We narrowed to 7,572 results for: Lif

1. pCAG-WIPI2d10-364delta263-295-TEV-STREP

   Plasmid

   #171830

   Purpose

   Mammalian expression of human WIPI2d 10-364 delta263-295 with C-terminal Strep

   Depositor

   James Hurley

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2d (WIPI2 Human)
   

   Use

   Tags

   Strep

   Expression

   Mammalian

   Mutation

   10-364delta263-295

   Promoter

   Available since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUE1-TSP-ORF68 K395A/K396A

   Plasmid

   #162656

   Purpose

   Expresses ORF68 K395A/K396A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Amino acids K395 and K396 mutated to alanine

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJP72

   Plasmid

   #176479

   Purpose

   Expresses mScarlet under control of the Capsaspora EF1 promoter and GenR under control of the Capsaspora actin promoter

   Depositor

   Duojia Pan

   Article

   Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

   Inserts

   actin > GenR
   EF1 > mScarlet
   

   Use

   Capsaspora owczarzaki expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Halo-WIPI2B L69E

   Plasmid

   #175026

   Purpose

   Expresses WIPI2B (L69E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

   Depositor

   Andrea Stavoe

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2B (L69E) (WIPI2 Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Leucine 69 to Glutamate (CTA to GAA)

   Promoter

   CMV

   Available since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJH3409

   Plasmid

   #179574

   Purpose

   Pceh-12::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans VB-MN -specific expression of miniSOG-SL2 RFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Use

   Tags

   wCherry

   Expression

   Worm

   Mutation

   Promoter

   Pceh-12

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. MS2-adRNA (3'UAG)

   Plasmid

   #170128

   Purpose

   Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   MS2-ADAR2(3'UAG)-MS2
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and mouse U6

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet

   Plasmid

   #83286

   Purpose

   untargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pair

   Depositor

   Daniel Needleman

   Article

   Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

   Insert

   mTurquoise2-FHA2-AURKB substrate-YPet
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXW109ABS84(RS)

   Plasmid

   #123155

   Purpose

   Amplified arsenic sensor with double ArsR binding sites. pSB3K3 carrying J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-30arsR-t-ParsR-ABS84-30hrpR-30hrpS-t-PhrpLE-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J109, ParsR-ABS84, PhrpLE

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-SAC1-HLx8

   Plasmid

   #108139

   Purpose

   SAC1 with extended linker

   Depositor

   Gerry Hammond

   Article

   Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

   Insert

   mCherry:SACM1L(1-520):[EAAAR]8:SACM1L(521-587) (SACM1L Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SACM1L(1-520):[EAAAR]8:SACM1L(521-587)

   Promoter

   CMV

   Available since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEM01

    Plasmid

    #135414

    Purpose

    Expression of Hygromycin resistance from CMV promoter

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    CMVpr hph ADH1t
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    CMVpr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. JB96

    Plasmid

    #128923

    Purpose

    mammalian expression, proximity labelling, mitochondrial relocalisation

    Depositor

    Sean Munro

    Article

    Gillingham et al Elife. 2019 Jul 11;8. pii: 45916. doi: 1

    Insert

    nRasSN-BirA*-HA-MAO (NRAS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. SMT205

    Plasmid

    #134817

    Purpose

    SMT201 with mutated RBS for DHFR, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Pub-mCD8:GFP-T2A-DsRed:NLS-SV40

    Plasmid

    #130665

    Purpose

    Plasmid to test the efficiency of T2A multiplexing in Aedes aegypti

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    AaegPUb-mCD8-GFP-T2A-dsRed-NLS-SV40
    

    Use

    Ae. aegypti expression

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Aedes aegypti poly-ubiquitin

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-ArchT-GFP

    Plasmid

    #86536

    Purpose

    Retrovirus expressing optogenetic silencer ArchT-GFP

    Depositor

    Xue Han

    Article

    Zhuo et al Elife. 2016 Dec 3;5. pii: e22429. doi: 1

    Insert

    ArchT
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin I (human)

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac Dual PNG-FLAG PLU-HIS

    Plasmid

    #113173

    Purpose

    Baculovirus expression of PNG and PLU

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    PNG, PLU
    

    Use

    Baculovirus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJH104

    Plasmid

    #48262

    Purpose

    Template plasmid for PCR-based transplant of Saccharomyces cerevisiae TEF1 promoter in yeast.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10

    Inserts

    TEF1p 5' fragment
    URA3 5' fragment
    TEF1p 3' fragment
    

    Use

    Routine cloning vector

    Tags

    Expression

    Mutation

    From SK1 background. -343 to -1 relative to TEF1 …

    Promoter

    TEF1p

    Available since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET151-Vta1(280-330) S278C

    Plasmid

    #87739

    Purpose

    Bacterial expression of a VSL domain variant of S. cerevisiae Vta1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-277, mutated Ser 278 to Cys

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. CSII-IDR2-chimpTRIM5-FOS

    Plasmid

    #79065

    Purpose

    Express chimpanzee TRIM5alpha in mammalan cells

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    TRIM5alpha(chimpanzee)-FOS
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET151-Vps4(81-437)

    Plasmid

    #87734

    Purpose

    Bacterial expression of N-terminally truncated S. cerevisiae Vps4

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-80

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. FRB-GFP-GCN4-ppKin14

    Plasmid

    #240427

    Purpose

    -end oriented kinesin for rapalog induced pulling experiments.

    Depositor

    Anna Akhmanova

    Article

    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

    Insert

    ppKin14Vib
    

    Use

    Tags

    FRB, GCN4, and GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-850

    Promoter

    Available since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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