We narrowed to 6,466 results for: sas

1. pCMV-Tag4A-MITF-M (D380N)

   Plasmid

   #31248

   Depositor

   Yardena Samuels

   Article

   Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

   Insert

   MITF-M (MITF Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Aspartic acid 380 changed to Asparagine

   Promoter

   Available since

   July 22, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

   Plasmid

   #69219

   Purpose

   Expresses wild type SpCas9 fused to Zif268 in mammalian cells

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   Zif268 (Egr1 Mouse)
   

   Use

   CRISPR

   Tags

   NLS-3XHA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. PLK3 335-646-pEGFP

   Plasmid

   #23267

   Depositor

   Wei Dai

   Article

   Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

   Insert

   PLK3 (PLK3 Human)
   

   Use

   Tags

   pEGFP

   Expression

   Mammalian

   Mutation

   aa 335-646

   Promoter

   Available since

   Dec. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

   Plasmid

   #184381

   Purpose

   This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

   Depositor

   Ori Bar-Nur

   Article

   Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

   Insert

   Cas9, two gRNAs targeting dystrophin
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-foxc1a

   Plasmid

   #132971

   Purpose

   full-length of foxc1a without stop codon in pmE Gateway vector

   Depositor

   Nathan Lawson

   Article

   Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

   Insert

   foxc1a (foxc1a Zebrafish)
   

   Use

   Gateway vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDS170-enNTS1

   Plasmid

   #199152

   Purpose

   JZ73, bacterial expression construct expressing thermostabilised rat neurotensin receptor 1 (enNTS1)

   Depositor

   Daniel Scott

   Article

   Bumbak et al Cell Rep. 2023 Jan 31;42(1):112015. doi:

   Insert

   enNTS1 (Ntsr1 Rat)
   

   Use

   Tags

   10xHis tag, Avi tag, MBP, and muGFP

   Expression

   Bacterial

   Mutation

   V208M, C273S, C391S and C393S (over NTS1-B5)

   Promoter

   Lac/T5

   Available since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEF1a-RoninDHSA-FLAG-IRES-Neo

   Plasmid

   #28021

   Depositor

   Thomas Zwaka

   Article

   Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

   Insert

   Ronin (THAP11 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   DHSA mutation (Y246A) of the previously defined H…

   Promoter

   Available since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS-f1(-)-GAPDH-IRES-dTomato

   Plasmid

   #115919

   Purpose

   donor to insert IRES-dTomato at the GAPDH locus in human cells

   Depositor

   Erik Sontheimer

   Article

   Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

   Insert

   IRES-dTomato
   

   Use

   Donor template

   Tags

   dTomato

   Expression

   Mutation

   Promoter

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEcRNAP5

   Plasmid

   #128939

   Purpose

   pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z

   Depositor

   Seth Darst

   Article

   Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

   Inserts

   RpoAppx234-241-(His)10 (rpoA Escherichia coli)
   RpoB
   RpoC
   RpoZ
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Residues 234-241 are substituted for a PreScissio…

   Promoter

   T7

   Available since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLKO human shRNA 1 c-MAF

    Plasmid

    #29432

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    c-MAF shRNA 1 (MAF Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pTXB1-hMeCP2-R270X

    Plasmid

    #48092

    Purpose

    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-R270X

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mxe intein/chitin binding domain tagged, expresse…

    Promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pLKO human shRNA 2 c-MAF

    Plasmid

    #29433

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

    Insert

    c-MAF shRNA 2 (MAF Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pJH2827

    Plasmid

    #179542

    Purpose

    Pnmr-1::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 RFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pnmr-1

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTXB1-hMeCP2-G273X

    Plasmid

    #48093

    Purpose

    Bacterial expression of Mxe intein/chitin binding domain tagged MeCP2-G273X

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mxe intein/chitin binding domain tagged, expresse…

    Promoter

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pJH3409

    Plasmid

    #179574

    Purpose

    Pceh-12::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans VB-MN -specific expression of miniSOG-SL2 RFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pceh-12

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PLK3 1-334 T219D-pEGFP

    Plasmid

    #23268

    Depositor

    Wei Dai

    Article

    Yang et al Cancer Res. 2008 Jun 1. 68(11):4077-85.

    Insert

    PLK3 (PLK3 Human)
    

    Use

    Tags

    pEGFP

    Expression

    Mammalian

    Mutation

    aa 1-334 T219D

    Promoter

    Available since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

17. His 3C Kinase dead MKK6 1-334

    Plasmid

    #87882

    Purpose

    E. coli expression of MKK6 kinase dead

    Depositor

    Peter Mace

    Article

    Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Tags

    3C protease site and 6xHis

    Expression

    Bacterial

    Mutation

    K82A, kinase dead mutant

    Promoter

    T7

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. FnoCas12a-DR-GC-swap-cr in pBCSK+

    Plasmid

    #126644

    Purpose

    Cloning vector FnoCas12a-DR-GC-swap-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    FnoCas12a Full Length Direct Repeat GC Swap crRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 Promoter

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS-f1(-)-ACTB-iTagRFP-T

    Plasmid

    #115916

    Purpose

    donor to insert iTagRFP-T at the ACTB locus in human cells

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    ACTB-TagRFP-T
    

    Use

    Donor template

    Tags

    TagRFP-T

    Expression

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBABE MDproER

    Plasmid

    #13495

    Depositor

    Stephen Tapscott

    Article

    Rosenberg et al J Cell Biol. 2006 Oct 9. 175(1):77-85.

    Insert

    MyoD1-Estrogen Receptor Fusion Protein (Myod1, ESR1 Human, Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    full length MyoD (containing the point mutation A…

    Promoter

    Available since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only