We narrowed to 1,049 results for: IM

1. HP138-neo

   Plasmid

   #134247

   Purpose

   Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selection

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

   Insert

   Contains Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-GFP1-10-HygR

   Plasmid

   #223675

   Purpose

   To check the split GFP signals in mitochondria (1)

   Depositor

   Juan Melero-Martin

   Article

   Lin et al Nature. 2024 May;629(8012):660-668. doi:

   Insert

   GFP1-10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PB-GFP11x7-HygR

   Plasmid

   #223676

   Purpose

   To check the split GFP signals in mitochondria (2)

   Depositor

   Juan Melero-Martin

   Article

   Lin et al Nature. 2024 May;629(8012):660-668. doi:

   Insert

   GFP11x7
   

   Use

   Tags

   GFP11

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCit-4aa-SNAP25B

   Plasmid

   #53235

   Purpose

   Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   SNAP-25B (Snap25 Rat)
   

   Use

   Tags

   4aa-linker and mCitrine

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTK23

   Plasmid

   #37405

   Depositor

   Iain Cheeseman

   Article

   Kiyomitsu et al Nat Cell Biol. 2012 Feb 12;14(3):311-7.

   Insert

   Neuromodulin (GAP43 Human)
   

   Use

   Tags

   S-tag, TEV, and mCherry

   Expression

   Mammalian

   Mutation

   Contains the first 20aa of GAP43 (neuromodulin)

   Promoter

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLNHA-C1-HseIF4A3_I

   Plasmid

   #146578

   Purpose

   Mammalian Expression of HseIF4A3

   Depositor

   Elisa Izaurralde

   Article

   Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

   Insert

   HseIF4A3 (EIF4A3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJS183

   Plasmid

   #134142

   Purpose

   Bacterial expression of GFP-Ska1 and Ska2

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   His-GFP-human Ska1; human Ska2
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mCer-4aa-VAMP2

   Plasmid

   #53233

   Purpose

   Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Use

   Tags

   4aa-linker and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pCB004

   Plasmid

   #134147

   Purpose

   Bacterial expression of human Ska3

   Depositor

   Iain Cheeseman

   Article

   Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

   Insert

   human Ska3 (SKA3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pT7-EGFP-C1-HseIF4A3_I

    Plasmid

    #146581

    Purpose

    Mammalian Expression of HseIF4A3

    Depositor

    Elisa Izaurralde

    Article

    Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

    Insert

    HseIF4A3 (EIF4A3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Stx1a-12aa-mCer

    Plasmid

    #53236

    Purpose

    Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    Syntaxin 1A (Stx1a Rat)
    

    Use

    Tags

    12aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pDEST17-ScChd1(118-1274)

    Plasmid

    #221207

    Purpose

    ScChd1-aa118-1274 chromatin remodeler

    Depositor

    Gregory Bowman

    Article

    Nodelman et al Nat Struct Mol Biol. 2022 Feb;29(2):121-

    Insert

    ScChd1 (aa118-1274)
    

    Use

    Tags

    6xHis-tag (Precission protease cleavable)

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-117 and 1275-1468

    Promoter

    Available since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC008 - pBR322 with tetR-inducible RFP

    Plasmid

    #79157

    Purpose

    inducible RFP expression vector

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    TetR + RFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTomo-AurA

    Plasmid

    #26293

    Depositor

    Inder Verma

    Article

    Marumoto et al Nat Med. 2009 Jan . 15(1):110-6.

    Insert

    aurora kinase A (AURKA Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pT7-EGFP-C1-HsMago_I

    Plasmid

    #146582

    Purpose

    Mammalian Expression of HsMago

    Depositor

    Elisa Izaurralde

    Article

    Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

    Insert

    HsMago (MAGOH Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    one non silent mutation Y123C compared to the seq…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. H2B-mScarlet- hRec8(297-506)-mNeonGreen

    Plasmid

    #174717

    Purpose

    Stable expression of fluorescent Separase cleavage sensor with human Rec8

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Rec8 (REC8 Human)
    

    Use

    Retroviral

    Tags

    H2B-mScarlet and mNeonGreen

    Expression

    Mammalian

    Mutation

    297-506

    Promoter

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. NavB4-GFP/pEGFP-N1

    Plasmid

    #197310

    Purpose

    Plasmid expressing an antigen targeting the Nav beta4 sodium channel subunit

    Depositor

    Lori Isom

    Article

    Aman et al J Neurosci. 2009 Feb 18;29(7):2027-42. d

    Insert

    NavB4-GFP (Scn4b Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKG209

    Plasmid

    #45109

    Depositor

    Iain Cheeseman

    Article

    Gascoigne et al Cell. 2011 Apr 29;145(3):410-22. doi: 10

    Insert

    CENP-T (aa1-374) Laci-NLS (CENPT Human)
    

    Use

    Tags

    GFP, Laci-NLS, s peptide, and tev

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CENP-B mCherry PP1 gamma

    Plasmid

    #45221

    Depositor

    Iain Cheeseman, Michael Lampson

    Article

    Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

    Insert

    CENP-B mCherry PP1 gamma
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. C-term Hec1-targeted Aurora B FRET sensor

    Plasmid

    #45222

    Depositor

    Michael Lampson

    Article

    Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

    Insert

    C-term Hec1-targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge