We narrowed to 4,373 results for: p2a

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Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

Plasmid

#208048

Purpose

Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

BirAopt

Use

Lentiviral

Mutation

BirAopt is human-codon-optimized BirA (PMID 15707…

Promoter

EFS

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLY017SB_pAAV-U6sg(BbsI)-EFS-Thy1.1-P2A-SB100X

Plasmid

#192151

Purpose

T cell CRISPR AAV-SB vector

Depositor

Sidi Chen

Article

Ye et al Nat Biotechnol. 2019 Nov;37(11):1302-131

Type

Empty backbone

Use

AAV

Expression

Mammalian

Mutation

NA

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EWB-DIO-myriRFP670V5-P2A-post-eGRASP

Plasmid

#111585

Purpose

An AAV vector that expresses double floxed myristoylated iRFP670 with V5 tag and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myriRFP670V5-P2A-post-eGRASP

Use

AAV

Promoter

Ef1a

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pHR-BFP-NLS-P2A-RSV P-1xALFATag@72AA

Plasmid

#246864

Purpose

Express BFP-NLS-P2A-RSV P-1xALFATag@72AA (Pexo-ALFATag) for RSV imaging.

Depositor

Marvin Tanenbaum

Article

Ratnayake et al Nature. 2026 Jan 28. doi: 10.1038/s41586

Insert

BFP-NLS-P2A-RSV P-1xALFATag@72AA

Use

Lentiviral

Tags

BFP-NLS

Expression

Mammalian

Mutation

RSV phosphoprotein (P) contains a single ALFA Tag…

Promoter

SFFV

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a eIL15 IRES mNeonGreen-P2A-Puro

Plasmid

#248036

Purpose

Constitutively expresses an engineered version of IL15, mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Insert

Engineered IL15

Use

Lentiviral

Expression

Mammalian

Promoter

human EF1a

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

Plasmid

#225756

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMJA289= pSin-EF2-TRAV10-P2A-TRBV25-Puro

Plasmid

#116876

Purpose

stable TCR expression in human T cells

Depositor

Marcos Alcocer

Article

Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

Insert

TRAV10 - P2A - TRBV25 (TRAV10, TRBV25-1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-alpha

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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TRE3G-ZIM3-Cas9-P2A-GFP-PGK-Blasti

Plasmid

#239604

Purpose

Doxycycline inducible CRISPRgenee construct with a blasticidin resistance

Depositor

Philipp Rathert

Article

Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

Insert

ZIM3 KRAB domain (ZIM3 )

Use

CRISPR and Lentiviral

Promoter

TRE3G

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2-17

Plasmid

#210020

Purpose

tetO-OKS* (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + super-Sox)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Oct4-p2a-Klf4-IRES-Sox2-17 (Klf4, Pou5f1, Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO-mini-CMV

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:ECFP

Plasmid

#158654

Purpose

making transgenics expressing a QUAS driven YFP and NTR 2.0

Depositor

Jeff Mumm

Article

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xQUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:eCFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8e-SpRY-HF1-P2A-EGFP (LM434)

Plasmid

#197507

Purpose

CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with nSpRY-HF1(D10A) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

pCMV-T7-BPNLS-ABE8e-nSpRY-HF1-BPNLS-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-P2A-EGFP

Expression

Mammalian

Mutation

ABE8e mutations in TadA and nSpRY-HF1(D10A/A61R/N…

Promoter

CMV and T7

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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MF87_PB-EF1a-TET3G-P2A-ERT2-Gal4-BGHpA

Plasmid

#176630

Purpose

Integrated in CHO-K1 to make parental polyclonal lines for all other MultiFate cell lines

Depositor

Michael Elowitz

Article

Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

Insert

EF1a-TET3G-P2A-ERT2-Gal4-BGHpA

Use

Synthetic Biology

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EWB-DIO-myrTagRFP-T-P2A-post-eGRASP

Plasmid

#111581

Purpose

An AAV vector that expresses double floxed myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myrTagRFP-T-P2A-post-eGRASP

Use

AAV

Promoter

Ef1a

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP

Plasmid

#221233

Purpose

Express sgRNA targeting HEK3 loci with nCas9(D10A)

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

SpCas9(D10A)-P2A-EGFP

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST

Plasmid

#208038

Purpose

Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

Insert

PML (isoform IVa) (PML Human)

Use

Lentiviral

Tags

FLAG, N-terminal Split-TurboID

Promoter

EFS

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-EFS-BirAopt-GSQ-RNF4wt-P2A-BLAST

Plasmid

#208046

Purpose

Enables constitutive expression of N-terminal BirAopt-fused RNF4wt; to perform bioE3; selection with blasticidin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

RNF4 (RNF4 Human)

Use

Lentiviral

Tags

BirAopt

Promoter

EFS

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE

Plasmid

#131004

Purpose

bicistronic AAV vector to express GCaMP6m and soma-targeted ChRmine under the control of human Synapsin promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

ChRmine

Use

AAV

Tags

Kv2.1-HA

Promoter

hSyn

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pHIV-NAT-FLAG-CIP2A ∆NES (del561-625)

Plasmid

#222625

Purpose

Lentiviral vector that expresses Flag-tagged CIP2A NES mutant in mammalian cells

Depositor

Daniel Durocher

Article

Adam et al Nat Cancer. 2021 Dec;2(12):1357-1371. do

Insert

CIP2A (CIP2A Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Deletion of the residues 561-625 (ΔNES)

Promoter

EF-1-alpha promoter

Available Since

July 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP

Plasmid

#164038

Purpose

Expression of dCas9-14xGFP11 in mammalian cells

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

dCas9-14xGFP11

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

dSV40

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX

Plasmid

#202606

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P-P2A-mCherry-CAAX

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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