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We narrowed to 6,530 results for: nls

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Showing: 3681 - 3720 of 6530 results

  1. pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis

    Plasmid
    #246982
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    φC31(PhiC31)
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    246982-plasmid-map-sequence-id-489535

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  2. HaloTag-LacI-KRAB

    Plasmid
    #232638
    Purpose
    lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with Krüppel-associated box (KRAB) repressor
    Depositor
    Karsten Rippe
    Insert
    HaloTag-LacI-KRAB
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232638-plasmid-map-sequence-id-462856

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  6. pKG194

    Plasmid
    #69757
    Purpose
    Mammalian Expression of CENP-T aa 1-375
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-375 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and eGFP
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Available Since
    Nov. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. P2814

    Plasmid
    #248829
    Purpose
    All repressors for the WTC848 system.
    Depositor
    Joerg Stelling
    Insert
    LacI(6m), LacI(5m)
    Tags
    SV40NLS on LacI(6m)
    Expression
    Yeast
    Mutation
    LacI(6m): T167A, W220F, M254L, V119I, Q60G, M242L…
    Promoter
    pRnr2 (LacI(6m), P4Lacn.2 (LacI(5m))
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248829-plasmid-map-sequence-id-497158

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  12. P2880

    Plasmid
    #248830
    Purpose
    All repressors for the WTC848 system.
    Depositor
    Joerg Stelling
    Insert
    LacI(6m), LacI(5m)
    Tags
    SV40NLS on LacI(6m)
    Expression
    Yeast
    Mutation
    LacI(6m): T167A, W220F, M254L, V119I, Q60G, M242L…
    Promoter
    pRnr2 (LacI(6m), P4Lacn.2 (LacI(5m))
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248830-plasmid-map-sequence-id-497160

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  13. P2881

    Plasmid
    #248831
    Purpose
    All repressors for the WTC848 system.
    Depositor
    Joerg Stelling
    Insert
    LacI(6m), LacI(5m)
    Tags
    SV40NLS on LacI(6m)
    Expression
    Yeast
    Mutation
    LacI(6m): T167A, W220F, M254L, V119I, Q60G, M242L…
    Promoter
    pRnr2 (LacI(6m), P4Lacn.2 (LacI(5m))
    Available Since
    Jan. 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248831-plasmid-map-sequence-id-497164

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  14. (915) SB EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195623
    Purpose
    Sleeping Beauty EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    EF1a (Mlu1/EcoR1)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195623-plasmid-map-sequence-id-385117

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  15. (942) pAAV EF1a dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195624
    Purpose
    pAAV EF1a plasmid for constitutive expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Use
    AAV
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    EF1a (Mlu1/EcoR1)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195624-plasmid-map-sequence-id-385116

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  16. pAAV-MP-84-spCas9-U6-gRNA-tlock

    Plasmid
    #206936
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    MP-84
    Available Since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    206936-plasmid-map-sequence-id-407723

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  23. mTagRFP-T2-PCNA-19

    Plasmid
    #58043
    Purpose
    Localization: Nucleus, Excitation: 555, Emission: 584
    Depositor
    Michael Davidson
    Insert
    PCNA (PCNA Human)
    Tags
    mTagRFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 4, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    58043-plasmid-map-sequence-id-175700

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  24. pdCas9-tdTomato-VPR

    Plasmid
    #232632
    Purpose
    VPR activation domain coupled to catalytically inactive dCas9 for localization; contains tdTomato for labeling and as a spacer
    Depositor
    Karsten Rippe
    Insert
    dCas9-tdTomato-VPR
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    232632-plasmid-map-sequence-id-462149

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  25. pcDNA3.1-CRISPR-Puro

    Plasmid
    #208646
    Purpose
    pcDNA3.1 based empty vector for transient transfection of sgRNA-cas9. U6 promoter ~ puro Res cloned from lentiCRISPRv2 (Addgene#52961), lenti virus sequences not included.
    Depositor
    Quanfu Ma
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Clawed frog nucleoplasmin NLS, Flag, Puromycin re…
    Expression
    Mammalian
    Promoter
    CMV and U6 in tandem
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208646-plasmid-map-sequence-id-418362

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  29. pET28a(+)v2_TwinStrep-SUMO-FLP-8xHis

    Plasmid
    #246980
    Purpose
    Expresses E. coli codon-optimized TwinStrep-SUMO-tagged FLP recombinase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.
    Depositor
    Amro Hamdoun
    Insert
    FLP Recombinase
    Use
    Synthetic Biology
    Tags
    8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrep
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    246980-plasmid-map-sequence-id-491526

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  30. (829) SB Tet-ON dCasphi-[myc-ZIM3-HA] U6-gStop

    Plasmid
    #195622
    Purpose
    Sleeping Beauty Tet-ON plasmid for induced expression of dCasphi-ZIM3 CRISPRi construct
    Depositor
    Tonia Rex
    Insert
    dCasphi
    Tags
    C-Myc (NLS), HA tag, and ZIM3
    Expression
    Mammalian
    Mutation
    P749A and P753A
    Promoter
    Tight TRE promoter; Tet-ON (doxycycline)
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    195622-plasmid-map-sequence-id-385115

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  31. GLuc VLP

    Plasmid
    #182213
    Purpose
    YIp for expressing MPyV deltaVP1 (GAL1 promoter) and Gaussia luciferase tagged with VP2C (GAL10 promoter).
    Depositor
    Claudia Vickers
    Inserts
    VP2C-GLuc
    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available Since
    March 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182213-plasmid-map-sequence-id-357719

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Showing: 3681 - 3720 of 6530 results