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Plasmid#191397PurposesgRNA targeting alpha-satellites on human chromosome 9DepositorInsertsgRNA targeting alpha satellites on human chromosome 9
UseLentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-eGFP-SynaptoZip
Plasmid#122525PurposeAAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoterDepositorInserteGFP-SynaptoZip (Vamp2 Rat)
UseAAVTagsMyc and eGFPExpressionMammalianMutationPromoterhSynAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNATM3.1/V5-His TOPO-PKCi-K274W
Plasmid#55690Purposeexpresses dominant negative human PKCi tagged with V5DepositorInsertHomo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
UseTagsV5/HisExpressionMammalianMutationA120E and K274WPromoterCMVAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-RaichuEV-Rac1(T17N)
Plasmid#164902PurposeExpresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domainDepositorInsertRaichuEV-Rac1 (RAC1 Human)
UseTagsExpressionMammalianMutationT17N mutation in the Rac1 domain which make the s…PromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMyc4ElbLuc.
Plasmid#53246Purposereporter plasmid containing 4 myc cDNA binding sitesDepositorInsertpMyc4ElbLuc (MYC Human)
UseTagsExpressionMutationTo prepare plasmids with one, two, three, and fou…PromoterAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-4CL5-AtSCT (JBEI-11557)
Plasmid#87937PurposeCo-expression in S.cerevisiae of 4CL5 and AtSCTDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNATM3.1/V5-His TOPO-PKCi-A120E
Plasmid#55687Purposeexpresses constitutively active human PKCi tagged with V5DepositorInsertHomo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
UseTagsV5/HisExpressionMammalianMutationA120EPromoterCMVAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK5 HA-GST-Presc-mAkt1 (wt)
Plasmid#48805Purposeexpresses wt Akt1DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHA-GST-PrescExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGZ173 (pFA6a-SL-MNase-kanMX6)
Plasmid#70234PurposeChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitopeDepositorInsertShort linker and aa 83-231 of Mnase
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMyc3ElbLuc
Plasmid#53245Purposereporter plasmid containing 3 myc cDNA binding sitesDepositorInsertpMyc3ElbLuc (MYC Human)
UseTagsExpressionMutationTo prepare plasmids with one, two, three, and fou…PromoterAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-TDAG51
Plasmid#32699DepositorInserthuman tdag51 (PHLDA1 Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3HA-ErbB4CTF
Plasmid#17803DepositorInsertErbB4 (ERBB4 Human)
UseTagsHAExpressionMammalianMutationCarboxy-terminal fragment, amino acids 676-1292PromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGZ110 (pFA6a-3FLAG-MNase-TRP1)
Plasmid#70233PurposeChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope tagDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMyc1ElbLuc,
Plasmid#53243Purposereporter plasmid containing 1 myc cDNA binding sitesDepositorInsertpMyc1ElbLuc (MYC Human)
UseTagsExpressionMutationTo prepare plasmids with one, two, three, and fou…PromoterAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pKM60
Plasmid#154159PurposeFirefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.DepositorInsertFirefly luciferase
UseLuciferaseTagsExpressionMutation4 wt NF-κB sites were replaced with Rel1A-specifi…PromoterAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pM-ErbB4CTF
Plasmid#17797DepositorInsertErbB4 (ERBB4 Human)
UseTagsGAL4-BDExpressionMammalianMutationCarboxy-terminal fragment only, amino acids 676-1…PromoterAvailable sinceMay 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
XW17
Plasmid#65835PurposePunc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)DepositorInsertOR74A quinic acid utilization activator (qa-1F)
UseTagsmCherryExpressionWormMutationThe endogenous SphI, KpnI and SacI sites in QF ar…PromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcFLAG-CSK-R107E
Plasmid#74503PurposeOverexpression of CSK R107E mutant in mammalian cellsDepositorInsertCSK (CSK Human)
UseTagsFLAGExpressionMammalianMutationR107E, K222RPromoterCMVAvailable sinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-S6K1(T389S) barcoded
Plasmid#48800Purposeexpresses mutant S6K1DepositorInsertS6K1 T389S (Rps6kb1 Rat)
UseLentiviralTagsFLAG and barcode: GGTACCExpressionMammalianMutationT389SPromoterCMVAvailable sinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfCsf1a
Plasmid#168103PurposeExpress soluble His-tagged zebrafish Csf1a in insect cellsDepositorInsertCsf1a (csf1a Zebrafish)
UseTags6x HisExpressionInsectMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only