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Showing: 241 - 270 of 17327 results

  4. pAAV-hSyn-GRAB_OTmut

    Plasmid
    #185388
    Purpose
    Expresses the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons
    Depositor
    Yulong Li
    Article
    Qian et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Has Service
    AAV1 and AAV9
    Insert
    GPCR activation based oxytocin control sensor GRAB_OTmut
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Sept. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185388-plasmid-map-sequence-id-372581

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  7. pGL3 basic Mut E2

    Plasmid
    #48748
    Purpose
    Expression of luciferase driven by mPer2 promoter fragment containing mutated E-box2 (bases -112 to +98 with respect to transcription start site at +1)
    Depositor
    Joseph Takahashi
    Article
    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10
    Insert
    mPer2 promoter/enhancer region containing mutated E-box2 (-112 to +98)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Mutated E-box2 sequence from CACGTT to GCTAGT
    Promoter
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    48748-plasmid-map-sequence-id-448240

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  9. pAAV-hSyn-GRAB_NEmut

    Plasmid
    #123310
    Purpose
    Expresses the genetically-encoded fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons
    Depositor
    Yulong Li
    Article
    Feng et al Neuron. 2019 May 22;102(4):745-761.e8. d
    Has Service
    AAV Retrograde and AAV9
    Insert
    GPCR activation based NE control sensor GRAB_NEmut
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    123310-plasmid-map-sequence-id-240667

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  10. pmIL-6 mut AP-1+C/EBP

    Plasmid
    #61292
    Purpose
    drives luciferase from mouse IL-6 promoter with mutations in both AP-1 & C/EBP sites
    Depositor
    Gail Bishop
    Article
    Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.
    Insert
    IL-6 promoter (Il6 Mouse)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Mutated both AP-1 binding site from TGAGTCT to TG…
    Promoter
    IL-6 promoter with mutant AP-1 and C/EBP binding …
    Available since
    May 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pINDUCER dCas9-TET1CDMut

    Plasmid
    #101920
    Purpose
    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.
    Depositor
    Danwei Huangfu
    Article
    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10
    Insert
    dCas9-TET1CD Mut
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Mutated TET1 catalytic domain
    Promoter
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    101920-plasmid-map-sequence-id-194554

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  19. pcDNA3-CBX1mut-PA-mCherry

    Plasmid
    #138251
    Purpose
    Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.
    Depositor
    Jin Zhang
    Article
    Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:
    Insert
    CBX1mut-PAmCherry
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    138251-plasmid-map-sequence-id-307435

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  23. pET-APOBEC1-YTHmut

    Plasmid
    #200159
    Purpose
    For bacterial expression of the APOBEC1-YTHmut fusion protein for protein purification
    Depositor
    Kate Meyer
    Article
    Zhu et al Front Cell Dev Biol. 2022 Apr 27;10:8882
    Insert
    APOBEC1-YTHmut
    Use
    Tags
    6xHis, HA, and MBP
    Expression
    Bacterial
    Mutation
    Codon optimized for E coli expression
    Promoter
    LacZ
    Available since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    200159-plasmid-map-sequence-id-396325

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  28. pET-Sfp R4-4 mutant

    Plasmid
    #173491
    Purpose
    Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.
    Depositor
    Jun Yin
    Article
    Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d
    Insert
    Sfp-R44
    Use
    Tags
    His tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    173491-plasmid-map-sequence-id-349047

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  29. pAAV-hSyn-GRAB_CRFmut

    Plasmid
    #208659
    Purpose
    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in neurons
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    hSyn
    Available since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    208659-plasmid-map-sequence-id-410350

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  30. DBDmut-CA-RIT-NFAT1

    Plasmid
    #63669
    Purpose
    Constitutively active NFAT1 and also unable to interact with AP-1 with four point mutations in the DNA binding loop that abolish DNA binding
    Depositor
    Anjana Rao
    Article
    Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15
    Insert
    NFAT1 (Nfatc2 Mouse)
    Use
    Retroviral
    Tags
    3x HA
    Expression
    Mammalian
    Mutation
    CA: PASSGSSASF mutated to PAAAGAAAAF, SPRTSPIMSP…
    Promoter
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 241 - 270 of 17327 results