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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,624 results for: RAN-1

Showing: 4651 - 4700 of 18624 results

  1. pIBA3-SpyCatcherEQ-sfGFP

    Plasmid
    #107421
    Purpose
    For production of inactive SpyCatcherEQ protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    SpyCatcher
    Use
    Tags
    His tag, StrepII, and superfolder GFP
    Expression
    Bacterial
    Mutation
    mutation of glutamate 77 to glutamine
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107421-plasmid-map-sequence-id-207622

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  2. pIBA2-SpyTag-MBP

    Plasmid
    #107422
    Purpose
    Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    Maltose-binding protein (MalE)
    Use
    Tags
    OmpA signal peptide, SpyTag, and StrepII
    Expression
    Bacterial
    Mutation
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107422-plasmid-map-sequence-id-212955

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  3. pTS2381-Tier1-PhCMV-Igk_TM

    Plasmid
    #169487
    Purpose
    Tier-1 vector encoding PhCMV-driven transmembrane domain from Junctophilin-1 (TM) for membrane localization of fusion proteins attached to a secretion signal Igk (PhCMV-IgkSS-TM-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven membrane localization
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PhCMV
    Available Since
    June 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    169487-plasmid-map-sequence-id-364214

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  15. pGR596

    Plasmid
    #213787
    Purpose
    Integrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    See manuscript
    Promoter
    Available Since
    March 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    213787-plasmid-map-sequence-id-424625

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  19. pSN-A112C-GpA

    Plasmid
    #191238
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    Use
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine
    Promoter
    T7 promoter
    Available Since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191238-plasmid-map-sequence-id-380469

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  31. CC1

    Plasmid
    #63713
    Purpose
    Wild-type voltage sensing domain from Ciona voltage sensing phosphatase fused to super ecliptic A227D with restriction site flanking each transmembrane domain
    Depositor
    Bradley Baker
    Article
    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi
    Insert
    CC1
    Use
    Tags
    Super ecliptic pHlorin A227D
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    July 27, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 4651 - 4700 of 18624 results