Addgene

We narrowed to 28,366 results for: PLE

Showing: 1781 - 1800 of 28366 results

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plenti-px330-CRBN-T2-pGK-Pur

Plasmid

#107383

Purpose

Mammalian expression CRISPR/Cas9

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

sgRNA targeting CRBN (CRBN Human)

Use

Lentiviral; Crispr

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti MS2-p65-HSF1 sfGFP

Plasmid

#171051

Purpose

Expression of Vp64-dCas9 activation system

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

MS2-p65-HSF1_sfGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-RAP1A-E63-puro

Plasmid

#156167

Purpose

Lentiviral vector for expression of RAP1A-E63, with puromycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1A (RAP1A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

changed Glutamine 63 to Glutamic acid

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-eArch 3.0-EYFP

Plasmid

#35514

Purpose

Lentiviral expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eArch3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Enhanced trafficking signal and ER export signal …

Promoter

CaMKIIa

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Puro DEST JNKKTR EE Clover

Plasmid

#90239

Purpose

Lentiviral vector to express JNK KTR EE (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (EE mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

Plasmid

#71217

Purpose

dCas9 fused to human DNMT3B catalytic domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3B catalytic domain

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-PtenK13E/K289E-Puro

Plasmid

#135681

Purpose

Constitutive expression (cMV promoter) of K13E/K289E mutant Pten cDNA, Puromycin selection

Depositor

Florian Karreth

Insert

Pten (Pten Mouse)

Use

Lentiviral

Tags

Expression

Mutation

K13E/K289E

Promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-EGFP-ALDH1A3-Neo

Plasmid

#189744

Purpose

The construct was used to express GFP tagged human ALDH1A3 in mammalian cells for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A3 (ALDH1A3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGAS R71/75E-HA

Plasmid

#130919

Purpose

Expresses human cGAS-HA with R71/75E point mutation; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

human cGAS R71/75E

Use

Tags

HA

Expression

Mammalian

Mutation

aa 71 and 75 mutated to glutamate

Promoter

CMV

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE3G-BE3-PGK-Puro

Plasmid

#110845

Purpose

Lentiviral vector for dox-inducible expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Tags

Expression

Mutation

D10A

Promoter

TRE3G

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CaMKIIa-Mac 3.0-EYFP

Plasmid

#35515

Purpose

Lentiviral expression of CaMKIIa-driven Mac 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eMac 3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Trafficking signal, ER export

Promoter

CaMKIIa

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-lox-GFP shRNA p19-2

Plasmid

#14091

Depositor

Tyler Jacks

Article

Sage et al Nature. 2003 Jul 10. 424(6945):223-8.

Insert

p19 ARF shRNA -2 (Cdkn2a Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pLentiPGK Puro DEST JNKKTR AE Clover

Plasmid

#90240

Purpose

Lentiviral vector to express JNK KTR AE (mutant) mClover under PGK promoter (With Puromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

JNK Kinase Translocation Reporter (AE mutant)

Use

Lentiviral

Tags

mClover

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Neo/pTER shEGFP#2 (w22-2)

Plasmid

#17480

Purpose

3rd gen lentiviral eGFP shRNA #2 (H1/TO promoter), 3’LTR insertion, Neo

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #2

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLEX-FLAG-NRAS-IRES-Puro

Plasmid

#120574

Purpose

Expresses Flag-N-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pCRII-Topo Plexin D1 in situ probe

Plasmid

#45621

Depositor

Jeffrey Macklis

Article

Molyneaux et al Neuron. 2005 Sep 15;47(6):817-31.

Insert

Plexin D1 in situ probe (Plxnd1 Mouse)

Use

In situ

Tags

Expression

Mutation

fragment contains bp# 5751-6406 of NM_026376.3

Promoter

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
pLenti-PGK-RAP1A-E63-neo

Plasmid

#156174

Purpose

Lentiviral vector for expression of RAP1A-E63, with neomycin selection

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

RAP1A (RAP1A Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Mutation

changed Glutamine 63 to Glutamic acid

Promoter

human PGK

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-GFP-miRE_shRen-Blast

Plasmid

#135682

Purpose

Constitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection

Depositor

Florian Karreth

Insert

shRenilla

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)

Plasmid

#55199

Purpose

CFP reporter along with FF4 miRNA binding sites and 28 nt sequences

Depositor

Timothy Lu

Article

Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

Insert

ECFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-HA-YWAA-pGK-HYG

Plasmid

#107379

Purpose

Lentiviral expression of HA-YWAA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

cereblon (CRBN Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

CRBN-Y383A/W385A

Promoter

UbcP

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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