We narrowed to 13,583 results for: TIM

1. pcDNA5/FRT/TO_Lifeact_STf

   Plasmid

   #182011

   Purpose

   CMV overexpression of SNAP-tag fast in mammalian cell lines associated to actin

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   Lifeact_STf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. ptagBFP-LacI

   Plasmid

   #103839

   Purpose

   Flourescent marker for lacO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   tagBFP-LacI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   LacI: C19T (silent, L7L), T264C (silent, A88A), C…

   Promoter

   SV2

   Available since

   Dec. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5/FRT/TO_TOMM20-STf

   Plasmid

   #182002

   Purpose

   CMV overexpression of SNAP-tag fast at the mitochondria surface of mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

   Insert

   TOMM20-STf
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Fast mutation on SNAP-tag E30R

   Promoter

   CMV

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAC1750

   Plasmid

   #127984

   Purpose

   AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)

   Depositor

   Uffe Mortensen

   Article

   Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

   Insert

   Cpf1
   

   Use

   CRISPR; Fungal expression

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   codon optimized for Aspergillus nidulans

   Promoter

   Aspergillus nidulans tef1 promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. EKAR2G_design1_mTFP_175_Venus_173

   Plasmid

   #39830

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   Erk activity reporter
   

   Use

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pZR064_CROPseq-sgCD4-CRISPRi

   Plasmid

   #180270

   Purpose

   Lentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRi

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   CD4 sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+/N-SNAPf

   Plasmid

   #184415

   Purpose

   vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

   Depositor

   Ann Miller

   Article

   Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

   Type

   Empty backbone

   Use

   For mrna synthesis

   Tags

   SNAPf-tag

   Expression

   Mutation

   Codon optimized for expression in Xenopus laevis …

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2+/C-SNAPf

   Plasmid

   #184416

   Purpose

   vector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

   Depositor

   Ann Miller

   Article

   Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

   Type

   Empty backbone

   Use

   For mrna synthesis

   Tags

   SNAPf-tag

   Expression

   Mutation

   Codon optimized for expression in Xenopus laevis …

   Promoter

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. KA1158_pPBCAG-MerCreMer-IZ

   Plasmid

   #124184

   Purpose

   piggyBac-based expression of the tamoxifen-inducible Cre

   Depositor

   Hans Schöler

   Article

   Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

   Insert

   MerCreMer-IRES-Zeo
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKD317.8

    Plasmid

    #181951

    Purpose

    IPTG-inducible expression of NarX

    Depositor

    Jeffrey Tabor

    Article

    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

    Insert

    NarX; lacI (narX, lacI NarX - E. coli; lacI - E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    NarX-Ptac; lacI-PlacIq

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-T7-SpCas9-NG-P2A-mScarlet (MNW005)

    Plasmid

    #174140

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-NG(L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-NG with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    SpCas9-NG=L1111R/D1135V/G1218R/E1219F/A1322R/R133…

    Promoter

    CMV and T7

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTrichoGate-4

    Plasmid

    #160704

    Purpose

    Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    optimised synthetic mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUCBB-ntH6-eGFP

    Plasmid

    #32557

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    6 His tag with Thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEB1-mCerulean ME

    Plasmid

    #103971

    Purpose

    Plasmid encoding mCerulean ME

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mCerulean ME
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, S65T, …

    Promoter

    proC

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. FKBP-CD28TMD-NTEVp(WT)

    Plasmid

    #137826

    Purpose

    MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FKBP-CD28TMD-NTEVp(WT)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPDS1-mEGFP-IRES-puro2b

    Plasmid

    #26464

    Depositor

    Daniel Gerlich

    Article

    Held et al Nat Methods. 2010 Sep . 7(9):747-54.

    Insert

    pPDS1 (PTTG1 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTriExEKAR2G1

    Plasmid

    #40174

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pB80-iLID-mCherry-RAB5

    Plasmid

    #174624

    Purpose

    Optogenetic coupling to early endosomes (RAB5) via iLID

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    iLID-mCherry-RAB5 (RAB5A Human, Synthetic)
    

    Use

    Tags

    iLID-mCherry

    Expression

    Mammalian

    Mutation

    mCherry: Met1Del; RAB5: Met1Del

    Promoter

    Chicken beta-actin

    Available since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-nlsGal80(opt)

    Plasmid

    #51618

    Purpose

    vertebrate expression of codon-optimized Gal80 with N-terminal NLS and no stop

    Depositor

    Joshua Bonkowsky

    Article

    Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

    Insert

    Gal80(opt) (GAL80 Budding Yeast)
    

    Use

    Vertebrate expression

    Tags

    NLS

    Expression

    Mutation

    codon optimized

    Promoter

    Available since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KA1875_pGL4-UBCmin-luc2/Sox2_SRR2

    Plasmid

    #124198

    Purpose

    Luciferase reporter driven by the Sox2 enhancer

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    Sox2 enhancer
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    UBCmin

    Available since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCytERM-mTagBFP2

    Plasmid

    #184940

    Purpose

    ER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    CytERM-mTagBFP2
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pETM6-At4CL-CmCHS-CmCHI

    Plasmid

    #73395

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    CmCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-b2AR-hLOV1-TEVcs-FLAG-GAL4-V5

    Plasmid

    #104842

    Purpose

    b2AR-SPARK TF component

    Depositor

    Alice Ting

    Article

    Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

    Insert

    hLOV1-TEVcs-FLAG-GAL4-V5
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. EKAR2G_design1_mTFP_105_Venus_157

    Plasmid

    #39819

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

25. pILGFPQ4

    Plasmid

    #83559

    Purpose

    Used to evaluate the expression output of GAL7 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    GAL7 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTRF.1-mCMV-udsVenus

    Plasmid

    #58518

    Purpose

    Empty lentiviral expression vector for ultradestabilized Venus that indicates the activity of a transcriptional element of interest

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    None
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUC19-U6-crRNA4n96

    Plasmid

    #123640

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    crRNA scaffold 4n96
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EKAR2G_design1_mTFP_159_Venus_173

    Plasmid

    #39825

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EKAR2G_design1_mTFP_175_Venus_195

    Plasmid

    #39831

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

30. XE117 CAMKII-CS2+

    Plasmid

    #16737

    Depositor

    Randall Moon

    Article

    Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

    Insert

    CAMKII (Camk2a Rat)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only