We narrowed to 2,526 results for: T2A
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Plasmid#201556PurposePlasmid with SB ITRs for expressing DBEcR (3xmyc-tagged) under EF1α promoter; blasticidin resistance.DepositorInsertDBEcR
UseUnspecified; Sleeping beauty transposonTagsMycExpressionMammalianPromoterEF1aAvailable SinceJuly 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
eGFP Y93H GC editing reporter
Plasmid#198884PurposeExpress eGFP fluorescence after Y93H correction and mCherry as transfection controlDepositorInsertenhanced green fluorescent protein
UseLentiviralExpressionMammalianMutationY93H, Gly92(GGT-to-GGG)PromoterCMVAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
p-715MRPprom-MRPcucuc
Plasmid#87199PurposeExpression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiencyDepositorAvailable SinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgRNA1_GAL4UAS-Luciferase reporter
Plasmid#64157PurposePhotoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA1 for GAL4UAS-Luciferase reporter
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgRNA2_GAL4UAS-Luciferase reporter
Plasmid#64158PurposePhotoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassetteDepositorInsertsgRNA2 for GAL4UAS-Luciferase reporter
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc
Plasmid#141086Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosisDepositorInsertAntares-T2A-puromycin-EMCV IRES-SEPLuc
ExpressionMammalianMutationDeletion of amino acids 66 to 91 on the Luciferas…PromoterCMVAvailable SinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_mScarlet_Convergent
Plasmid#229790PurposeBxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expressionDepositorInsertTRE-mScarlet; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsNLSExpressionMammalianPromoterTRE and CAGAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCS-3AID-HA-2A-mTurquoiseBSD
Plasmid#225704PurposeEmpty backbone for lentiviral expression of AID degron tagged POI and mTurquoise fused BlasticidinRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsT2A-mTurquoiseExpressionMammalianPromoterpEF1AAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-rTetR(SE)-VP64-2A-mCherryBSD
Plasmid#225695PurposeLentiviral expression of rTetR(SE) fused to VP64 and mCherry fused BlasticidinRDepositorInsertrTetR-VP64
UseLentiviral and Synthetic BiologyTags3xFLAG and T2A-mCherryExpressionMammalianAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiT-GEM(0)
Plasmid#220446PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
ExpressionInsectAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiT-GEM(2)
Plasmid#220448PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 2. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
ExpressionInsectAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiT-GEM(1)
Plasmid#220447PurposeSplit intein version of Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exonsDepositorInsertCfaN-Gal4-CfaC
ExpressionInsectAvailable SinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
vYM245_PB-Blast-pEF1a-H2B-mCh-D3bCI
Plasmid#202596Purposeplasmid for expressing the DNMT3bCI (catalytic inactive)DepositorInsertH2B-mCherry-T2A-rTetR-DNMT3bCI (Dnmt3b Mouse, Synthetic)
UseSynthetic BiologyTagsrTetRExpressionMammalianMutationP656V, C657DAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-Cas9-sgHPRT1
Plasmid#196713PurposeCRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TGDepositorInsertCas9-T2A-BSD-U6-sgHPRT1
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSPromoterEF1a/hU6Available SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
BicC.Ca9.R
Plasmid#168295PurposeExpresses Cas9 in Drosophila late germline cellsDepositorInsertBicC.Cas9-T2A-eGFP-p10
UseCRISPRExpressionInsectAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-pleCopyCatcher
Plasmid#174061PurposeCopyCatcher insertion donor for the pale locusDepositorInsertPale (Pale Synthetic, Fly)
ExpressionBacterialAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1052B (Ubiq-Fluc_Ubiq-Rluc)
Plasmid#132426PurposeExpress Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoterDepositorInsertsUbiq promoter
firefly luciferase
T2A-eGFP
Renilla Luciferase-SV40-P10
reversed ubiq promoter
ExpressionInsectAvailable SinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Antares-SEPLuc IRESv24
Plasmid#141087Purposeprecursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRESDepositorInsertAntares-T2A-puromycin-IRESv24-SEPLuc
ExpressionMammalianPromoterCMVAvailable SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRia-v2
Plasmid#84832PurposeCRISPRi/a V2 library parental plasmidDepositorInsertssgRNA
puro-T2A-BFP
UseCRISPR and LentiviralExpressionMammalianPromotermU6Available SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
murine TCR OTI-2A.pMIG II
Plasmid#52111Purposeretroviral expression of both TCRalpha and TCRbeta using a single vectorDepositorUseRetroviralTagsT2A (from Thosea asigna virus)ExpressionMammalianAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only