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pAAV-pEF1a-rtTAc

Plasmid

#172121

Purpose

Constitutive expression of InteinC-rtTAC

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

InteinC-rtTAc

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCytERM-EBFP2

Plasmid

#184939

Purpose

ER Visualization (OSER assay) Expresses EBFP2 in fusion with N-terminal part of p450

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

CytERM-EBFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pIhh-5A9-LUC

Plasmid

#46318

Depositor

Xiangli Yang

Article

Wang et al Development. 2009 Dec;136(24):4143-53. d

Insert

5 copies of A9 sequence from Ihh promoter

Use

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

mouse osteocalcin gene 2 promoter

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
PB-CAG-4300-L1-G1397N

Plasmid

#210396

Purpose

To correct m.A4300G mutation on mtDNA

Depositor

Bin Shen

Article

Chen et al Commun Biol. 2023 Nov 3;6(1):1116. doi:

Insert

4300-L1-G1397N

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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PB-CAG-4300-H2-G1397C

Plasmid

#210395

Purpose

To correct m.A4300G mutation on mtDNA

Depositor

Bin Shen

Article

Chen et al Commun Biol. 2023 Nov 3;6(1):1116. doi:

Insert

4300-H2-G1397C

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-LexA-CIB1-biLINuS

Plasmid

#192607

Purpose

Lentiviral vector for constitutive expression of LexA-CIB1-biLINuS

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

LexA-CIB1-biLINuS

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-PGK-eGFP-P2A-CRY2-VPR

Plasmid

#192610

Purpose

Lentiviral vector for constitutive expression of eGFP-P2A-CRY2-VPR.

Depositor

Yingxiao Wang

Article

Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

Insert

eGFP-P2A-CRY2PHR-VPR

Use

Lentiviral and Synthetic Biology

Tags

eGFP

Expression

Mutation

Promoter

PGK

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pElectra1-LaminA

Plasmid

#179482

Purpose

Nuclear envelope labeling with blue fluorescent protein Electra1

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1-LaminA

Use

Tags

Electra1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pRabies-EBFP-tTAn

Plasmid

#172120

Purpose

Expression of EBFP and tTA-InteinN in Rabies virus infected cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

EBFP, tTAn-InteinN

Use

Rabies virus

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRabies-EGFP-rtTAn

Plasmid

#172119

Purpose

Expression of EGFP and rtTA-InteinN in Rabies virus infected cells

Depositor

Chun Xu

Article

Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

Insert

EGFP, rtTAn-InteinN

Use

Rabies virus

Tags

Expression

Mutation

Promoter

CMV

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA2

Plasmid

#99735

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-racRNA-A

Plasmid

#200824

Purpose

AAV transfer plasmid encoding a circularized RNA barcode A under U6 promoter and a subcellular localization protein binder under hSyn promoter

Depositor

Xiao Wang

Article

Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

Inserts

U6-racRNA
V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far

Use

AAV

Tags

C-Far, Flag, and V5

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Icam 2 SaCas9 YAP sgRNA1

Plasmid

#99734

Purpose

Expresses SaCas9 and a gRNA targeting mouse YAP

Depositor

Yu Huang

Article

Wang et al Nature. 2016 Dec 7. doi: 10.1038/nature2

Insert

SaCas9 gRNA targeting Yap1 (Yap1 Mouse)

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-racRNA-B

Plasmid

#200825

Purpose

AAV transfer plasmid encoding a circularized RNA barcode B under U6 promoter and a subcellular localization protein binder under hSyn promoter

Depositor

Xiao Wang

Article

Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

Inserts

U6-racRNA
V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far

Use

AAV

Tags

C-Far, Flag, and V5

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-racRNA-C

Plasmid

#200826

Purpose

AAV transfer plasmid encoding a circularized RNA barcode C under U6 promoter and a subcellular localization protein binder under hSyn promoter

Depositor

Xiao Wang

Article

Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

Inserts

U6-racRNA
V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far

Use

AAV

Tags

C-Far, Flag, and V5

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-racRNA-D

Plasmid

#200827

Purpose

AAV transfer plasmid encoding a circularized RNA barcode D under U6 promoter and a subcellular localization protein binder under hSyn promoter

Depositor

Xiao Wang

Article

Shi et al Nature. 2023 Oct;622(7983):552-561. doi:

Inserts

U6-racRNA
V5-PP7cp-M9-NES-T2A-Flag-PP7cp-Far

Use

AAV

Tags

C-Far, Flag, and V5

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TMEM230-M4/X184PG-IRES(isoform 2)

Plasmid

#99568

Purpose

mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

M4/X184PG

Promoter

CMV

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-M3/Y92C-IRES(isoform 2)

Plasmid

#99567

Purpose

mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

M3/Y92C

Promoter

CMV

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-M2/X184W-IRES(isoform 2)

Plasmid

#99566

Purpose

mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

M2/X184W

Promoter

CMV

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM230-M1/R141L-IRES(isoform 2)

Plasmid

#99565

Purpose

mammalian expression of mutant TMEM230 (isoform 2) and ZsGreen

Depositor

Han-Xiang Deng

Article

Deng et al Nat Genet. 2016 Jul;48(7):733-9. doi: 10

Insert

TMEM230 (TMEM230 Human)

Use

Tags

Expression

Mammalian

Mutation

M1/R141L

Promoter

CMV

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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