We narrowed to 2,981 results for: erf

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pCellFree_G03 RAP2A

Plasmid

#67058

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

RAP2A (RAP2A Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 BTF3

Plasmid

#67059

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BTF3 (BTF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 MAPK13

Plasmid

#67077

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

MAPK13 (MAPK13 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-hfYFP-TEV-SAV

Plasmid

#191197

Purpose

Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & Streptavidin fusion

Tags

6xHis and Streptavidin core domain, amino acids 1…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC25A46[I349D]-His (SB256)

Plasmid

#227605

Purpose

Inducible expression of His-tagged SLC25A46 with I349D mutation in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

10xHis

Expression

Yeast

Mutation

I349D

Promoter

AOX1

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-MAVS(IRSp53 pLxIS)

Plasmid

#221315

Purpose

Expression of MAVS chimera with IRSp53 pLxIS domain in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS chimera with IRSp53 pLxIS domain (Mavs Mouse)

Use

Retroviral

Tags

FLAG

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-TASL-NEMO

Plasmid

#221267

Purpose

Expression of TASL-NEMO IKK binding site in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

fusion of TASL with NEMO IKK binding site (Tasl, Ikbkg Mouse)

Use

Retroviral

Tags

3xFLAG

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-iStat3-BFP

Plasmid

#216870

Purpose

Lenti-iStat3-BFP plasmid contains the TRE (doxycycline inducible) promoter followed by the Stat3 mouse cDNA, a T2A and a Blue Fluorescent Protein (BFP) sequence.

Depositor

Bernhard Payer

Article

Barrero et al Sci Adv. 2024 Aug 9;10(32):eadj8862. doi

Insert

Stat3 (Stat3 Mouse)

Use

Lentiviral

Tags

T2A-TagBFP

Expression

Mammalian

Promoter

Tetracycline-Response-Element (TRE)

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCDF-B-pC

Plasmid

#213495

Purpose

Encodes Node B of the three-node Turing circuit

Depositor

Mark Isalan

Inserts

cinI (cinI )
lacI (lacI )
lambda cI (lambdap50 )
sfGFP

Use

Synthetic Biology

Tags

ASV tag and LVA tag

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ265E6

Plasmid

#213469

Purpose

Gateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editing

Depositor

Yiping Qi

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Insert

TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI

Use

CRISPR; Gateway compatible tada-cda-32aa linker-z…

Tags

No

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD-BpBBS

Plasmid

#199467

Purpose

Arabinose-induced expression of His-tagged biliverdin-binding serpin of Boana punctata treefrog (BpBBS) in E. coli.

Depositor

Vladislav Verkhusha

Article

Manoilov et al J Mol Biol. 2022 Jan 30;434(2):167359. d

Insert

BpBBS

Tags

6xHis

Expression

Bacterial

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-GFP-RELA K310R

Plasmid

#196630

Purpose

Express GFP-RELA K310R

Depositor

Nicolas Manel

Article

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

GFP-RELA K310R (RELA Human)

Use

Lentiviral

Tags

GFP

Mutation

K310R

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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EC0_2_VP64

Plasmid

#163714

Purpose

Plasmid encoding VP64 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

Depositor

Yvonne Nygård

Article

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Insert

VP64 + SV40 NLS

Use

Synthetic Biology; Moclo vector level 0 (type 4a)

Available Since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAPS127A-deltaCC

Plasmid

#166459

Purpose

Expresses fusion of mEGFP and YAPS127A-deltaCC (delta298-359)

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A-deltaCC (YAP1 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1 YAPS127A-deltasTAD

Plasmid

#166461

Purpose

Expresses fusion of mEGFP and YAPdeltasTAD (delta450-504)

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A-deltasTAD (YAP1 Human)

Expression

Mammalian

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S(-10AC12)_Psyn-sgRNArodA

Plasmid

#149661

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi rodA

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNArodA

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30(-10AC12), Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pKM296

Plasmid

#134180

Purpose

Mammalian expression of CENP-C and KNL2 CDK SA

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

mcherry-CENP-C 510-end-KNL2 CDK SA (CENPC Human)

Tags

mCherry

Expression

Mammalian

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX6P1‐Afu rnhB

Plasmid

#108694

Purpose

For expression in E coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII

Depositor

Andrew Jackson, Martin Reijns

Article

Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

Insert

rnhB (AF_RS03165 Archaeoglobus fulgidus)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-ArchT-GFP

Plasmid

#86536

Purpose

Retrovirus expressing optogenetic silencer ArchT-GFP

Depositor

Xue Han

Article

Zhuo et al Elife. 2016 Dec 3;5. pii: e22429. doi: 1

Insert

ArchT

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

Synapsin I (human)

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 PRKCA

Plasmid

#67115

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PRKCA Protein kinase C alpha type (PRKCA Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only