We narrowed to 11,928 results for: pcDNA

1. pcDNA3.1 Mfn2-HA S27A

   Plasmid

   #139193

   Purpose

   Expresses C-terminal HA tagged human Mfn2 S27A in mammalian cells

   Depositor

   Allan Weissman

   Article

   Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

   Insert

   MFN2 (MFN2 Human)
   

   Use

   Tags

   3HA

   Expression

   Mammalian

   Mutation

   Serine 27 to Alanine

   Promoter

   CMV promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-flag-YTHDF2

   Plasmid

   #52300

   Purpose

   full length YTHDF2 with N-terminal flag tag for mammalian cell expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTHDF2 (YTHDF2 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-ALK-V5/HIS

   Plasmid

   #200115

   Purpose

   Expression of human ALK receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human ALK receptor tyrosine kinase (ALK Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-N-Flag-ASC1

   Plasmid

   #75134

   Purpose

   Expresses N-terminal Flag-tagged ASC1 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   PYD and CARD domain containing (Pycard Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F23S (please see depositor comments below)

   Promoter

   CMV

   Available since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-TRKB-V5/HIS

   Plasmid

   #205614

   Purpose

   Expression of human TRKB receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   TRKB receptor tyrosine kinase (NTRK2 Human)
   

   Use

   Tags

   V5-His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 Mfn2-HA S27D

   Plasmid

   #139194

   Purpose

   Expresses C-terminal HA tagged human Mfn2 S27D in mammalian cells

   Depositor

   Allan Weissman

   Article

   Leboucher et al Mol Cell. 2012 Aug 24;47(4):547-57. doi:

   Insert

   MFN2 (MFN2 Human)
   

   Use

   Tags

   2HA

   Expression

   Mammalian

   Mutation

   Serine 27 to Aspartic Acid

   Promoter

   CMV promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-EGFP-Rac1(wt)

   Plasmid

   #13719

   Depositor

   Klaus Hahn

   Article

   Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

   Insert

   EGFP-Rac1(wt) (RAC1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-ALK5 T204D

   Plasmid

   #80877

   Purpose

   mammalian expression of constitutively active ALK5 T204D

   Depositor

   Aristidis Moustakas

   Article

   Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

   Insert

   ALK5 (TGFBR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   T204D (constitutively active)

   Promoter

   CMV

   Available since

   Aug. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-FLAG-Rheb

   Plasmid

   #19996

   Depositor

   Fuyuhiko Tamanoi

   Article

   Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   Ras homolog enriched in brain 1 (RHEB Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-ERBB2-V5/HIS

    Plasmid

    #201103

    Purpose

    expression of human ERBB2 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human ERBB2 receptor tyrosine kinase, full length, wildtype (ERBB2 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mtsCOXVIIIx2-B-gTEMP/pcDNA3

    Plasmid

    #199806

    Purpose

    Fluorescent temperature indicator B-gTEMP fused with mitochondrial targeting sequence

    Depositor

    Takeharu Nagai

    Article

    Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

    Insert

    B-gTEMP
    

    Use

    Tags

    mtsCOXVIIIx2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-PBZ-mRuby2

    Plasmid

    #110650

    Purpose

    expresses a PAR-binding (PBZ) domain fused to mRuby2

    Depositor

    Chris Lord

    Article

    Krastev et al Nat Commun. 2018 May 22;9(1):2016. doi:

    Insert

    PBZ domain from APLF, amino acids 371-451 (APLF Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA-mito-GreenPy1Highest

    Plasmid

    #239069

    Purpose

    Mammalian expression of mitochondria-targeted highest affinity green fluorescent pyruvate biosensor GreenPy1Highest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    GreenPy1Highest
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA-mito-ApplePy1Highest

    Plasmid

    #239074

    Purpose

    Mammalian expression of mitochondria-targeted highest affinity red fluorescent pyruvate biosensor ApplePy1Highest

    Depositor

    Robert Campbell

    Article

    Imai et al bioRxiv 2025.04.17.649293

    Insert

    ApplePy1Highest
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCDNA5 TEX264wt-cGFP

    Plasmid

    #220211

    Purpose

    Mammalian expression of wild type TEX264 fused at C-terminus to GFP

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-I-PhoCl-HCVp

    Plasmid

    #87697

    Purpose

    Express I-PhoCl-HCVp with N-term EBFP2 in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    I-PhoCl-HCVp containing the Ki = 10.5 nM inhibitor (CP5-46A-4D5E)
    

    Use

    Tags

    EBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1-DDR1-V5/HIS

    Plasmid

    #201100

    Purpose

    expression of human DDR1 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human DDR1 receptor tyrosine kinase, full length, wildtype (DDR1 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3/hArf6(Q67L)-HA

    Plasmid

    #79425

    Purpose

    Expresses C-terminally HA-tagged Arf6(Q67L) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamine 67 to Leucine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3/hArf6(T27N)-HA

    Plasmid

    #79426

    Purpose

    Expresses C-terminally HA-tagged Arf6(T27N) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Threonine 27 to Asparagine

    Promoter

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5FRT/Flag-KIAA1267

    Plasmid

    #29758

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    KIAA1267 (KANSL1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge