We narrowed to 11,348 results for: Mpl

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pKV016

Plasmid

#64767

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, 3x-HA-GFP to a 3xT7-RFP tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pJAT7

Plasmid

#204292

Purpose

For inserting a phiC31 attP site. HDR integrant is marked with removable 3XP3-DsRed cassette, which is expressed in eyes. DsRed difficult to see in wild type eyes if using LeicaM165 FC microscope.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-DsRed

Use

CRISPR

Promoter

3XP3

Available Since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-3XFLAG-renilla-luciferase-beta-globin-control

Plasmid

#184393

Purpose

Transient transfection plasmid expressing Renilla-luciferase-beta-globin fusion protein

Depositor

Robert Bradley

Article

Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

Insert

Renilla luciferase

Tags

3X FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-LivePAR-Hygro

Plasmid

#176063

Purpose

EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

LivePAR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-SynI-CreOn-FlpOff-mNaChBac-P2A-EGFP

Plasmid

#176280

Purpose

Viral vector for co-expression of NaChBac and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

mNaChBac-P2A-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin I

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMT-Hrp48-ADARcd-E488Q-V5

Plasmid

#139685

Purpose

Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

Depositor

Michael Rosbash

Article

Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

Insert

Hrp48, ADAR (Adar Fly)

Tags

V5

Expression

Bacterial and Insect

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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PB-XL-AncBE4max

Plasmid

#136254

Purpose

Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max

Depositor

Zhaohui Ye

Article

McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

Insert

AncBE4max_GFP

Use

CRISPR; Piggybac dna transposon

Expression

Mammalian

Mutation

The AncBE4max_GFP insert was PCR amplified from a…

Promoter

TRE3G

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS3/myc-His

Plasmid

#127487

Purpose

Expresses myc-tagged NDUFS3 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS3 (NDUFS3 Human)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2/myc-His

Plasmid

#127486

Purpose

Expresses myc-tagged NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Promoter

CMV

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFPC1/GgVcl 1-851 (Vh)

Plasmid

#46268

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

Insert

Vcl 1-851 (VCL Chicken)

Tags

EGFP

Expression

Mammalian

Mutation

1-851 (Vh) head domain

Promoter

CMV

Available Since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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STAGR_SAMScaffold_hU6

Plasmid

#102843

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_hU6promoter

Use

As pcr template

Available Since

Jan. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-R3R2

Plasmid

#105109

Purpose

create your own 2in1 constructs

Depositor

Christopher Grefen

Article

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Use

Other

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-DmrA

Plasmid

#61680

Purpose

GFP-labeled SAH scaffold with DmrA attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-DmrA

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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fSAP24-GST

Plasmid

#11202

Depositor

Robin Reed

Article

Masuda et al Genes Dev. 2005 Jul 1. 19(13):1512-7.

Insert

SAP24 (THOC7 Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only
VSVG-mCherry-RUSH

Plasmid

#203647

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-VSVG

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

VSVG

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159850

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/Venus-rLC3

Plasmid

#58740

Purpose

Expresses Venus-rLC3 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Retroviral

Tags

Venus

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3 βarr1 S412D Flag

Plasmid

#42196

Depositor

Robert Lefkowitz

Article

Luttrell et al Science. 1999 Jan 29. 283(5402):655-61.

Insert

β-arrestin 1 S412D (Arrb1 Rat)

Tags

Flag

Expression

Mammalian

Mutation

S412D

Promoter

CMV

Available Since

Jan. 23, 2013

Availability

Academic Institutions and Nonprofits only
gag-TadCBEd

Plasmid

#225144

Purpose

Packaging plasmid for TadCBEd in v3 PE-eVLP architecture

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-TadCBEd

Expression

Mammalian

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMaCTag-13

Plasmid

#119992

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry

Use

Pcr template

Tags

mCherry

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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