159,557 results

1. PB533_2E12LI-sfGFP

   Plasmid

   #167527

   Purpose

   H3K27me3-mintbody expression vector for mammalian cells

   Depositor

   Hiroshi Kimura

   Article

   Tjalsma et al EMBO Rep. 2021 Feb 19:e51989. doi: 10.15

   Insert

   2E12LI-scFv
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

   Plasmid

   #225756

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-HA-USP49

   Plasmid

   #22586

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP49 (USP49 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Dec. 4, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pHR_A08_hrGFP

   Plasmid

   #84615

   Purpose

   HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locus

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   hrGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1a-CRISPRoffv2.1-IRES-BlastR

   Plasmid

   #207180

   Purpose

   Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Insert

   CRISPRoffv2.1
   

   Use

   CRISPR and Lentiviral

   Tags

   HA and TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR FGFR1-CD3ε-FusionRed

   Plasmid

   #188632

   Purpose

   Human FGFR1 labeled with CD3ε ITAMs for monitoring with CD3ε/ZtSH2 pYtag biosensor

   Depositor

   Jared Toettcher

   Article

   Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

   Insert

   FGFR1 (FGFR1 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   CD3ε-FusionRed

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CaMKIIa-hChR2(H134R)-EYFP

   Plasmid

   #26969

   Purpose

   AAV expression of humanized ChR2(H134R) fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Lee et al Nature. 2010 Jun 10. 465(7299):788-92.

   Has Service

   AAV1, AAV2, AAV5, and AAV9

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Histidine 134 is mutated to Arginine

   Promoter

   CaMKIIa

   Available since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-6xGLY-Myc-kanMX6

   Plasmid

   #20769

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S

   Plasmid

   #62458

   Purpose

   expresses his-tagged C151S human Keap1 protein

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Use

   Tags

   His-Halo-Tev

   Expression

   Mammalian

   Mutation

   Changed Cysteine 151 of Keap1 to serine

   Promoter

   CMV

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-Talin head-SpyCatcher003

    Plasmid

    #133566

    Purpose

    Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    EGFP-Talin head-SpyCatcher003
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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