159,557 results

1. pLentiPGK Puro DEST p38KTRClover

   Plasmid

   #59152

   Purpose

   Lentiviral vector to express p38 KTR mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-dCas9-VP64

   Plasmid

   #47107

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   Flag, HA, SV40 NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-FLEX-P301L Tau

   Plasmid

   #176267

   Purpose

   This AAV plasmid vector expresses human 2N4R microtubule-associated protein tau with P301L mutation.

   Depositor

   Tsuneya Ikezu

   Article

   You et al Neuroscience. 2019 Dec 1;422:65-74. doi:

   Insert

   Flex-P301L tau (MAPT )
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-OTp-GCaMP6s

   Plasmid

   #192945

   Purpose

   To drive GCaMP6s under the control of mouse oxytocin promoter

   Depositor

   Kazunari Miyamichi

   Article

   Yaguchi et al PLoS One. 2023 May 10;18(5):e0285589. do

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

   Plasmid

   #201916

   Purpose

   Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Inserts

   Gag-Cas9 v2
   B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and Human U6

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. DRD2-DuET

   Plasmid

   #213228

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   DRD2 (DRD2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2-Opto-zFGFR1a

   Plasmid

   #232639

   Purpose

   Zebrafish FGFR1a receptor kinase domain fused to VfLOV

   Depositor

   Katherine Rogers

   Insert

   zFGFR1a kinase domain + VfLOV domain
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry-GFAP-N-18

   Plasmid

   #55051

   Purpose

   Localization: Intermediate Filaments, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   GFAP (GFAP Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pGEX4T3-GST-Tev

   Plasmid

   #177142

   Purpose

   Bacterial vector for expression of an N-terminal GST fusion of the Tev protease

   Depositor

   Robert Sobol

   Article

   Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

   Insert

   Tev
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Tac

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pScaffold-H1 donor

    Plasmid

    #118152

    Purpose

    PCR template for dual guide RNA cloning, guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system - H1 promoter

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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