We narrowed to 24,628 results for: Nes

1. CT-Nano-lantern(ATP1)/pRSETB

   Plasmid

   #51990

   Purpose

   bacterial expression of a Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_ATP1
   

   Use

   Tags

   6xHis and chloroplast transit peptid

   Expression

   Bacterial

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pMaCTag-P04

   Plasmid

   #120015

   Purpose

   Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mTFP1

   Depositor

   Michael Knop

   Article

   Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

   Insert

   mTFP1
   

   Use

   Pcr template

   Tags

   mTFP1

   Expression

   Mutation

   Promoter

   None

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMA4.1 (mouse integrin alpha 4 probe)

   Plasmid

   #14098

   Depositor

   Richard Hynes

   Article

   Yang et al Development. 1995 Feb . 121(2):549-60.

   Insert

   Mouse Alpha 4 Integrin (Itga4 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NGN3-T2A-NLS-tdTomato-ires-puro-BGHpA-PGK-puro

   Plasmid

   #89994

   Purpose

   Donor template for generation of NGN3-ntdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   NGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   none

   Available Since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDGB3_omega1

   Plasmid

   #68238

   Purpose

   GoldenBraid level 1 Omega plant expression vector

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   empty vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDGB3_alpha2

   Plasmid

   #68229

   Purpose

   GoldenBraid level 1 Alpha plant expression vector.

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   empty vector
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Oct. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSLIK TT 3xFLAG LacZ neo

   Plasmid

   #83105

   Purpose

   Lentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tag

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   LacZ
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

8. Khc73-mCherry

   Plasmid

   #31752

   Depositor

   Ron Vale

   Article

   Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

   Insert

   Khc73 (Khc-73 Fly)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Metallothionein

   Available Since

   Sept. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

9. Nano-lantern(Ca2+)_11/pRSETB

   Plasmid

   #51981

   Purpose

   bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=11nM

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern_CaM-4GS
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S257G in Rluc8

   Promoter

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pFAST-BAC-Cdc20wt (2193)

    Plasmid

    #39850

    Depositor

    Jonathon Pines

    Article

    Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

    Insert

    Cdc20 (CDC20 Human)
    

    Use

    Baculovirus expression

    Tags

    His

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pJK413

    Plasmid

    #155372

    Purpose

    dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CT-Nano-lantern(ATP1)/pENTR1A

    Plasmid

    #51991

    Purpose

    Gateway entry vector for Nano-lantern-based luminescent ATP indicator, targeted to chloroplasts

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_ATP1
    

    Use

    Entry vector

    Tags

    chloroplast transit peptid

    Expression

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pMaCTag-Z18

    Plasmid

    #120061

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mKeima8.5

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mKeima8.5
    

    Use

    Pcr template

    Tags

    mKeima8.5

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEIIIA

    Plasmid

    #14059

    Depositor

    Richard Hynes

    Article

    Paul et al J Biol Chem. 1986 Sep 15. 261(26):12258-

    Insert

    Rat Fibronectin EIIIA (Fn1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EcoRI linkers added to TaqI to HinfI fragment

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

15. nPEX-LV

    Plasmid

    #12119

    Depositor

    Inder Verma

    Article

    Pfeifer et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

    Insert

    Matrix Metalloproteinase 2 (Mmp2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    noncatalytic fragment of MMP2 amplified via PCR, …

    Promoter

    Available Since

    April 27, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pLX302-NIPP1-V5 puro

    Plasmid

    #87775

    Purpose

    Expresses V5 tagged human NIPP1

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    NIPP1 (PPP1R8 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Nano-lantern(ATP1)/pRSETB

    Plasmid

    #51988

    Purpose

    bacterial expression of a Nano-lantern-based luminescent ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_ATP1
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Nano-lantern(Ca2+)_63/pcDNA3

    Plasmid

    #51986

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-4GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pEN_TTmiRc2_BirA

    Plasmid

    #136521

    Purpose

    Used as a donor vector to clone into pSLIK

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    BirA
    

    Use

    Entry vector for gateway cloning

    Tags

    Expression

    Mutation

    R118G (highly promiscuous form)

    Promoter

    Available Since

    June 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLIK TT 3xFLAG LacZ hygro

    Plasmid

    #98395

    Purpose

    Lentiviral vector for inducible expression of LacZ with 3xFLAG N-terminal tag

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    LacZ
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJK429

