We narrowed to 4,184 results for: 256

1. GfaABC1D BARK D110A p2A mCherry

   Plasmid

   #117692

   Purpose

   GfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs D110A peptide
   

   Use

   AAV

   Tags

   HA / FLAG

   Expression

   Mutation

   D110A

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMaRSC

   Plasmid

   #89189

   Purpose

   The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.

   Depositor

   David Tirrell

   Article

   Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

   Insert

   Flag-L274G-T2A-mCherry (Mars1 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG FLEX BARK D110A p2A mCherry

   Plasmid

   #117694

   Purpose

   CAG-FLEx-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for Cre-dependent Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs D110A peptide
   

   Use

   Cre/Lox

   Tags

   HA / FLAG

   Expression

   Mutation

   D110A

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA GNSTM-3-Flag-10-Lamp2b-HA

   Plasmid

   #71293

   Purpose

   Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Use

   Tags

   FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2+8 Flag2AUF1(p45)

   Plasmid

   #135947

   Purpose

   Expresses AUF1p45 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

   Depositor

   Yimon Aye

   Article

   Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

   Insert

   heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
   

   Use

   Tags

   Flag2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and SP6

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-FKBP-PIP4K2B

   Plasmid

   #202734

   Purpose

   expresses recruitable fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   mCherry:FKBP1A (3-108):[GGSA]4GG:PIP4K2B (PIP4K2B, FKBP1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA Flag Lamp2b-HA

   Plasmid

   #71292

   Purpose

   Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Use

   Tags

   Flag, HA, and Lamp2 signal peptide

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. STARR-seq luciferase validation vector_ORI_neg.cont

   Plasmid

   #99315

   Purpose

   Negative control for luciferase validation vectors

   Depositor

   Alexander Stark

   Article

   Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

   Insert

   neutral region; chr3: 55198740-55200041
   

   Use

   Luciferase; Starr-seq luciferase validation vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-myc-HLA-A24

   Plasmid

   #135507

   Purpose

   Mammalian expression of myc-tagged HLA-A*024:02

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*24:02 (HLA-A Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1-hnRNPU-V5

    Plasmid

    #35974

    Depositor

    Susana Valente

    Article

    Mediouni et al Curr HIV Res. 2015;13(1):64-79.

    Insert

    heterogeneous nuclear ribonucleoprotein U (HNRNPU Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTol2pA2-ubb:secHsa.ANNEXINV-mVenus, cryaa:RFP

    Plasmid

    #92388

    Purpose

    Cell death reporter

    Depositor

    Nicholas Cole

    Article

    Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

    Insert

    ANNEXINV (ANXA5 Human)
    

    Use

    Zebrafish expression

    Tags

    mVenus

    Expression

    Mutation

    Promoter

    ubb

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEX-SaCas9-U6-sgGabrg2

    Plasmid

    #124857

    Purpose

    Mutagenesis of Gabrg2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gabrg2 (Gabrg2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Pseudojanin (PJ)

    Plasmid

    #37999

    Depositor

    Robin Irvine

    Article

    Hammond et al Science. 2012 Jun 21.

    Inserts

    FKBP1A (FKBP1A Human)
    SAC1
    INPP5E (INPP5E Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2+8 Flag2AUF1(p37)

    Plasmid

    #135944

    Purpose

    Expresses AUF1p37 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Use

    Tags

    Flag2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MC4R

    Plasmid

    #233630

    Purpose

    encodes the human melanocortin 4 receptor, without any tags

    Depositor

    Christian Gruber

    Article

    Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

    Insert

    MC4R (MC4R Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/T7

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSBtet rtTA G72V SE Δsplice GP Luc

    Plasmid

    #194328

    Purpose

    Tetracycline inducible expression of Luciferase enzyme in mammalian cells with inclusion of rtTA G72V mutation, splice site and high sensitivity mutations for increased promotor tightness

    Depositor

    Alexander McLellan

    Article

    Saunderson et al Genes (Basel). 2022 Sep 20;13(10). pii:

    Insert

    Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Tet-responsive promotor related mutations: G72V i…

    Promoter

    Tight tet-responsive promotor

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. RANLS

    Plasmid

    #50843

    Purpose

    Expresses one ddFP in mammalian cells, the second plasmid for a green to red colour switch-based fluorescent caspase-3 biosensor, used together with plasmid GANES-DEVD-BNLS.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A
    

    Use

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GANLS

    Plasmid

    #50852

    Purpose

    Expresses one ddFP in mammalian cells, the second plasmid for a red to green colour switch-based fluorescent caspase-3 biosensor, used together with plasmid NESRA-DEVD-BNLS.

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A
    

    Use

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. single polypeptide FPX biosensor for calcium ion

    Plasmid

    #60887

    Purpose

    FPX biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for calcium ion
    

    Use

    Tags

    HindIII site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. EE-LRRK2

    Plasmid

    #193583

    Purpose

    Expresses full-length LRRK2 with FYVE-CC2 domains from HRS and 3xFLAG on the N-terminus

    Depositor

    Mark Cookson

    Article

    Kluss et al Proc Natl Acad Sci U S A. 2022 Oct 25;11

    Insert

    Leucine-rich repeat kinase 2 (LRRK2 Human)
    

    Use

    Tags

    FYVE-CC2 and 3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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