We narrowed to 2,963 results for: PHI-1;

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mMib1-FLAG

Plasmid

#37116

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

Mib1(Mus musculus mindbomb homolog 1 (Drosophila) (Mib1)) (Mib1 Mouse)

Tags

3 x FLAG

Expression

Mammalian

Mutation

ACC Kozak sequence added before ATG annotated.

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pMUH_unc84_tdTFlag

Plasmid

#46024

Depositor

Sean Eddy

Article

Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

Insert

UNC-84 (unc-84 )

Use

10xuas-phi31-expression vector

Tags

tdTomato

Mutation

Two amino acid mutations (F279L and L500P) compar…

Promoter

10XUAS

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pMUH_unc84_2XGFP

Plasmid

#46023

Depositor

Sean Eddy

Article

Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

Insert

UNC-84 (unc-84 )

Use

10xuas-phi31-expression vector

Tags

2XGFP

Mutation

Two amino acid mutations (F279L and L500P) compar…

Promoter

10XUAS

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pBS-GMR-eya(shRNA)

Plasmid

#157991

Purpose

pBSII-KS(-)-based vector backbone containing a dominant eye marker for constructing Drosophila CRISPR HDR templates. Marker allows counter selection against vector integration events.

Depositor

Greg Beitel

Article

Nyberg et al Fly (Austin). 2020 Oct 5. doi: 10.1080/1

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJFRC7-UAS-NPRR_dTK

Plasmid

#133244

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

drosophila tachykinin-fused codon-optimized GCaMP6s

Expression

Insect

Promoter

hsp70

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAWH-Rluc-let-7-A114-N40-HhR

Plasmid

#50555

Purpose

Expression of luciferase reporter mRNA in S2 cell

Depositor

Yukihide Tomari

Article

Fukaya et al Mol Cell. 2012 Dec 28;48(6):825-36. doi:

Insert

Luciferase-let7-A114-N40-HhR

Use

Luciferase

Expression

Insect

Promoter

Actin5C

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pAWH-Rluc-let-7-Poly(A)

Plasmid

#50556

Purpose

Expression of luciferase reporter mRNA in S2 cell

Depositor

Yukihide Tomari

Article

Fukaya et al Mol Cell. 2012 Dec 28;48(6):825-36. doi:

Insert

Luciferase-let7-Poly(A)

Use

Luciferase

Expression

Insect

Promoter

Actin5C

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmHPat

Plasmid

#25029

Depositor

Elisa Izaurralde

Article

Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

Insert

HPat (Patr-1 Fly)

Use

Drosophila expression

Tags

EGFP

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1_1147-1386-3xL-Amut_P

Plasmid

#147167

Purpose

Insect Expression of DmNot1_1147-1386-3xL-Amut

Depositor

Elisa Izaurralde

Insert

DmNot1_1147-1386-3xL-Amut (Not1 Fly)

Expression

Insect

Mutation

1 silent mutation compared to the sequence given …

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pT2iU6Apowriter2N(#355)

Plasmid

#184106

Purpose

cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis

Depositor

Sophie Vriz

Article

Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

Insert

myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2

Use

Zebrafish transient transgenesis + in vitro trans…

Tags

internal tag: 5myc

Mutation

DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

Promoter

Zf-Ubi+SP6

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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C-term png in pGEX4

Plasmid

#112975

Purpose

For protein expression and purification of the C-term of Drosophila png

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png (png Fly)

Expression

Bacterial

Mutation

wild-type C terminus

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pJP118

Plasmid

#176494

Purpose

Expresses Lifeact-mScarlet under control of the Capsaspora EF1 promoter and HygR under control of the Capsaspora actin promoter

Depositor

Duojia Pan

Article

Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

Insert

Lifeact peptide

Use

Capsaspora owczarzaki expression

Available Since

June 6, 2022

Availability

Academic Institutions and Nonprofits only

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pME18ST-d5-HT2A

Plasmid

#53109

Purpose

Expression of Drosophila 5-HT2A

Depositor

Leslie Vosshall

Article

Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

Insert

5HT2A (5-HT2 Fly)

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
C2 png genomic in Casper

Plasmid

#112971

Purpose

P element vector for transposon insertion of png genomic DNA into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png genomic DNA (png Fly)

Use

P insertion vector

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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C2 M2 png-myc in Casper4

Plasmid

#112968

Purpose

P element vector for transposon insertion of Myc-tagged png into Drosophila genome

Depositor

Terry Orr-Weaver

Article

Fenger et al Development. 2000 Nov;127(22):4763-74.

Insert

png-myc (png Fly)

Use

P insertion vector

Tags

myc epitope

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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opto-E-cad GFP

Plasmid

#203327

Purpose

Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

Depositor

Seraphine Wegner

Article

Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

Insert

Photoswitchable - E-cadherin with GFP tag (CDH1 Human)

Tags

GFP

Expression

Mammalian

Mutation

Addition of Aslov2 domain at T133 of human E-cadh…

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pNL2119 pARF7 GG0-GG1

Plasmid

#195910

Purpose

Level 0 pARF7 promoter with GG0 and GG1 golden gate linkers

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

pARF7 (NPH4 Mustard Weed)

Use

Synthetic Biology

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-mSmo

Plasmid

#25395

Depositor

Philip Beachy

Article

Chen et al Genes Dev. 2002 Nov 1. 16(21):2743-8.

Insert

mouse Smoothened cDNA (Smo Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only

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pRSF-T20S

Plasmid

#110805

Purpose

expresses Thermoplasma acidophilium 20S proteasome in E. Coli

Depositor

Yifan Cheng

Article

Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

Inserts

PsmB
PsmA

Tags

tev-His

Expression

Bacterial

Mutation

N2D and Q2E

Promoter

T7 and T7 promoter

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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HA-dora[A]_rescue_construct

Plasmid

#190640

Purpose

Puromycin-selectable expression of HA-tagged truncated Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Tags

3xHA

Expression

Insect

Mutation

Truncated to contain amino acids 1-945

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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