We narrowed to 17,743 results for: RET

1. Stx1a-12aa-mCer

   Plasmid

   #53236

   Purpose

   Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   Syntaxin 1A (Stx1a Rat)
   

   Tags

   12aa-linker and mCerulean

   Expression

   Mammalian

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pDJSVT86

   Plasmid

   #108965

   Purpose

   Express periplasmic, outer membrane, or secreted proteins in E. coli - OmpA signal and 6His tag

   Depositor

   Daniel Slade

   Article

   Casasanta et al J Biol Chem. 2017 Dec 8;292(49):20240-20

   Type

   Empty backbone

   Tags

   OmpA 1-27, 6His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3TERM_Nos-35S

   Plasmid

   #136116

   Purpose

   3TERM after CDS/ CDS12-CTAG

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   3TERM_Nos-35S (A. tumefaciens, CaMV)
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. Mito-Lapronic/pGW1

   Plasmid

   #140754

   Purpose

   FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

   Depositor

   Luis Felipe Barros

   Article

   Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

   Insert

   Mito-Lapronic
   

   Tags

   Mitochondrial Sequence Signal

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF-pIgH-ESCargo(FTV)

   Plasmid

   #140160

   Purpose

   Expresses a regulatable secretory cargo for mammalian cells

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
   

   Tags

   ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCk3_spacer

   Plasmid

   #136073

   Purpose

   Use when making even level constructs (L2,...) if pCk3 position (position 3) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Odd spacer
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. mKOkappa-2A-mTurquoise2

   Plasmid

   #98837

   Purpose

   Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   mKOkappa-2A-mTurquoise2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. PROM5_MpEF1a

   Plasmid

   #136113

   Purpose

   Strong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notches

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   PROM5_MpEF1a Mapoly0024s0116
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Available Since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. Dimer cleaved

   Plasmid

   #198199

   Purpose

   FRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavage

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   mVenus-T2A-mTurquoise2
   

   Expression

   Mammalian

   Mutation

   A206K both

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLS.491

    Plasmid

    #184956

    Purpose

    Test effect of a1/a2 length on editing rpoB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12
    

    Expression

    Bacterial

    Mutation

    rpoB donor T1534C

    Promoter

    T7/lac

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-EGFP-C1-HsAGO1_F

    Plasmid

    #146314

    Purpose

    Mammalian Expression of HsAGO1

    Depositor

    Elisa Izaurralde

    Article

    Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

    Insert

    HsAGO1 (AGO1 Human)
    

    Expression

    Mammalian

    Mutation

    N574S

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

    Plasmid

    #140168

    Purpose

    Expresses a regulatable secretory cargo for Tetrahymena thermophila

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)
    

    Use

    Other

    Promoter

    MTT1

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF-pIgH-ESCargo(FTV,Crimson)

    Plasmid

    #140162

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
    

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. mNeonGreen-IETD-NanoLuc

    Plasmid

    #98288

    Purpose

    caspase-8 BRET Sensor

    Depositor

    Maarten Merkx

    Article

    den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

    Insert

    mNeonGreen-LEHD-NanoLuc
    

    Tags

    His-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

    Plasmid

    #185678

    Purpose

    Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-HaloTag-KKMP
    

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH7m34GW-35S:SNACS S785A

    Plasmid

    #203577

    Purpose

    Plant SnRK2S30A kinase activity FRET sensor in plant expression vector

    Depositor

    Julian Schroeder

    Article

    Zhang et al eLife. 2020 May 28;9:e56351. doi: 10.755

    Insert

    SNACS S785A
    

    Tags

    StrepII

    Expression

    Plant

    Mutation

    S785A

    Available Since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCsC_spacer

    Plasmid

    #136077

    Purpose

    Use when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Even spacer
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pCsE_spacer

    Plasmid

    #136078

    Purpose

    Use when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Even spacer
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pCsB

    Plasmid

    #136068

    Purpose

    Acceptor plasmid for even level position 2, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pCsC

    Plasmid

    #136069

    Purpose

    Acceptor plasmid for even level position 3, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    lacZ
    

    Use

    Synthetic Biology

    Expression

    Plant

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge