We narrowed to 13,486 results for: cas9 genes

1. pU6-(BsaI)_CBh-UN-Cas9

   Plasmid

   #135011

   Purpose

   Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9

   Depositor

   Yosef Shaul

   Article

   Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio

   Insert

   humanized S. pyogenes Cas9
   

   Use

   Tags

   126aa domain from HSV-1 UL12 fused to the N-termi…

   Expression

   Mammalian

   Mutation

   126aa domain from HSV-1 UL12 fused to the N-termi…

   Promoter

   CBh

   Available since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. dCas9-SunTag(10X)-2A-BFP

   Plasmid

   #227389

   Purpose

   Adhesion module, dCas9-SunTag

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   dCas9-SunTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Enzymatically dead Cas9

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3xFLAG-dCas9/pTEF1p-CYC1t

   Plasmid

   #62190

   Purpose

   Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Yeast

   Mutation

   human codon-optimized, D10A, H840A

   Promoter

   TEF1 promoter

   Available since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

   Plasmid

   #163572

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-NG-nCas9-UdgX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

   Promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-FLEXfrt-SaCas9-U6-sgGria1

   Plasmid

   #124867

   Purpose

   Mutagenesis of Gria1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria1 (Gria1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-tight-DDdCas9GFP-IRES-Neo

   Plasmid

   #102907

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

   Plasmid

   #118842

   Purpose

   Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   cdkn2a gRNA (LOC100329528 Zebrafish)
   

   Use

   CRISPR; Tol2

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-CMV-NLS-dCas9-NLS

   Plasmid

   #99908

   Purpose

   For the expression of NLS-dCas9-NLS

   Depositor

   Yujie Sun

   Article

   Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

   Insert

   NLS-dCas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-xCas9RA-P2A-Puro

   Plasmid

   #110871

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)RA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-FLEX-SaCas9-U6-sgSlc17a7

    Plasmid

    #124846

    Purpose

    Mutagenesis of Slc17a7

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a7 (Slc17a7 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEXfrt-SaCas9-U6-sgGria2

    Plasmid

    #124868

    Purpose

    Mutagenesis of Gria2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria2 (Gria2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-hIP-dCas9-BSD

    Plasmid

    #183231

    Purpose

    Lentiviral vectors with the human insulin promoter driving expression of dCas9, in addition to a U6-driven sgRNA

    Depositor

    Jorge Ferrer

    Article

    Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Human insulin promoter

    Available since

    Oct. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-hCas9-T2A-mStrawberry

    Plasmid

    #187224

    Purpose

    Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Inserted T2A-mStrawberry at 3' terminal of M…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6

    Plasmid

    #124860

    Purpose

    Mutagenesis of Slc17a6

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a6 (Slc17a6 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX429 SpCas9 N863A nickase

    Plasmid

    #68706

    Purpose

    SpCas9 N863A nickase

    Depositor

    Feng Zhang

    Article

    Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

    Insert

    SpCas9 N863A nicking mutant
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CMV-SauriCas9-Puro

    Plasmid

    #135965

    Purpose

    Expresses SauriCas9 and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9-CRY2Clust-FUSN-mut

    Plasmid

    #200970

    Purpose

    induction of phase-separated condensate mediated by FUSN-IDR mutant

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    FUSNmut
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    15 Tyr-to-Ser substitutions

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX551-miniCMV-SpCas9D10A nickase

    Plasmid

    #216735

    Purpose

    Expresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)

    Depositor

    Vincent Dion

    Article

    Alvaro et al bioRxiv

    Insert

    Cas9-D10A nickase
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    T7 and mini-CMV

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. dCas9-NLS-gs10-cLYN268

    Plasmid

    #188259

    Purpose

    Plasmid ensures constitutive expression of the C-terminal fragment of split LYN kinase domain (aa 239-267), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    cLYN268 (LYN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas9-NLS-gs10-cERb447

    Plasmid

    #188257

    Purpose

    Plasmid ensures constitutive expression of the C-terminal fragment of split ERbeta (aa 447-501), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus

    Depositor

    Roman Jerala

    Article

    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

    Insert

    cERb447 (ESR1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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