We narrowed to 13,947 results for: cas9

1. pSH231-EFS-Cas9-BlastR

   Plasmid

   #115146

   Purpose

   Safe harbor site 231 knock-in vector with Cas9-BlastR expression cassette

   Depositor

   Michael Phelps, Raymond Monnat

   Article

   Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

   Insert

   Cas9-T2A-BlastR
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX429 SpCas9 N863A nickase

   Plasmid

   #68706

   Purpose

   SpCas9 N863A nickase

   Depositor

   Feng Zhang

   Article

   Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

   Insert

   SpCas9 N863A nicking mutant
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-SpCas9_sgRNA-site1 (RTW443)

   Plasmid

   #160136

   Purpose

   T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #1

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   SpCas9 sgRNA with spacer #1 (spacer=GGGCACGGGCAGCTTGCCGG)
   

   Use

   In vitro transcription of sgrna from t7 promoter

   Promoter

   T7

   Available Since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-Flag-wtSpCas9-H840A

   Plasmid

   #80453

   Purpose

   SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   SpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. 3xFLAG-dCas9/pTEF1p-CYC1t

   Plasmid

   #62190

   Purpose

   Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Yeast

   Mutation

   human codon-optimized, D10A, H840A

   Promoter

   TEF1 promoter

   Available Since

   Feb. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. MiniCoopR U6:gRNA cdkn2a, mitfa:Cas9

   Plasmid

   #118842

   Purpose

   Targets cdkn2a and expresses zebrafish mitfa specifically in zebrafish melanocytes

   Depositor

   Leonard Zon

   Article

   Ablain et al Science. 2018 Nov 1. pii: science.aau650

   Insert

   cdkn2a gRNA (LOC100329528 Zebrafish)
   

   Use

   CRISPR; Tol2

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

   Plasmid

   #129530

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIC3Nme
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-GfABC1D-SaCas9-WPRE3-pA

   Plasmid

   #203540

   Purpose

   SaCas9 expression in astrocytes

   Depositor

   Ben Deverman

   Article

   Huang et al PLoS Biol. 2023 Jul 19;21(7):e3002112. d

   Insert

   SaCas9
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. p2T-CAG-KKHSaCas9-BlastR

   Plasmid

   #107189

   Purpose

   Confers constitutive expression of KKH SaCas9.

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Insert

   KKH SaCas9 (NEWENTRY )
   

   Expression

   Bacterial

   Mutation

   E782K/N968K/R1015H

   Promoter

   Chicken ß-Actin

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD_A-CmYLCV-dCas9-24XGP41

    Plasmid

    #202009

    Purpose

    Moclo level 1 - Module A, Promoter: CmYLCV, Gene: dCas9-24XGP41, Terminator: HSP

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Insert

    dCas9-24XGP41
    

    Use

    Synthetic Biology; Moclo level 1 vector

    Tags

    GS linkers

    Promoter

    CmYLCV

    Available Since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-VPR-dSpCas9[StaPL(TI)]

    Plasmid

    #111508

    Purpose

    Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].

    Depositor

    Michael Lin

    Article

    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi

    Insert

    VPR-dSpCas9[StaPL(TI)]
    

    Expression

    Mammalian

    Mutation

    The HCV NS3 protease carries F43L, Q80K, and S122…

    Promoter

    PGK

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-SMVP-Cas9N-U6-gRNA P2

    Plasmid

    #80944

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 binding

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-FLEX-SaCas9-U6-sgGrin2a

    Plasmid

    #124850

    Purpose

    Mutagenesis of Grin2a

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin2a (Grin2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p-dCas9-LSD1-K661A-Hygro

    Plasmid

    #104414

    Purpose

    transient expression of dCas9-dLSD1 fusion protein

    Depositor

    Stephan Beck

    Insert

    dLSD1 (KDM1A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    IRATp970A0364D (Source Bioscience) has a P405H ch…

    Promoter

    CMV promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CMV-SauriCas9-Puro

    Plasmid

    #135965

    Purpose

    Expresses SauriCas9 and puromycin resistance genes, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pnos-Fok1:dCas9-nos

    Plasmid

    #62210

    Purpose

    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Insert

    Fok1-dCas9
    

    Expression

    Insect

    Promoter

    nanos

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9-CRY2Clust-FUSN-mut

    Plasmid

    #200970

    Purpose

    induction of phase-separated condensate mediated by FUSN-IDR mutant

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    FUSNmut
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Mutation

    15 Tyr-to-Ser substitutions

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PET-B-eSpCas9-NLS-6xHis

    Plasmid

    #207383

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    K848A, K1003A, R1060A, amino acids 1005-1013 repl…

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a8

    Plasmid

    #124861

    Purpose

    Mutagenesis of Slc17a8

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a8 (Slc17a8 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid

    #98975

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 4 and Cas9(D10A)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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