Addgene

We narrowed to 141,980 results for: ins

Showing: 161 - 180 of 141980 results

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pHAGE-EGFR-A763_Y764insFQEA

Plasmid

#116233

Purpose

Lentiviral expression of EGFR A763_Y764insFQEA

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Expression

Mutation

A763_Y764insFQEA

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-Y764_V765insHH

Plasmid

#116319

Purpose

Lentiviral expression of EGFR Y764_V765insHH

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Expression

Mutation

Y764_V765insHH

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHCKan-INSyibp-Nef

Plasmid

#134593

Purpose

Low Phosphate inducible Nef

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

HIV Nef protein

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

yibD

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRosa26-4xInsulator (JP6)

Plasmid

#99627

Purpose

IfgMosaic acceptor vector for Classical or Cas9 Gene targeting of the Rosa26 locus

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Gt(Rosa)26Sor (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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TwinStrep-SUMO-Pdp1

Plasmid

#198320

Purpose

For affinity purification of Pdp1 payload

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

TwinStrep-SUMO-Pdp1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pInsert-PhoCl-RER

Plasmid

#219676

Purpose

Caging at cytosolic C-ter of POI: mScarletCD4-PhoCl-RER in pTREtight2

Depositor

Helge Ewers

Article

Kashyap et al Nat Methods. 2024 Mar 8. doi: 10.1038/s4

Insert

CD4mScarlet-PhoCl-RER

Use

Tags

mScarlet and PhoCl

Expression

Mammalian

Mutation

Promoter

TRE

Available since

June 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEx-insertion-tet

Plasmid

#172211

Purpose

For genetic insertion in tetracyclin-sensitive Prevotella copri strains

Depositor

Till Strowig

Article

Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

Type

Empty backbone

Use

Genetic insertion in prevotella copri

Tags

Expression

Mutation

Promoter

Available since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBPK1520-HEK3-CTTins-ng

Plasmid

#180019

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of HEK3 CTT insertion in PE3 approach

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing ngRNA for HEK3-CTTins (EPHA8 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human U7

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBluescriptII[attB/Ins1]

Plasmid

#74100

Purpose

"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)

Depositor

Ben Szaro

Article

Wang et al Dev Biol. 2015 Feb 1;398(1):11-23. doi:

Inserts

Phi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR ASVinsertion

Plasmid

#32066

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Insert duplicate A767-V769

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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TwinStrep-SUMO-Pnf

Plasmid

#198321

Purpose

For affinity purification of Pnf payload

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

TwinStrep-SUMO-Pnf

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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4xHRE_minSV40-sfGFP-CMV_dsRed

Plasmid

#117400

Purpose

sfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoter

Depositor

Yvonne Chen

Article

Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

Insert

SuperFolder GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-E746_T751delinsA

Plasmid

#116246

Purpose

Lentiviral expression of EGFR E746_T751delinsA

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Expression

Mutation

E746_T751delinsA

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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ins-cre_cryaa-Venus

Plasmid

#24333

Depositor

Didier Stainier

Article

Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

Insert

Cre (cre )

Use

Cre/Lox

Tags

Venus

Expression

Worm

Mutation

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-E709_T710delinsD

Plasmid

#116239

Purpose

Lentiviral expression of EGFR E709_T710delinsD

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Expression

Mutation

E709_T710delinsD

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR insertionH

Plasmid

#32067

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Insert H at H773-V774

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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Endophilin SH3 domains

Plasmid

#47401

Depositor

Peter McPherson

Article

Micheva et al J Biol Chem. 1997 Oct 24;272(43):27239-4

Insert

Endophilin

Use

Tags

GST

Expression

Bacterial

Mutation

SH3 domain (aa 292-352)

Promoter

tac

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
R777-E107 Hs.INSRR

Plasmid

#70391

Purpose

Gateway ORF clone of human INSRR [NM_014215.2] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

INSRR (INSRR Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-L747_A750delinsP

Plasmid

#116267

Purpose

Lentiviral expression of EGFR L747_A750delinsP

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Expression

Mutation

L747_A750delinsP

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Sumo3 PolQM1 deltains1

Plasmid

#78461

Purpose

Inducible expression in E. coli

Depositor

Sylvie Doublie, Susan Wallace

Article

Hogg et al J Mol Biol. 2011 Jan 21;405(3):642-52. d

Insert

Polymerase theta (POLQ Human)

Use

Tags

His and SUMO3

Expression

Bacterial

Mutation

delta 1-1791; delta 2149-2170; Q2513R (please see…

Promoter

T7

Available since

July 28, 2016

Availability

Academic Institutions and Nonprofits only