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CRISPseq-BFP-backbone

Plasmid

#85707

Purpose

Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with a BFP fluorescent marker. Does NOT include Cas9.

Depositor

Ido Amit

Article

Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pmROSA26-attP50-Neo-mKate2-3xNLS-attP50

Plasmid

#183609

Purpose

Targeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Inserts

Neo
mKate2-3xNLS

Use

Mouse Targeting

Tags

3xNLS

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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p4B5-EGFP

Plasmid

#140324

Purpose

Detection of Dengue virus infection in live cells

Depositor

Alan Rothman

Article

Medin et al J Virol Methods. 2015 Jan;211:55-62. doi

Insert

DENV2-NGC NS4b5(10 a.a.)-NLS-eGFP

Tags

eGFP

Expression

Mammalian

Mutation

2 conservative n.t. differences near 5' end …

Promoter

CMV

Available Since

May 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMX-eSOX17FNV

Plasmid

#118838

Purpose

Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

Depositor

Ralf Jauch

Article

Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

Insert

eSOX17FNV (Sox17 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

FNV: L46F, V53N, E57V

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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Tbx21 gRNA#2

Plasmid

#170838

Purpose

Cas9-mediated knockout of Tbx21 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Tbx21 (Tbx21 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Tbx21 gRNA#3

Plasmid

#170839

Purpose

Cas9-mediated knockout of Tbx21 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Tbx21 (Tbx21 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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Tbx21 gRNA#1

Plasmid

#170837

Purpose

Cas9-mediated knockout of Tbx21 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Tbx21 (Tbx21 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCALNL-tdTomato

Plasmid

#125573

Purpose

Cre-dependent expression of tdTomato in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

tdTomato

Use

Cre/Lox

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-Mllt3

Plasmid

#131604

Purpose

expression of mouse MIIt3 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Mllt3 (Mllt3 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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LL - hOCT4i -1

Plasmid

#12198

Depositor

George Daley

Article

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

OCT4 - RNAi (POU5F1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pMXs-Lhx3

Plasmid

#32927

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Lhx3 (Lhx3 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
pMXs-Ngn2

Plasmid

#32928

Purpose

Retroviral expression of mouse Ngn2

Depositor

Kevin Eggan

Article

Son et al Cell Stem Cell. 2011 Sep 2;9(3):205-18.

Insert

Ngn2 (Neurog2 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only
FUW-TetO-Nanog

Plasmid

#40800

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Nanog (Nanog Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only
pDM358_DPAKa(GBD)-YFP

Plasmid

#128700

Purpose

expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

Depositor

Igor Weber

Article

Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

Insert

GBD domain (aa 731-890) of DPAKa

Use

Dictyostelium discoideum expression vector

Tags

YFP

Mutation

none

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pDM326_DPAKa(GBD)-YFP

Plasmid

#128701

Purpose

expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

Depositor

Igor Weber

Article

Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

Insert

GBD domain (aa 731-890) of DPAKa

Use

Dictyostelium discoideum expression vector

Tags

YFP

Mutation

none

Available Since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre

Plasmid

#125574

Purpose

Expresses Cre under the CAG promoter in mammalian cells.

Depositor

Takeshi Imai

Article

Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

Insert

Cre

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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CRISPseq-mCherry-backbone

Plasmid

#85708

Purpose

Backbone for CRISPR/Cas9 screening with single cell RNA-seq. Lentiviral plasmid for cloning of gRNAs and Unique Guide Index (UGI), with an mCherry fluorescent marker. Does NOT include Cas9.

Depositor

Ido Amit

Article

Jaitin et al Cell. 2016 Dec 15;167(7):1883-1896.e15.

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMT-Aurora B-GFP

Plasmid

#24285

Depositor

Ron Vale

Article

Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

Insert

Aurora B Kinase (aurB Fly)

Tags

GFP

Expression

Insect

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA6.MCV.LT339.V5.4

Plasmid

#28193

Depositor

Patrick Moore

Article

Shuda et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

Insert

MCPyV Truncated Tumor Large T antigen (399 tumor strain)

Tags

V5

Expression

Bacterial and Mammalian

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

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LL - hOCT4i -2

Plasmid

#12197

Depositor

George Daley

Article

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

OCT4 - RNAi (POU5F1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

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