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pcDNA-Ran-T24N-mRFP1-polyA

Plasmid

#104561

Purpose

Express Ran T24N mutant in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

Depositor

Yi Zhang

Article

Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

Insert

Ran (Ran Mouse)

Tags

mRFP1

Expression

Mammalian

Mutation

T24N

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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linker-mCitrine-PIF6-NAT

Plasmid

#51576

Purpose

Tag protein of interest with PIF6 in Saccharomyces cerevisiae

Depositor

Chao Tang

Article

Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

Insert

PIF6 (PIL2 Mustard Weed)

Tags

linker and mCitrine

Expression

Yeast

Mutation

1-100aa of PIF6

Promoter

none

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pUltra-U6-gRNAs1-5

Plasmid

#178569

Purpose

Constitutive expression of a gRNA-tRNA array for macsGESTALT editing

Depositor

Christopher Lengner

Article

Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

Insert

5 gRNA-tRNA array with U6 promoter

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-Con/Foff 2.0-BFP

Plasmid

#137130

Purpose

Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

Depositor

Karl Deisseroth, INTRSECT 2.0 Project

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Has Service

AAV8

Insert

Con/Foff 2.0-BFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Mutation

n/a

Promoter

EF1a

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-mTurquoise2

Plasmid

#99125

Purpose

An AAV genome that expresses the fluorescent protein mTurquoise2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pESF140_HisSpy_DDB1∆B

Plasmid

#124213

Purpose

Insect cell expression vector for His6, Spy tagged hsDDB1∆B

Depositor

Eric Fischer

Article

Nowak et al Nat Chem Biol. 2018 Jul;14(7):706-714. d

Insert

DNA damage-binding protein 1 (DDB1 Human)

Tags

His6-Spy

Expression

Insect

Mutation

internal deletion of BPB domain to aid crystalliz…

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mTurquoise2

Plasmid

#99113

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mTurquoise2

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pHelper_AcCAST

Plasmid

#127923

Purpose

Expresses AcCAST and a crRNA targeting PSP1

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

AcTnsB, AcTnsC, AcTniQ, AcCas12k

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-Fos

Plasmid

#131595

Purpose

expression of mouse Fos in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Fos (Fos Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mNeonGreen

Plasmid

#99131

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mNeonGreen

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-145

Plasmid

#118529

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn5 delivery vector

Expression

Bacterial

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-132

Plasmid

#118550

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pJM31

Plasmid

#52986

Purpose

A secreted Annexin V (sAnxV::GFP) Phosphatidylserine sensor

Depositor

Ding Xue

Article

Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

Insert

Annexin V (ANXA5 Human)

Tags

GFP (w/ introns) and signal sequence

Expression

Worm

Promoter

hsp-16.41

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pET28-SaCas9

Plasmid

#178901

Purpose

SaCas9 (Cas9 from Staphylococcus aureus)

Depositor

Hendrik Milting

Article

Flottmann et al STAR Protoc. 2022 Apr 5;3(2):101276. doi

Insert

SaCas9

Tags

His6-Tag, bipartite nuclear localization signal f…

Expression

Bacterial

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pXT131_TwinStrep-SUMO-ShTniQ

Plasmid

#135527

Purpose

Protein expression construct for ShTniQ

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTniQ

Tags

TwinStrep-SUMO

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pXT129_TwinStrep-SUMO-ShTnsB

Plasmid

#135525

Purpose

Protein expression construct for ShTnsB

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsB

Tags

TwinStrep-SUMO

Expression

Bacterial

Available Since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEF_dCas9

Plasmid

#68416

Purpose

Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

dCas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A/H841A

Promoter

human EF1[alpha]

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pTNFR1-tdEOS

Plasmid

#98273

Purpose

mammalian expression of TNFR1-tdEOS

Depositor

Mike Heilemann

Article

Heidbreder et al Biochim Biophys Acta. 2012 Oct;1823(10):

Insert

TNFR1 (TNFRSF1A Human)

Tags

Flag and tdEos

Expression

Mammalian

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-135

Plasmid

#118553

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-155

Plasmid

#118569

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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