    Plasmid

    #155376

    Purpose

    PphlF_A4NT in dCRv1, 1x cascade + [dCas9 + PA4-mVenus]

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas9 (bacteria)
    PA4-mVenus
    PphlF_A4NT in dCas9 sgRNA oscillator v1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pEN_TT miRc2 3xFLAG

    Plasmid

    #83274

    Purpose

    Empty entry vector for cloning inducible mammalian expression and an in-frame N-terminal 3xFLAG

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Type

    Empty backbone

    Use

    Entry vector for gateway cloning

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    TRE tight

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDEST-Hemmar-FLP(D)

    Plasmid

    #119895

    Purpose

    FLP(D) with a hsp70 promoter

    Depositor

    Rita Sousa-Nunes

    Article

    Chai et al Elife. 2018 Nov 27;7. pii: 38393. doi: 1

    Insert

    FLP(D)
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    mutated form of FLP, which at position 5 contains…

    Promoter

    Available Since

    Feb. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. SEPLuc

    Plasmid

    #141102

    Purpose

    bioluminescent reporter consisting of a membrane-bound SEP and Nanoluc fusion; for monitoring extracellular tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    SEP-Nanoluc
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1α

    Available Since

    May 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcFN-C (chick fibronectin)

    Plasmid

    #14063

    Depositor

    Richard Hynes

    Article

    Norton et al Mol Cell Biol. 1987 Dec . 7(12):4297-307

    Insert

    Chicken Fibronectin (FN1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    DraI - EcoRI fragment inserted in HincII-EcoRI si…

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pENTR221-WNT3

    Plasmid

    #79500

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human WNT3 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    WNT3 (WNT3 Human)
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pMaCTag-Z04

    Plasmid

    #120047

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mTFP1

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mTFP1
    

    Use

    Pcr template

    Tags

    mTFP1

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMaCTag-P30

    Plasmid

    #120041

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with VSV-G

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    VSV-G
    

    Use

    Pcr template

    Tags

    VSV-G

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Nano-lantern(ATP0)/pRSETB

    Plasmid

    #51989

    Purpose

    bacterial expression of a Nano-lantern-based luminescent ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_ATP0
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

30. Nano-lantern(ATP1)/pENTR1A

    Plasmid

    #51993

    Purpose

    Gateway entry vector for Nano-lantern-based luminescent ATP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_ATP1
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

31. pMaCTag-25

    Plasmid

    #120004

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with YFP-LID

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    YFP-LID
    

    Use

    Pcr template

    Tags

    YFP-LID

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMaCTag-19

    Plasmid

    #124787

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry-mNeonGreen

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mCherry-mNeonGreen
    

    Use

    Pcr template

    Tags

    mCherry-mNeonGreen

    Expression

    Mutation

    Promoter

    None

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pDGB3_alpha1R

    Plasmid

    #68230

    Purpose

    GoldenBraid level 1 Alpha plant expression vector.

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    empty vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pPromE8 (GB0914)

    Plasmid

    #68175

    Purpose

    Provides the S. Lycopersicum fruit promoter E8 as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    SlE8 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Nano-lantern(Ca2+)_117/pRSETB

    Plasmid

    #51979

    Purpose

    bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=117nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM-2GS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

36. Nano-lantern(Ca2+)_150/pRSETB

    Plasmid

    #51978

    Purpose

    bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=150nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM-2G
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S257G in Rluc8

    Promoter

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

37. pT35S (GB0036)

    Plasmid

    #68187

    Purpose

    Provides the CaMV 35S terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    CaMV 35S terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMaCTag-P03

    Plasmid

    #120014

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    moxCerulean3
    

    Use

    Pcr template

    Tags

    moxCerulean3

    Expression

    Mutation

    Promoter

    None

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLSLZ

    Plasmid

    #51495

    Purpose

    pLSL with Zif268:FokI coding sequence followed by approximately 3kb 'filler' sequence, Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    Zif268:FokI
    3kb filler sequence
    

    Use

    Plant t-dna plasmid

    Tags

    SV40 NLS

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available Since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJT3

    Plasmid

    #98304

    Purpose

    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of CUP1 promoter

    Depositor

    Claudia Vickers

    Article

    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

    Insert

    TRPL3AcNES1>TRPL41B
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge