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pCas9-NT

Plasmid

#235165

Purpose

Expresses the codon harmonized Streptococcus pyogenes cas9 gene and a NT sgRNA

Depositor

Constantinos Patinios

Article

Della Valle et al Trends Biotechnol. 2025 Jun;43(6):1470-1

Insert

SpCas9

Expression

Bacterial

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pCas12a-NT

Plasmid

#235167

Purpose

Expresses the Wild Type Francisella novicida cas12a gene and a NT crRNA

Depositor

Constantinos Patinios

Article

Della Valle et al Trends Biotechnol. 2025 Jun;43(6):1470-1

Insert

FnCas12a

Expression

Bacterial

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Hygro-eGFP

Plasmid

#99375

Purpose

Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A eGFP from EF-1a promoter. 3rd generation lentiviral backbone.

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Inserts

S. pyogenes sgRNA cassette
Hygro-P2A-eGFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a and hU6

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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eGFP-TRF1 pWzl-Hygro

Plasmid

#19834

Depositor

Titia de Lange

Article

Dimitrova et al Nature. 2008 Nov 27;456(7221):524-8. doi

Insert

Telomere-Repeat binding Factor 1 (Terf1 Mouse)

Use

Retroviral

Tags

enhanced GFP

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pGEP163

Plasmid

#137881

Purpose

PiggyBac donor vector for expression of mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

mRFP-T2A-BlasticidinR

Expression

Mammalian

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAG153 FKBP-CFAST11

Plasmid

#130813

Purpose

Expresses FKBP-CFAST11 in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

FKBP-CFAST11

Tags

Myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-noPS

Plasmid

#177943

Purpose

Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

eGFP

Expression

Mammalian

Available Since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6p1-GST-Rnf31

Plasmid

#63134

Purpose

Expression of human RNF31/HOIP codon optimized for E. coli and insect cells

Depositor

Titia Sixma

Article

Smit et al EMBO J. 2012 Oct 3;31(19):3833-44. doi:

Insert

Rnf31 (RNF31 Synthetic, Human)

Tags

GST

Expression

Bacterial

Mutation

Synthetic based on human protein sequence

Promoter

tac

Available Since

March 16, 2015

Availability

Academic Institutions and Nonprofits only
pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

Plasmid

#138315

Purpose

Lenti expression of mTgBFP2

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

mTAGBFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-mDlx-NLS-mRuby2

Plasmid

#99130

Purpose

An AAV genome that expresses nuclear localized mRuby2 from the mouse Dlx5/6 enhancer

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

NLS-mRuby2

Use

AAV

Expression

Mammalian

Promoter

mDLX5/6

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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