Addgene

We narrowed to 60,832 results for: cell

Showing: 1781 - 1800 of 60832 results

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pCAG-GBP1-10gly-Gal4DBD

Plasmid

#49438

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP1-Gal4 DNA binding domain fusion protein

Use

Tags

Expression

Mammalian

Mutation

C98S (to increase protein solubility), Q3D

Promoter

CAG

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-Mllt3

Plasmid

#131604

Purpose

expression of mouse MIIt3 in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Mllt3 (Mllt3 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Sox2-IRES-Neo

Plasmid

#13462

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Sox2 (Sox2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 1-1290

Plasmid

#78579

Purpose

Expression flag-USP19 1-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 1-1290 (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

mutate 1291 residue to stop condon

Promoter

CMV

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pHAGE2-TetOminiCMV-Sox2

Plasmid

#136612

Purpose

Dox-inducible lentiviral vector expressing mouse Sox2

Depositor

Hans Schöler

Article

Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

Insert

Sox2 (Sox2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial

Mutation

Promoter

tetO-miniCMV (dox-inducible)

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD40L (lose of function mutant)-mgSrtA

Plasmid

#125796

Purpose

for co-expression of a loss of function mutant of CD40L and mgSrtA on cell membrane. A negative control to plasmid pcDNA3.1-CD40L-mgSrtA.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

CD40L (lose of function mutant)-mgSrtA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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LL - hOCT4i -2

Plasmid

#12197

Depositor

George Daley

Article

Zaehres et al Stem Cells. 2005 Mar . 23(3):299-305.

Insert

OCT4 - RNAi (POU5F1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2006

Availability

Academic Institutions and Nonprofits only

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Ef1a_NANOG_Ires_Puro

Plasmid

#18920

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

NANOG (NANOG Human)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pCI-neo-μ1A-(HA)3

Plasmid

#198177

Purpose

Expression of HA-tagged AP-1 μ1A in mammalian cells

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1A

Use

Tags

triple HA tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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CB6-GFP-TAOK1

Plasmid

#197108

Purpose

Expression of GFP-tagged TAOK1 / PSK2 in mammalian cells

Depositor

Jonathan Morris

Article

Garg et al J Cell Sci. 2020 Mar 16;133(6):jcs235895

Insert

TAOK1 (TAOK1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Wild Type

Promoter

CMV

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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mTiam1-mcherry-sspB in pcDNA3.1

Plasmid

#85221

Purpose

Role of Rac selective GEF domain from Tiam1 in immune cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Inserts

mTiam1 (1030-1406) (Tiam1 Mouse)
mcherry
sspB

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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Zyxin-GFP

Plasmid

#205095

Purpose

Express GFP-tagged human zyxin in mammalian cells

Depositor

Bianxiao Cui

Article

Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

Insert

zyxin (ZYX Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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MSCVpic2blast-EGFP-FF2

Plasmid

#164923

Purpose

Expresses EGFP cDNA and FF2 shRNA in mammalian cells

Depositor

Ji Luo

Article

Smith et al Cell Biosci. 2016 Nov 11;6:57. doi: 10.1

Inserts

Enhanced green fluorescent protein
Neuropeptide FF receptor 2 (NPFFR2 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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Mito-meCpG

Plasmid

#186462

Purpose

Expresses GFP tagged methyl-CpG recognition motif targeted to mitochondria in mammalian cells.

Depositor

Chongli Yuan

Article

Zhao et al ACS Sens. 2021 Oct 22;6(10):3575-3586. d

Insert

dimeric MBD1 (MBD1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pTV-Nanog Bgeo

Plasmid

#13837

Depositor

Shinya Yamanaka

Article

Mitsui et al Cell. 2003 May 30. 113(5):631-42.

Insert

IRES-Bgeo-pA cassette flanked by Nanog homologous regions (Nanog Mouse)

Use

Beta-gal

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
HS-DN-Ect2

Plasmid

#105956

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

Ect2 (ECT2 Human)

Use

Tags

EGFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pTV-Nanog Hyg

Plasmid

#13836

Depositor

Shinya Yamanaka

Article

Mitsui et al Cell. 2003 May 30. 113(5):631-42.

Insert

IRES-Hyg-pA cassette flanked by Nanog homologous regions (Nanog Mouse)

Use

Beta-gal

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pMX-eSOX17WHC

Plasmid

#118839

Purpose

Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

Depositor

Ralf Jauch

Article

Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

Insert

eSOX17WHC (Sox17 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

WHC: L46W, V53H, E57C

Promoter

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMX-eSOX17FNV

Plasmid

#118838

Purpose

Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

Depositor

Ralf Jauch

Article

Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

Insert

eSOX17FNV (Sox17 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

FNV: L46F, V53N, E57V

Promoter

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 1-493

Plasmid

#78581

Purpose

Expression flag-USP19 1-493 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 1-493 (USP19 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

mutated 494 residue to stop codon

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only