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RaCCR2_EYFP_pcDNA3.1

Plasmid

#159123

Purpose

This plasmid encodes RaCCR2 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RaCCR2

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA14-truncated_gb85_Cand9-trunc_lenti

Plasmid

#138312

Purpose

Lenti expression of AcrIIA14, C-terminus

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA14, C terminus

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEY31CBiT

Plasmid

#127761

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

Convergent LacI-TetR Toggle

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEYD1A

Plasmid

#127759

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach, MG RNA aptamer in divergent orientation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEYT1A

Plasmid

#127760

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach, MG RNA aptamer in tandem orientation

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA008

Plasmid

#128512

Purpose

Expresses Xylanase C

Depositor

Marcos Alcocer

Article

Furniss et al Biochim Biophys Acta. 2002 Jul 29;1598(1

Insert

Xylanase C

Expression

Bacterial

Promoter

Histone 4B

Available Since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBC32

Plasmid

#104697

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC32 (UBC32 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBC22

Plasmid

#104687

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC22 (UBC22 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBC26

Plasmid

#104691

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC26 (AT1G53025 Mustard Weed)

Use

Synthetic Biology

Mutation

BpiI/BsaI sites removed by point-mutation

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBC30

Plasmid

#104695

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC30 (UBC30 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBC11

Plasmid

#104676

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC11 (UBC11 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-UBC12

Plasmid

#104677

Purpose

Cloning Module

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

AtUBC12 (UBC12 Mustard Weed)

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-125

Plasmid

#104636

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB-F/A_EL4

Tags

HIS-Ubq, HA-E2, GST-E3

Expression

Bacterial

Mutation

AvrPtoB-Phe479Ala

Promoter

T7

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-412

Plasmid

#104638

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB_Myc-Fen_EL5

Tags

HIS-Ubq, HA-E2, GST-E3

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-413

Plasmid

#104639

Purpose

Level 2 construct

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Insert

HIS-Ubq_UBA1_HA-UBC8_GST-AvrPtoB-F/A_Myc-Fen_EL5

Tags

HIS-Ubq, HA-E2, GST-E3

Expression

Bacterial

Mutation

AvrPtoB-Phe479Ala

Promoter

T7

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pHL

Plasmid

#64171

Purpose

harbours marker genes HIS3 and LEU2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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PCx34.7IL-pET42

Plasmid

#42915

Depositor

John O'Brien

Article

Burr et al Biochem Biophys Res Commun. 2005 Oct 7;3

Insert

Connexin 34.7 cytoplasmic loop

Tags

6x His, GST, and S-tag

Expression

Bacterial

Mutation

cytoplasmic loop is aa 102-179

Promoter

T7

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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BS.YxiN.Twin1.a1-368.E152Q

Plasmid

#17517

Depositor

David McKay

Article

Wang et al Biophys J. 2008 Jan 1. 94(1):L01-3.

Insert

B. subtilis DEAD-box helicase YxiN first two domains

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

deleted amino acid residues 369-479; E152Q mutat…

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only
pEYmRD3A

Plasmid

#127756

Purpose

Research the effect of gene orientation of transcription

Depositor

Richard Murray

Article

Yeung et al Cell Syst. 2017 Jul 26;5(1):11-24.e12. d

Insert

mSpinach,RFP Divergent Orientation

Use

Synthetic Biology

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pEcgRNA

Plasmid

#166581

Purpose

CRISPR-Cas9-assisted genome editing in Escherichia coli

Depositor

Sheng Yang

Article

Li et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

ccdB

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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