (9L)

Plasmid

#48288

Depositor

Scott Gradia

Type

Empty backbone

Tags

FLAG

Expression

Bacterial

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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AAVS1 pegRNA1 with trimmed attP

Plasmid

#222346

Purpose

pegRNA 1 plasmid used for PASSIGE-mediated Bxb1 attP installation

Depositor

David Liu

Publication

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Insert

AAVS1 attP-installation pegRNA1

Use

CRISPR

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-PE2max-BSD

Plasmid

#191102

Purpose

Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Publication

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

PEmax-P2A-BSD

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Changed R221K, N394K, H840A from SpCas9

Promoter

EF-1a

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVX-Puro-hGSDME

Plasmid

#154876

Purpose

Expresses hGSDME in mammalian cells

Depositor

Judy Lieberman

Publication

Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

Insert

GSDME (GSDME Human)

Use

Lentiviral

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

Plasmid

#138152

Purpose

Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

Depositor

Neville Sanjana

Publication

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

eGFP-MCS

Use

Lentiviral

Tags

2PEST AND NLS

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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YE1-BE4max

Plasmid

#138155

Purpose

C-to-T base editor

Depositor

David Liu

Publication

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

Promoter

CMV

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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