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174,565 results

Showing: 9241 - 9250 of 174565 results

  2. ATP1A1_plasmid_donor_RD

    Plasmid
    #86551
    Purpose
    Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n
    Insert
    ATP1A1 (ATP1A1 Human)
    Use
    CRISPR and TALEN; Co-selection via hdr using ouab…
    Expression
    Mammalian
    Mutation
    Q118R, N129D
    Available Since
    March 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    86551-plasmid-map-sequence-id-171421

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  3. bZiPro

    Plasmid
    #86846
    Purpose
    express NS2B-NS3 protease in E.coli
    Depositor
    Dahai Luo
    Article
    Zhang et al Science. 2016 Dec 8. pii: aai9309.
    Inserts
    NS3 1-177
    NS2B45-96
    Tags
    a hexahistidine tag and a thrombin cleavage site
    Expression
    Bacterial
    Mutation
    NS2B cofactor region (residues 45–96) and NS3 pro…
    Promoter
    T7
    Available Since
    March 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    86846-plasmid-map-sequence-id-172845

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  7. pTRIPZ (M)-HT-Cbx2

    Plasmid
    #82510
    Purpose
    Expresses Halotag-Cbx2 fusion proteins in mammalian cells
    Depositor
    Xiaojun Ren
    Insert
    Chromobox Homolog 2 (CBX2 Human)
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    aggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaacc…
    Available Since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    82510-plasmid-map-sequence-id-156621

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Showing: 9241 - 9250 of 174565 results