We narrowed to 18,831 results for: Tec

1. pDiySpike2

   Plasmid

   #136949

   Purpose

   Plasmid for the In Vitro transcription of DiySpike2

   Depositor

   Rickard Sandberg

   Article

   Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

   Insert

   DiySpike2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available Since

   April 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBC2102-reci

   Plasmid

   #117171

   Purpose

   U6-sgRNA-EFS-Cas9-T2A-mCherry-P2A-Hygro; wtCas9 without NLS

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   reci_wtCas9 without NLS
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6 Promoter

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBW810_pCAG-PV7-loxN-F14-mCherry-BGHpA

   Plasmid

   #87586

   Purpose

   PV7 for 3-input, 2-output Full Adder

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   mCherry
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCDF-yibDp-matB

   Plasmid

   #134597

   Purpose

   Low Phosphate inducible matB (malonyl-CoA synthetase)

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   Malonyl CoA synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   yibD

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCfB2198

   Plasmid

   #67554

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCellFree_G03 GTF2H2D

   Plasmid

   #67081

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   GTF2H2D (GTF2H2C_2 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pRS416 Gal-RNQ1 1-153-YFP

   Plasmid

   #18690

   Depositor

   Susan Lindquist

   Article

   Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

   Insert

   Rnq1 1-153
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   NonPrion domain of Rnq1

   Promoter

   Available Since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pCfB2228

   Plasmid

   #67540

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pX330A_FokI-1x6

   Plasmid

   #63606

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDM_noro_A1_lacZA

    Plasmid

    #118818

    Purpose

    T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A1) with a lacZ alpha subunit reporter.

    Depositor

    Alexander Green

    Article

    Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

    Insert

    Norovirus toehold switch A1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNH078

    Plasmid

    #42164

    Depositor

    Colin Dolphin

    Article

    Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

    Insert

    mTFP1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Feb. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TAL3144

    Plasmid

    #41318

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-pum1-Left (pum1 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

13. TAL3145

    Plasmid

    #41319

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-pum1-Right (pum1 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pMAD-Gate3

    Plasmid

    #25942

    Depositor

    Kamil Onder

    Article

    Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

    Insert

    
    

    Use

    Tags

    VP16 Fusion Protein

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMBD-Gate3

    Plasmid

    #25959

    Depositor

    Kamil Onder

    Article

    Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

    Insert

    
    

    Use

    Tags

    GAL4 Fusion Protein

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCellFree_G03 RAP2A

    Plasmid

    #67058

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. TAL3200

    Plasmid

    #41374

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-vascular-endothelia-growth-factor-aa-(vegfaa)-Left
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

18. TAL3201

    Plasmid

    #41375

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-vascular-endothelia-growth-factor-aa-(vegfaa)-Right
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

19. CD4_LD 0_TEV

    Plasmid

    #58870

    Purpose

    MESA protease chain with CD4 ectodomain and no linker domain

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA protease chain with CD4 ectodomain and TEV protease
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TAL2219

    Plasmid

    #36678

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    MUTYH-TALEN_Right (Mutyh Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pPZP FLIPmal-25u

    Plasmid

    #14476

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPmal
    

    Use

    Plant nanosensor

    Tags

    ECFP and EYFP

    Expression

    Plant

    Mutation

    W230A

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

22. pBSKΔB-CBh-Cas9n-pA

    Plasmid

    #82617

    Purpose

    Expresses Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

    Insert

    SpCas9n
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAWp7-10

    Plasmid

    #72742

    Purpose

    pFUGW-UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]

    Depositor

    Timothy Lu

    Article

    Wong et al Nat Biotechnol. 2015 Sep;33(9):952-61. d

    Insert

    UBCp-RFP-[miR-124 sensor]-CMVp-GFP-[miR-124]
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Opie2-PuroR[traF]

    Plasmid

    #131615

    Purpose

    Expresses a functional PuroR only in females and dsRed in both genders.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Insert

    PuroR[traF]
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Opie2

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET11a+D77R/T214V

    Plasmid

    #66135

    Purpose

    Expresses the D77R/T214V mutant of CYP119

    Depositor

    Teruyuki Nagamune

    Article

    Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

    Insert

    CYP119 (cyp119 )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D77R, T214V

    Promoter

    T7 promoter

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. TAL2246

    Plasmid

    #36691

    Depositor

    Keith Joung

    Article

    Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

    Insert

    PHF8-TALEN_Left (Phf8 Human)
    

    Use

    Mammalian, t7 for in vitro mrna synthesis

    Tags

    3X Flag and WT FOKI

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pBW708_pCAG-PV4-loxP-GFP-T2A-mCherry

    Plasmid

    #89057

    Purpose

    PV4 for 2-input, 2-output circuit library

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    GFP-T2A-mCherry
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBLIC-puro

    Plasmid

    #45197

    Purpose

    a ligation-independent cloning (LIC) retroviral vector for stable gene transduction in mammalian cells. Puro selection.

    Depositor

    Priyamvada Rai

    Article

    Patel et al BMC Biotechnol. 2012 Jan 16;12:3. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

29. TAL3175

    Plasmid

    #41349

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-socs3b-Right (socs3b Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

30. V207 pRETRO-Triple-Flag SD - Acceptor

    Plasmid

    #11709

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

    Type

    Empty backbone

    Use

    Cre/Lox and Retroviral; Acceptor vector

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. TAL3250

    Plasmid

    #42713

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-esr1-left (esr1 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

32. TAL3251

    Plasmid

    #42714

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-esr1-right (esr1 Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

33. TAL3366

    Plasmid

    #42829

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-sox1a-left (sox1a Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

34. TAL3367

    Plasmid

    #42830

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-sox1a-right (sox1a Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

35. pBW768_pCAG-PV1-FRT-lox2272-BFP-BGHpA

    Plasmid

    #87574

    Purpose

    PV1 for 2-input, 4-output decoder circuit

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    mtagBFP
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. TAL3124

    Plasmid

    #41298

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-mycn-Left (mycn Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

37. TAL3125

    Plasmid

    #41299

    Depositor

    Keith Joung

    Article

    Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

    Insert

    ZebrafishCommunity-mycn-Right (mycn Zebrafish)
    

    Use

    t7

    Tags

    3X Flag and WT FOKI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

38. pBT195

    Plasmid

    #127843

    Purpose

    clone BE4max CBEs by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    BE4max without deaminase
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pX330A_FokI-1x3

    Plasmid

    #63603

    Purpose

    Expresses FokI-dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    FokI-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCY 3050-07

    Plasmid

    #36223

    Depositor

    Anne Robinson

    Article

    Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

    Insert

    Streptoalloteichus hindustanus bleomycin (Sh ble)
    

    Use

    Yeast cassette for pcr and homologous recombinati…

    Tags

    yEpolylinker-Venus-Zeocin

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge