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Showing: 161 - 180 of 446 results

  1. pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197064
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197064-plasmid-map-sequence-id-402605

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  2. pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156434
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    All zinc fingers mutated
    Promoter
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156434-plasmid-map-sequence-id-310270

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  3. pAAVS1_TREtight-GEM4-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197062
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM4-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197062-plasmid-map-sequence-id-402606

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  4. pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197063
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197063-plasmid-map-sequence-id-408409

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  9. pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-EFS-rtTA-P2A-mGL-WPRE

    Plasmid
    #235304
    Purpose
    Lentiviral expression of ComMAND open-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)
    Depositor
    Kate Galloway
    Article
    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1
    Insert
    FXN-P2A-mRuby2 (FXN Human)
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G (3rd-generation Tet system)
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235304-plasmid-map-sequence-id-472752

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  12. pLentiX1-[TRE3G-FXN-P2A-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-EFS-rtTA-P2A-mGL-WPRE

    Plasmid
    #235305
    Purpose
    Lentiviral expression of ComMAND closed-loop circuit regulating FXN-P2A-mRuby2 (therapeutically relevant gene)
    Depositor
    Kate Galloway
    Article
    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1
    Insert
    FXN-P2A-mRuby2 (FXN Human)
    Use
    Lentiviral and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G (3rd-generation Tet system)
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235305-plasmid-map-sequence-id-472751

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  13. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156429
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    DELTA1-265
    Promoter
    Available since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156429-plasmid-map-sequence-id-310209

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  14. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #156432
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156432-plasmid-map-sequence-id-310156

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  15. KL094 - pTRE3G-Flag-Halo-CTCF_WT-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #232930
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF WT, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection.
    Depositor
    Danny Reinberg
    Article
    Lucero et al bioRxiv, March 19, 2025
    Insert
    Flag-Halo-CTCF WT
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232930-plasmid-map-sequence-id-466502

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  16. KL095 - pTRE3G-Flag-Halo-CTCF_ZF1_mutant-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #232931
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF ZF1 mutant, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection.
    Depositor
    Danny Reinberg
    Article
    Lucero et al bioRxiv, March 19, 2025
    Insert
    Flag-Halo-CTCF ZF1 deletion mutant
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Deleted amino acids 264-277
    Promoter
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232931-plasmid-map-sequence-id-466490

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Showing: 161 - 180 of 446 results