We narrowed to 3,920 results for: pCMV mammalian

1. pCMV6-XL4 ASXL1 (1-479) 3x FLAG

   Plasmid

   #74262

   Purpose

   Cancer-associated ASXL1 truncation mutant in mammalian expression vector

   Depositor

   Anjana Rao

   Article

   Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

   Insert

   ASXL1 (ASXL1 Human)
   

   Use

   Tags

   3X FLAG

   Expression

   Mammalian

   Mutation

   Contains ASXL1 amino acids 1-479

   Promoter

   5’LTR

   Available since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pCMV-T7-SpCas9-NG-P2A-EGFP (RTW3677)

   Plasmid

   #139994

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. PCMV-intron myc Rab11 S25N

   Plasmid

   #46786

   Purpose

   expression of S25N Rab11a in mammalian cells

   Depositor

   Terry Hébert

   Article

   Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

   Insert

   RAB11A S25N (RAB11A Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   S25N dominant negative

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

   Plasmid

   #58414

   Purpose

   Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells

   Depositor

   Jacob Yount, Howard Hang

   Article

   Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

   Insert

   IFITM3 (Ifitm3 Mouse)
   

   Use

   Tags

   HA internal

   Expression

   Mammalian

   Mutation

   Cysteines 71, 72, and 105 to Alanine; The sequenc…

   Promoter

   CMV IE

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pCMV6-AN-HA_aa 321-843 REV1

   Plasmid

   #201587

   Purpose

   Expresses the catalytic domain (aa 321-843) of human DNA repair protein REV1 with a HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   DNA repair protein REV1 (REV1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Expresses amino acids 321-843 of DNA repair prote…

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV5D HA-L2D/V9D/I19D PPM1H

   Plasmid

   #207341

   Purpose

   Mammalian expression of HA-L2D/V9D/I19D PPM1H

   Depositor

   Suzanne Pfeffer

   Article

   Wondwossen M Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

   Insert

   PPM1H (PPM1H Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   L2D/V9D/I19D

   Promoter

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-TAPtagN-PrP-SBP-HsAGO2_AC

   Plasmid

   #148440

   Purpose

   Mammalian Expression of HsAGO2

   Depositor

   Elisa Izaurralde

   Article

   Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

   Insert

   HsAGO2 (AGO2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one silent mutation C840Tand sequence differs in …

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-PEmax-P2A-hP53DD-P2A-hMLH1dn

   Plasmid

   #214102

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and P2A-human MLH1dn

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PE-Plus
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLD037-pCMV-APOBEC-Cas9(D10A)-rXRCC1

   Plasmid

   #165444

   Purpose

   Expresses ACX, rXRCC1 in mammalian cells

   Depositor

   Wei Leong Chew

   Article

   Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

   Insert

   APOBEC-nCas9-rXRCC1 (Apobec1 Rat, Synthetic, Streptococcus pyogenes)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p212 pCMV-EGFP/RFP

    Plasmid

    #8389

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    floxed EGFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 31, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV5B-Flag-Smurf1 C699A

    Plasmid

    #11753

    Depositor

    Jeff Wrana

    Article

    Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

    Insert

    SMURF1 (SMURF1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C699A: catalytically inactive

    Promoter

    Available since

    May 12, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pCMV-GFP-MHC-IIB

    Plasmid

    #35691

    Depositor

    Venkaiah Betapudi

    Article

    Betapudi et al PLoS One. 2010 Jan 5;5(1):e8560.

    Insert

    MHC IIB (MYH10 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV Lex VP16 HA (P#1708)

    Plasmid

    #14593

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

    Insert

    LexA
    

    Use

    Tags

    HA and VP16

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pCMV6 Myc PAK5 K478M

    Plasmid

    #16020

    Depositor

    Jonathan Chernoff

    Article

    Cotteret et al Mol Cell Biol. 2003 Aug . 23(16):5526-39

    Insert

    PAK5 (PAK5 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K478M: kinase dead

    Promoter

    Available since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pCMV6 Myc PAK5 S573N

    Plasmid

    #16021

    Depositor

    Jonathan Chernoff

    Article

    Cotteret et al Mol Cell Biol. 2003 Aug . 23(16):5526-39

    Insert

    PAK5 (PAK5 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    S573N: constitutively active

    Promoter

    Available since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pCMV Ub Lex VP16 HA (#1709)

    Plasmid

    #14594

    Depositor

    Matija Peterlin

    Article

    Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

    Insert

    LexA
    

    Use

    Tags

    HA, Ubiquitin, and VP16

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

17. p259 pCMV-CRE-M (BglII)

    Plasmid

    #12493

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

    Insert

    CRE-M
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pCMV-Tag2B-E2F1

    Plasmid

    #202520

    Purpose

    Expressing human E2F1 protein with FLAG-tag

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Tags

    FLAG-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-CIB1-mCerulean-MP

    Plasmid

    #58366

    Purpose

    For use in light-induced protein inactivation through the interaction with CRY2

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Inserts

    CIB1[1-147] (CIB1 Mustard Weed)
    Camk2a[315-478] (Camk2a Rat)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOTTC590 - pCMV ER-iRFP

    Plasmid

    #59136

    Purpose

    A plasmid that express endoplasmic reticulum-targeted iRFP under the CMV promoter.

    Depositor

    Brandon Harvey

    Article

    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

    Insert

    ER-localized Infrared Fluorescent Protein
    

    Use

    Tags

    ER retention signal and signal peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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