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p15A_J23108_SpydCas9_CmR

Plasmid

#119163

Purpose

Expresses S. pyogenes dCas9

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

dCas9 from S. pyogenes

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER ERbeta shRNA

Plasmid

#35561

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2009 Feb;23(2):146-56. E

Insert

ERbeta

Use

Retroviral

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only
pAT773

Plasmid

#68941

Purpose

NanoLuc expressed constiutively from PAM1 and GH022 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-OCT4

Plasmid

#35391

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE-Oct4

Use

Lentiviral

Expression

Mammalian

Available Since

May 1, 2012

Availability

Academic Institutions and Nonprofits only
LV UAS-DsRed-Express2-PGK-Puro

Plasmid

#190801

Purpose

LV construct encoding UAS-DsRed-Express2 reporter gene.

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

UAS-T2A-DsRed-Express2

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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GB4356

Plasmid

#187813

Purpose

Module for dCasEV2.1 activated expression of Atr delta11, HarFAR and SpATF1-2.

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Moth Pheromone pathway

Expression

Plant

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pAT796

Plasmid

#68929

Purpose

NanoLuc expressed constiutively from PAM4 and rpiL* RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAT695

Plasmid

#68910

Purpose

NanoLuc expressed constiutively from PBT1311 and RC500 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAT587

Plasmid

#68906

Purpose

NanoLuc expressed constiutively from PBT1311 and GH022 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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Cas9m3

Plasmid

#47315

Purpose

Cas9 D10A+D839A+H840A

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m3

Use

CRISPR

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
NTERY06

Plasmid

#184124

Purpose

Nanopore-addressable protein reporter for expression in bacterial cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

NTERY06

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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mNTERY00

Plasmid

#184110

Purpose

Nanopore-addressable protein reporter for expression in mammalian cells.

Depositor

Jeff Nivala

Article

Cardozo et al Nat Biotechnol. 2022 Jan;40(1):42-46. do

Insert

mNTERY00

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pDL14

Plasmid

#17837

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

gpdA promoter, lacZ, trpC

Use

Synthetic Biology; A. nidulans

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pCoofy59

Plasmid

#122011

Purpose

Baculovirus vector for parallel SLIC cloning containing N-terminal TrxA-His6 with PreScission Cleavage site

Depositor

Peggy Stolt-Bergner, Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

TrxA-His6

Expression

Insect

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2Ab

Plasmid

#183082

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Use

Synthetic Biology

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMM580

Plasmid

#68883

Purpose

NanoLuc expressed constiutively from PcepA, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAT779

Plasmid

#68916

Purpose

NanoLuc expressed constiutively from PAM2 and GH078 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pBbE7c-XAacxnT (JBEI-14118)

Plasmid

#100961

Purpose

XAacx operon in pBbE7c

Depositor

Jay Keasling

Article

Brown et al ACS Synth Biol. 2016 Dec 16;5(12):1485-1

Insert

XAacx operon

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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maturase-RT

Plasmid

#181805

Purpose

E.r. maturase RT

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2022 Apr 4. pii: 10.1038

Insert

maturase-RT

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAT782

Plasmid

#68919

Purpose

NanoLuc expressed constiutively from PAM2 and rpiL* RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pECT1

Plasmid

#75446

Purpose

Plasmid that serves as the PCR template to create genomic pdt1 insertions. Plasmid contains the R6K origin of replication so needs pir gene to replicate

Depositor

James Collins

Article

Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

Insert

pdt1

Expression

Bacterial

Promoter

none

Available Since

Oct. 5, 2016

Availability

Academic Institutions and Nonprofits only

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IgM (exon 1-3)

Plasmid

#11301

Depositor

Lisa Steiner

Article

Danilova et al Nat Immunol. 2005 Mar . 6(3):295-302.

Insert

IgM (ighm Zebrafish)

Expression

Bacterial

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pM1GG

Plasmid

#108200

Purpose

M. sedula PCNA1

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

M. sedula PCNA1

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAT795

Plasmid

#68928

Purpose

NanoLuc expressed constiutively from PAM4 and GH023 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pDL11

Plasmid

#17832

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

alcA promoter, lacZ, argB,

Use

Synthetic Biology; Aspergillus nidulans expression

Available Since

May 28, 2008

Availability

Academic Institutions and Nonprofits only
pMM596

Plasmid

#68885

Purpose

IPTG-inducible NanoLuc expression, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Inserts

NanoLuc
LacI

Expression

Bacterial

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pTLR-donor-tetO

Plasmid

#119000

Purpose

Repair template for the TLR reporter with 7 tet operator elements

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

mVenus

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDSAG

Plasmid

#62289

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, GFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pAT590

Plasmid

#68908

Purpose

NanoLuc expressed constiutively from PBT1311 and GH049 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAT780

Plasmid

#68917

Purpose

NanoLuc expressed constiutively from PAM2 and GH022 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAT793

Plasmid

#68926

Purpose

NanoLuc expressed constiutively from PAM4 and GH078 RBS, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pDSARN

Plasmid

#62295

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed-NLS marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pMK-S. tuberosum trnI/trnA

Plasmid

#123583

Purpose

MoChlo kit

Depositor

Scott Lenaghan, C. Neal Stewart

Article

Occhialini et al Plant Physiol. 2019 Jan 24. pii: pp.18.0

Insert

S. tuberosum trnI/trnA integration site

Use

Chloroplast transformation vector

Expression

Plant

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDSAT

Plasmid

#62290

Purpose

docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, mTurquoise CFP marker

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Type

Empty backbone

Expression

Insect

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWp7-3

Plasmid

#72735

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-[miR-132]

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-CMVp-GFP-[miR-132]

Use

Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pAWp7-7

Plasmid

#72739

Purpose

pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP

Depositor

Timothy Lu

Article

Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

Insert

UBCp-RFP-[miR-124 sensor]-CMVp-GFP

Use

Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL13

Plasmid

#17836

Depositor

Jay Keasling

Article

Lubertozzi et al Appl Microbiol Biotechnol. 2006 Oct . 72

Insert

alcA promoter, lacZ, trpC

Use

Synthetic Biology; A. nidulans

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
IgZ secreted

Plasmid

#11302

Depositor

Lisa Steiner

Article

Danilova et al Nat Immunol. 2005 Mar . 6(3):295-302.

Insert

IgZ (ighz Zebrafish)

Expression

Bacterial

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pAT752

Plasmid

#68903

Purpose

NanoLuc expressed constiutively from PAM2, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pAT754

Plasmid

#68905

Purpose

NanoLuc expressed constiutively from PAM4, pNBU2 backbone, AmpR

Depositor

Timothy Lu, Christopher Voigt

Article

Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

Insert

NanoLuc

Expression

Bacterial

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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