We narrowed to 22,295 results for: ESC;

1. His6-TEV-mCerulean3-pBluescript

   Plasmid

   #166757

   Purpose

   For purification of mCerulean3 fluorophore

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   mCerulean3
   

   Tags

   6x Histidine tag, and TEV protease site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. His6-TEV-Venus-pBluescript

   Plasmid

   #166756

   Purpose

   For purification of Venus fluorophore

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   Venus
   

   Tags

   6x Histidine tag, and TEV protease site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEF-pIgH-ESCargo(FTV)

   Plasmid

   #140160

   Purpose

   Expresses a regulatable secretory cargo for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
   

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pESC-URA-GFP-Ubc9ts

   Plasmid

   #20369

   Depositor

   Judith Frydman

   Article

   Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

   Insert

   SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
   

   Use

   Yeast/bacteria shuttle

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Y68L This mutation renders Ubc9 temperature-sens…

   Available Since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pESC-LEU-GFP-VHL

   Plasmid

   #21053

   Depositor

   Judith Frydman

   Article

   Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

   Insert

   von Hippel-Lindau tumor suppressor (VHL Human)
   

   Use

   Yeast/bacteria shuttle

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pBluescript-IC3-FLAG-Tev-ZZ

   Plasmid

   #170311

   Purpose

   Plasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.

   Depositor

   Andrew Carter

   Article

   Mali et al Science. 2021 Feb 26;371(6532):910-916.

   Insert

   Outer Dynein Arm Intermediate Chain 3
   

   Tags

   FLAG, ZZ

   Available Since

   July 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pESC-URA::CYP71AV1/CPR

   Plasmid

   #20118

   Depositor

   Jay Keasling

   Article

   Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

   Insert

   amorphadiene oxidase and p450 reductase
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Available Since

   March 2, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pBluescript II KS(+) ACMV DNA-A 1.4mer

   Plasmid

   #159134

   Purpose

   Infectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-A

   Depositor

   Vincent Fondong

   Article

   Hoyer et al Microbiol Resour Announc. 2020 Nov 12;9(

   Insert

   ACMV DNA-A 1.4mer
   

   Use

   Infectious clone for bombarding plants

   Available Since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pESC-URA-GFP-Ubc9wt

   Plasmid

   #20368

   Depositor

   Judith Frydman

   Article

   Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

   Insert

   SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
   

   Use

   Yeast/bacteria shuttle

   Tags

   GFP

   Expression

   Yeast

   Available Since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

10. plko-shmfn2-mfn2-Rescue

    Plasmid

    #160655

    Purpose

    "Rescue mfn2 expression by adding the guide-resistant-mutant mfn2 sequence"

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    mfn2 (MFN2 Human)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Pet15b-Endosomal Escape Control

    Plasmid

    #186552

    Purpose

    Negative Control confirming the need for a CPP peptide in mediating internalization

    Depositor

    Jean Gariepy

    Article

    Mohammed et al J Control Release. 2012 Nov 28;164(1):58

    Insert

    6H-ETA-GFP
    

    Tags

    6xHis and EGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Pet15b-Internalization and Endosomal Escape

    Plasmid

    #186551

    Purpose

    Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

    Depositor

    Jean Gariepy

    Article

    Mohammed et al J Control Release. 2012 Nov 28;164(1):58

    Insert

    10R-8His-ETA-EGFP
    

    Tags

    8xHis and EGFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTAJAK-71 (pESC-NatMXsyn-USER)

    Plasmid

    #78232

    Purpose

    Empty vector, for the insertion of gRNA expression cassettes into the vector.

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pESC-URA-Rnq1-GFP

    Plasmid

    #21051

    Depositor

    Judith Frydman

    Article

    Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

    Insert

    Rnq1 (RNQ1 Budding Yeast)
    

    Use

    Yeast/bacteria shuttle

    Tags

    GFP

    Expression

    Yeast

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pRK5 HA-GST-Presc-mAkt1 (wt)

    Plasmid

    #48805

    Purpose

    expresses wt Akt1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    Akt1 (Akt1 Mouse)
    

    Tags

    HA-GST-Presc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pRK5-myc-PICK1-rescue

    Plasmid

    #72573

    Purpose

    Expresses myc-PICK1 shRNA resistant cDNA

    Depositor

    Victor Anggono, Richard Huganir

    Article

    Anggono et al J Neurosci. 2011 Feb 9;31(6):2188-96. do

    Insert

    PICK1 (Pick1 Rat)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    5 silent mutations around shRNA recognition seque…

    Promoter

    CMV

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pESC-Leu-SopB(C460S)

    Plasmid

    #183671

    Purpose

    Inducible Salmonella Typhimurium SopB (catalytically inactive) for expression in yeast

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    sopB (sopB Salmonella enterica serovar Typhimurium)
    

    Expression

    Yeast

    Mutation

    C460S

    Promoter

    GAL1

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pESC-NAT-Ago-Dcr

    Plasmid

    #160381

    Purpose

    Overexpression of Ago-Dcr fusion protein in yeast

    Depositor

    John McCusker

    Insert

    Ago- Dcr
    

    Expression

    Yeast

    Promoter

    GAL

    Available Since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBlueScript II SK-SNAP donor for H3f3b

    Plasmid

    #186933

    Purpose

    Genomic targeting of SNAP tag at H3f3b C-terminal

    Depositor

    Zhiguo Zhang

    Article

    Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

    Insert

    SNAP donor for H3f3b (H3f3b Mouse)
    

    Tags

    SNAP tag

    Expression

    Mammalian

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag-TRIM24-C840W shRescue

    Plasmid

    #28137

    Depositor

    Michelle Barton

    Article

    Tsai et al Nature. 2010 Dec 16. 468(7326):927-32.

    Insert

    TRIM24 (TRIM24 Human)
    

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    C840Wmutated at aa 308 and 309 for shRNA rescue; …

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only