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pX330A_FokI-1x3
Plasmid#63603PurposeExpresses FokI-dCas9 and gRNADepositorInsertFokI-dCas9
UseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMM750
Plasmid#68899PurposeIPTG-inducible CRISPRi vector targeting PcfiA (PR2), constiutive NanoLuc, pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
NanoLuc
UseTagsExpressionMutationPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
YSBE 731 : pHYG_4xFLAG
Plasmid#92090PurposePlasmid for tagging proteins in Cryptococcus neoformans; 4xFLAG tag, HYG selectionDepositorInsert4xFlag
Usetagging proteins in cryptococcus neoformanTagsExpressionMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pX330A_dCas9-1x5
Plasmid#63599PurposeExpresses dCas9 and gRNADepositorInserthumanized S. pyogenes dCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
1xNLS-pMJ915v2-sfGFP
Plasmid#88919PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
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2xNLS-pMJ915v2-sfGFP
Plasmid#88920PurposeFor expression of modified SpyCas9 in e.coli.DepositorInsertsCas9
T7
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDL12c
Plasmid#17835DepositorInsertgpdA promoter, lacZ, argB_1626,
UseSynthetic Biology; Aspergillus nidulans expressionTagsExpressionMutationThe argB_1626 mutation consists of two sequential…PromoterAvailable sinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
p413GPD-hTPI Lys13Arg
Plasmid#50722Purposeexpression of the human TPI Lys13Arg allele in yeastDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTagsExpressionYeastMutationchanged Lysine 13 to ArgeninePromoterGPDAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
p413GPD-hTPI Ile170Thr
Plasmid#50721Purposeexpression of the human TPI Ile170Thr allele in yeastDepositorInsertTriosephosphate isomerase 1 (TPI1 Human)
UseTagsExpressionYeastMutationIsoleucine 170 to ThreoninePromoterGPDAvailable sinceFeb. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB4780
Plasmid#106143PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA2
Plasmid#64984PurposeExpresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag and S tagExpressionBacterialMutationdeleted amino acids 1-59, E105K, E108APromotertetracycline promoter/operatorAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330A_dCas9-1x3
Plasmid#63597PurposeExpresses dCas9 and gRNADepositorInserthumanized S. pyogenes dCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
TALEN-ID12-T
Plasmid#41479DepositorInsertTALEN-FokI nuclease backbone
UseTags3xFLAG and WT FokI nuclease domainExpressionMammalianMutationPromoterCMVAvailable sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
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TAL3112
Plasmid#41286DepositorInsertZebrafishCommunity-mcu-Left (zgc:153607 Zebrafish)
Uset7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
TAL3113
Plasmid#41287DepositorInsertZebrafishCommunity-mcu-Right (zgc:153607 Zebrafish)
Uset7Tags3X Flag and WT FOKIExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNH030
Plasmid#42149DepositorInsertmTFP1::linker::2xNLS
UseTagsExpressionWormMutationPromoterAvailable sinceFeb. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pASKt15C+SrtAwoH
Plasmid#65017PurposeExpresses the wild type of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsS tagExpressionBacterialMutationdeleted amino acids 1-59Promotertetracycline promoter/operatorAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
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