We narrowed to 24,396 results for: Spr

1. pCRISPR

   Plasmid

   #186276

   Purpose

   Plasmid containing shortened version of upstream Thiomicrospira CRISPR array for integration assays

   Depositor

   Jennifer Doudna

   Article

   Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

   Insert

   Thiomicrospira shortened CRISPR array
   

   Expression

   Bacterial

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETM33_SPSB1_SPRY

   Plasmid

   #178487

   Purpose

   Bacterial expression of human domain with His-tag and GST tag

   Depositor

   Ylva Ivarsson

   Article

   Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

   Insert

   SPSB1_SPRY (SPSB1 Synthetic, Human)
   

   Tags

   His-GST

   Expression

   Bacterial

   Promoter

   T7/LacO

   Available Since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLentiCRISPRv1_COX4

   Plasmid

   #177983

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting COX4

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting COX4
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti_CRISPRi_sgAR10

   Plasmid

   #167003

   Purpose

   Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   AR Promoter sgRNA 10 (AR Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHH0103_SPRED3_WH1

   Plasmid

   #109899

   Purpose

   Protein expression and purification of SPRED3_WH1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   SPRED3_WH1 (SPRED3 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHH0103_SPRED2_WH1

   Plasmid

   #109825

   Purpose

   Protein expression and purification of SPRED2_WH1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   SPRED2_WH1 (SPRED2 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHH0103_SPRED1_WH1

   Plasmid

   #109816

   Purpose

   Protein expression and purification of SPRED1_WH1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

   Insert

   SPRED1_WH1 (SPRED1 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lentiCRISPRv2_PROX1_1+4

   Plasmid

   #121177

   Purpose

   Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

   Depositor

   Timo Otonkoski

   Insert

   Cas9-P2A-puro and U6 promoters driven expression of gRNAs
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A-puro

   Expression

   Mammalian

   Available Since

   March 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LentiCRISPR_Hs_MBD3_ex2

   Plasmid

   #62623

   Purpose

   Encodes gRNA which targets human MBD3

   Depositor

   Paul Wade

   Insert

   MBD3 gRNA (MBD3 Human)
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS

   Available Since

   Nov. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

10. LentiCRISPR_Hs_MBD3_ex3

    Plasmid

    #62624

    Purpose

    Encodes gRNA which targets human MBD3

    Depositor

    Paul Wade

    Insert

    MBD3 gRNA (MBD3 Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    EFS

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

11. LentiCRISPR v2 mBAX

    Plasmid

    #129577

    Purpose

    CRISPR deletion of murine BAX

    Depositor

    Stephen Tait

    Article

    Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

    Insert

    gRNA vs murine BAX (Bax Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LentiCRISPR v2 mBAK

    Plasmid

    #129578

    Purpose

    CRISPR deletion of murine BAK

    Depositor

    Stephen Tait

    Article

    Lopez et al Nat Commun. 2016 Feb 2;7:10538. doi: 10.

    Insert

    gRNA vs murine BAK (Bak1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYLCRISPR/Cas9Pubi-H

    Plasmid

    #66187

    Purpose

    expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    maize ubiquitin promoter (Pubi)

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CRISPRoff-v2.1

    Plasmid

    #167981

    Purpose

    Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

    Depositor

    Luke Gilbert

    Article

    Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

    Insert

    CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
    

    Tags

    2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lentiCRISPR v2-Blast

    Plasmid

    #83480

    Purpose

    Mammalian expression of Cas9 and sgRNA scaffold

    Depositor

    Mohan Babu

    Insert

    Blasticidin S deaminase
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    EF-1α

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. LentiCRISPRv2-mCherry

    Plasmid

    #99154

    Purpose

    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.

    Depositor

    Agata Smogorzewska

    Inserts

    Cas9
    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPR-Cas3

    Plasmid

    #209427

    Purpose

    Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

    Depositor

    Tilmann Weber

    Article

    Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

    Insert

    codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    PtipA; crRNA under control of PermE*

    Available Since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. ABE8.20-SpRY

    Plasmid

    #226859

    Purpose

    Expresses ABE8.20-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8.20-SpRY
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lentiCRISPRv2 neo

    Plasmid

    #98292

    Purpose

    Variant of lentiCRISPRv2 that confers G418 resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF-1a

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiCRISPRv2-Opti

    Plasmid

    #163126

    Purpose

    LentiCRISPRv2 variant with modified sgRNA scaffold from Chen et al. 2013 Cell.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge