We narrowed to 2,492 results for: ERK

1. EKARdual

   Plasmid

   #99870

   Purpose

   Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

   Depositor

   Marc Tramier

   Article

   Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

   Insert

   EKAR
   

   Use

   Tags

   ShadowG and mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-EKAR4

   Plasmid

   #174437

   Purpose

   Improved cyan/yellow FRET-based ERK kinase activity reporter.

   Depositor

   Jin Zhang

   Article

   Keyes et al eLife. 2020 May 26;9. pii: 57410. doi: 1

   Insert

   EKAR4
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS-4511NES

   Plasmid

   #138375

   Purpose

   Booster-ERK, a Red-Shifted Genetically Encoded FRET Biosensor for ERK activity.

   Depositor

   Michiyuki Matsuda

   Article

   Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

   Insert

   Booster-ERK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-endo-EKAR4

   Plasmid

   #205513

   Purpose

   Genetically encoded FRET-based biosensor for monitoring ERK kinase activity. Targeted to endosomes.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-EKAR4
   

   Use

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and 6xHI…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MTK3_030

   Plasmid

   #123744

   Purpose

   Encodes ERK SPARK, a ERK activity reporter, as a Type 3 part to be used in the MTK system

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   ERK SPARK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. R777-E125 Hs.MAPK1

   Plasmid

   #70409

   Purpose

   Gateway ORF clone of human MAPK1 [NM_002745.4] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. R777-E126 Hs.MAPK1-nostop

   Plasmid

   #70410

   Purpose

   Gateway ORF clone of human MAPK1 [NM_002745.4] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pToneDest

   Plasmid

   #67691

   Purpose

   attR4-attR3 Destination cassette in Tol1 backbone

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pToneTP

   Plasmid

   #67692

   Purpose

   For in vitro transcription of mRNA encoding Tol1 Transposase

   Depositor

   Nathan Lawson

   Article

   Shin et al Development. 2016 Oct 15;143(20):3785-37

   Insert

   Tol1 transposase
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AKARet-PSD

    Plasmid

    #114732

    Purpose

    PSD-targeted AKARet

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    PSD-targeted AKARet
    

    Use

    Tags

    EGFP and N-terminus of PSD-95

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. negEKARet-cyto

    Plasmid

    #114734

    Purpose

    Negative control of EKARet-cyto

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    Negative Control of EKARet-cyto
    

    Use

    Tags

    EGFP and sREAChet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EKARet-PSD

    Plasmid

    #114723

    Purpose

    PSD-targeted EKARet

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    PSD-targeted EKARet
    

    Use

    Tags

    EGFP and N-terminus of PSD-95

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. negAKARet-cyto

    Plasmid

    #114733

    Purpose

    Negative control of AKARet-cyto

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    Negative Control of AKARet-cyto
    

    Use

    Tags

    EGFP and sREAChet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pME-6MTnlstdTomato2A

    Plasmid

    #67685

    Purpose

    Middle entry nuclear localized tdTomato reporter with 6xMT and 2A

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Insert

    tdTomato
    

    Use

    Tags

    2A and 6xMyc-NLS

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmTol-ins-UAS-R1R3-CC

    Plasmid

    #67745

    Purpose

    miniTol Destination vector with MAR-flanked UAS

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3796-38

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MAPK1

    Plasmid

    #73248

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MAPK1 (MAPK1 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 3xERRE-luciferase

    Plasmid

    #37851

    Depositor

    Rebecca Riggins

    Article

    Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

    Insert

    3 copies of ERRE upstream of luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AKARet-cyto

    Plasmid

    #96897

    Purpose

    FLIM sensor for PKA activation in cytosol

    Depositor

    Ryohei Yasuda

    Article

    Tang et al Neuron. 2017 Mar 22;93(6):1315-1324.e3.

    Insert

    FLIM Sensor for PKA Activity in Cytosol
    

    Use

    Tags

    EGFP and sREAChet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p3EHA

    Plasmid

    #67708

    Purpose

    3' entry HA tag

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    HA tag
    

    Use

    Tags

    HA

    Expression

    Mutation

    Promoter

    None

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS-Fv2Eflt4-2a-tdTomato

    Plasmid

    #67697

    Purpose

    Expression construct

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    Fv2E-flt4-2A-tdTomato
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only