We narrowed to 2,701 results for: LRE

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pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

Plasmid

#139090

Purpose

Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

Depositor

Neville Sanjana

Article

Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

Insert

dSpCas9-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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PGK mScarlet-LC3B

Plasmid

#200083

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

Map1lc3b (Map1lc3b Rat)

Tags

mScarlet

Expression

Mammalian

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pPBbsr-mKO-MK2

Plasmid

#115492

Purpose

Expression vector for the p38 activity reporter mKO-MK2 in mammalian cells

Depositor

Kazuhiro Aoki

Article

Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

Insert

mKO-MK2 (MAPKAPK2 Human)

Tags

monomeric Kusabira Orange (mKO)

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWZL-Blast FAPwt

Plasmid

#210643

Purpose

Expresses the FAP (Fibroblast Activation Protein) protein.

Depositor

Jing Yang

Article

Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

Insert

Fibroblast Activation Protein Alpha (FAP Human)

Use

Retroviral

Mutation

none

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_erGAP3

Plasmid

#78118

Purpose

Low affinity fluorescent calcium indicator of the GAP (GFP-Aequorin Protein) family targeted to the endoplasmic reticulum

Depositor

Teresa Alonso, Javier García-Sancho

Article

Navas-Navarro et al Cell Chem Biol. 2016 Jun 1. pii: S2451-9

Insert

erGAP3

Tags

KDEL and calreticulin signal peptide

Expression

Mammalian

Mutation

S175G, D180Y, I167V and L15Q in GFP moiety and D…

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-EGFPL10a

Plasmid

#98747

Purpose

Cre-dependently expresses the fusion protein EGFPL10a

Depositor

Nathaniel Heintz, Alexander Nectow, Eric Schmidt

Article

Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi

Has Service

AAV Retrograde and AAV5

Insert

EGFPL10a

Use

AAV and Cre/Lox

Tags

GFP

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAS_4xU6-PylT M15 (UUA) FLAG-Mma PylRS

Plasmid

#154774

Purpose

plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and Mma PylRS for ochre suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Mutation

in PylT: UUA anticodon and G10A, G14U, G16U, C20U…

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xG1 PylT FLAG-G1 PylRS Y125A

Plasmid

#154769

Purpose

plasmid with 4xG1 PylT cassette and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)

Tags

FLAG

Expression

Mammalian

Mutation

Y125A

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 shETV5-B

Plasmid

#74978

Purpose

shETV5 shRNA

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV5 (ETV5 Human)

Use

RNAi

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.5 shETV5-A

Plasmid

#74977

Purpose

shETV5 shRNA

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV5 (ETV5 Human)

Use

RNAi

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

Plasmid

#115787

Purpose

Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

FZD4 (FZD4 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gad65-WPRE-HGHpA

Plasmid

#184631

Purpose

Expresses Gad65, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gad65 (Gad2 Mouse)

Use

AAV

Promoter

EF1a

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 LRP5

Plasmid

#115907

Purpose

Expresses human LRP5 in mammalian cells.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

LRP5 (LRP5 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pLX_TRC311 ETV4-L

Plasmid

#74982

Purpose

Constitutive ETV4 ORF, blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV4 (ETV4 Human)

Expression

Mammalian

Mutation

S396N

Promoter

EF1a

Available Since

July 7, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-TORCAR

Plasmid

#64929

Purpose

Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

Depositor

Jin Zhang

Article

Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

Insert

LAMP1-TORCAR (LAMP1 Human, Synthetic)

Tags

Cerulean, Full-length LAMP1, and YPet

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Con/Fon TVA-mCherry

Plasmid

#131779

Purpose

INTRSECT 2.0

Depositor

Karl Deisseroth

Article

Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

Insert

TVA-mCherry

Use

AAV; Intrsect

Tags

mCherry

Expression

Mammalian

Promoter

nEF

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pNTI194

Plasmid

#84266

Purpose

Expresses Avi-tagged RPL1 and humanized birA

Depositor

Nicholas Ingolia

Article

Ingolia et al Cell Rep. 2014 Sep 11;8(5):1365-79. doi:

Inserts

RPL1 (RPL10A (*see below), Human)
humanized birA

Tags

Avi tag, Flag, Gly/Ser linker, and TEV site

Expression

Mammalian

Mutation

R33A, A34E (*see below)

Promoter

CMV and P2A peptide

Available Since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO- FOS

Plasmid

#125598

Purpose

doxycycline-inducible expression of human FOS in mammalian cells

Depositor

Filipe Pereira

Article

Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

Insert

Fos proto-oncogene, AP-1 transcription factor subunit (FOS Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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CMV mScarlet-LC3B

Plasmid

#200431

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

Map1lc3b (Map1lc3b Rat)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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LV-hHOXA13

Plasmid

#135561

Purpose

Express hHOXA13 and EGFP in mammalian cells

Depositor

Jose Teixeira

Article

George et al Cell Rep. 2019 Dec 17;29(12):4069-4085.e

Insert

Homeobox A13 (HOXA13 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CBH

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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