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pTRIP-CMV-mtagBFP2-2A-FLAG-cGAS E225A/D227A

Plasmid

#102604

Purpose

Lentivector to express Flag-tagged and catalytically-inactive cGAS and mTagBFP

Depositor

Nicolas Manel

Article

Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E225A-D227A

Promoter

CMV

Available since

Jan. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Q498R (CGA)

Plasmid

#212475

Purpose

Encodes SARS-CoV-2 spike Q498R for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Q498R (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Q498R

Promoter

CAG

Available since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 P681R (CGA)

Plasmid

#212569

Purpose

Encodes SARS-CoV-2 spike P681R for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike P681R (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

P681R

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Q493R (CGA)

Plasmid

#212473

Purpose

Encodes SARS-CoV-2 spike Q493R for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Q493R (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Q493R

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Q52R (CGA)

Plasmid

#212394

Purpose

Encodes SARS-CoV-2 spike Q52R for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Q52R (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Q52R

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Q239R (CGA)

Plasmid

#212375

Purpose

Encodes SARS-CoV-2 spike Q239R for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Q239R (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Q239R

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pHW564 (Pmyo-2::cGAL-N(C1A)::let-858 3'UTR)

Plasmid

#107739

Purpose

mutated cGAL-N (C1A) driver under the control of C. elegans myo-2 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein(C1A)

Use

Tags

Expression

Worm

Mutation

change the first cysteine of gp41-1 N-intein to a…

Promoter

C. elegans myo-2 promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS-FLAG- cGAS E225A/D227A

Plasmid

#127660

Purpose

Over-express catalytically inactive EGFP-NLS-FLAG-cGAS E225A/D227A

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

Expression

Mutation

E225A/D227A

Promoter

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-synYFP[TTG/AGA]-4xArg[CGA]-nLuc

Plasmid

#199759

Purpose

Elongation reporter construct to quantify elongation duration of 4_CGA stalling

Depositor

Brian Zid

Article

Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

Insert

synYFP[TTG/AGA]-4xArg[CGA]-nLuc

Use

Tags

Expression

Yeast

Mutation

Promoter

TetO7

Available since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc

Plasmid

#199760

Purpose

Elongation reporter construct to quantify elongation duration of 6_CGA stalling

Depositor

Brian Zid

Article

Hou et al bioRxiv. 2023 Mar 20:2023.03.19.533377.

Insert

synYFP[TTG/AGA]-6xArg[CGA]-nLuc

Use

Tags

Expression

Yeast

Mutation

Promoter

TetO7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 Q239R (CGA) D614G (GGT)

Plasmid

#212378

Purpose

Encodes SARS-CoV-2 spike Q239R, D614G for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike Q239R, D614G (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

Q239R, D614G

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)

Plasmid

#174516

Purpose

Recoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Inserts

PylS_1R26 - CBZK
Af_TyrRS - p-I-Phe
Ma tRNA pylT(8) with CGA anticodon
A.fulgidus tRNA with CUA anticodon

Use

Tags

Expression

Bacterial

Mutation

CUA anticodon, M.alvus Pyl tRNA with CGA anticodo…

Promoter

GlnS and lpp

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIB-H2B-mCherry

Plasmid

#164244

Purpose

retroviral plasmid for H2B-mCherry

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

H2B

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIZ-turq-3xFLAG-TREX1

Plasmid

#164232

Purpose

retroviral plasmid for turq-3xFLAG-TREX1

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Retroviral

Tags

FLAG and mturquoise

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIP GFP-TREX1

Plasmid

#164245

Purpose

retroviral plasmid for GFP-TREX1

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIP-GFP-RPA70

Plasmid

#164231

Purpose

retroviral plasmid for GFP-RPA70

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

RPA70 (RPA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-p53 gRNA-Cas9-T2A-mCherry

Plasmid

#164256

Purpose

Transcription of p53 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sg p53

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-TREX1 g2 Cas9-T2A-mCherry

Plasmid

#164251

Purpose

Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgTREX1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-TREX1 g1 Cas9-T2A-mCherry

Plasmid

#164250

Purpose

Transcription of TREX1 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgTREX1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pQCXIZ 3xFLAG-TREX1

Plasmid

#164243

Purpose

retroviral plasmid for 3xFLAG-TREX1

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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