We narrowed to 11,682 results for: nts

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2Bc-T MBP_BoMoC(ed)_F753A_6xH

Plasmid

#185710

Purpose

Recombinant B. mori truncated R2 RT expression

Depositor

Kathleen Collins

Article

Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

Insert

B. mori truncated R2 RT

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Changed F753 to A

Promoter

T7

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57-mCherry-2A-BRD4 Iso A

Plasmid

#137720

Purpose

Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform

Depositor

Scott Floyd

Article

Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

Insert

BRD4 long isoform (BRD4 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

Plasmid

#175523

Purpose

Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag11 and Tomm20-HaloTag9

Expression

Mammalian

Mutation

HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLL7 lenti-dcas9-3xflag-blast

Plasmid

#112133

Purpose

lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

dCas9(D10A, N863A)-T2A-Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A mutants in Cas9

Promoter

EF1a

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

Plasmid

#89873

Purpose

expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

bArrestin2-TevC-P2A-TdTomato

Use

AAV

Tags

TdTomato

Expression

Mammalian

Mutation

R173H in TdTomato (Please see depositor comments …

Promoter

Human synapsin

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCMVd1-NLS-GAL4-iLight-VP16-T2A-mTagBFP2

Plasmid

#170273

Purpose

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2 construct expression in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

Insert

NLS-Gal4(1-65)-iLight(human codon optimized)-VP16-T2A-mTagBFP2

Expression

Mammalian

Mutation

See Comments

Promoter

CMVd1

Available Since

June 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJT258 Lenti pEF-dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

Plasmid

#187334

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-N+F+Z-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-v2 RsTAL At4CL

Plasmid

#126524

Purpose

expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

Depositor

Andrew Woolley

Article

Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

Inserts

tyrosine ammonia lyase
p-coumaroyl-CoA ligase (4CL1 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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MigR1-AEtr

Plasmid

#12432

Depositor

Dong-Er Zhang

Article

Yan et al Proc Natl Acad Sci U S A. 2004 Dec 7. 10

Insert

C-term truncated AML1-ETO (RUNX1, RUNX1T1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only
pANC98c(3xFLAG-NES-FRB-(G4S)4-cTEV(219)-IRES-EBFP)

Plasmid

#248359

Purpose

Lentiviral vector that can express FRB along with a truncated C terminal split TEV. Expressed off of CMV tet ON promoter with EBFP marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

3xFLAG-NES-FRB-(G4S)4x-cTEV(219)

Use

Lentiviral

Promoter

tight TRE

Available Since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

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pANC79b(P3-Myc-nTEV-IRES-EBFP)

Plasmid

#248357

Purpose

Lentiviral vector that can express P3 along with N terminal split TEV. Expressed off of CMV tet ON promoter with EBFP marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

P3-Myc-nTEV

Use

Lentiviral

Promoter

tight TRE

Available Since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

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pANC79a(P4-GGGSGGG-cTEV-HA-IRES-dTomato)

Plasmid

#248356

Purpose

Lentiviral vector that can express P4 along with C terminal split TEV. Expressed off of CMV tet ON promoter with dTomato marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

P4-GGGSGGG-cTEV-HA

Use

Lentiviral

Promoter

tight TRE

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pANC98a(3xFLAG-NES-FKBP12-(G4S)4-nTEV-IRES-dTomato)

Plasmid

#248358

Purpose

Lentiviral vector that can express FKBP12 along with N terminal split TEV. Expressed off of CMV tet ON promoter with dTomato marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

3xFLAG-NES-FKBP12-(G4S)4x-nTEV

Use

Lentiviral

Promoter

tight TRE

Available Since

Dec. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pANC61(FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-FKBP12degron-IRES-EGFP)

Plasmid

#248354

Purpose

Lentiviral vector that can express hAqp1_degron based sensor for TEVp in mammalian cells. Expressed off of EF1a promoter with EGFP marker

Depositor

Arnab Mukherjee

Article

Chacko et al Sci Adv. 2026 Jan 16;12(3):eaec1211. doi

Insert

FLAG7 hAqp1-GSG-(TEVcs-GSG)3x-FKBP12degron

Use

Lentiviral

Tags

3x TEV site, FKBP12 degron and FLAG (internal in …

Promoter

EF-1a

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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RcP9_AraC_S170_LOV_P190_Sil_90bp

Plasmid

#242158

Purpose

Recombineering Plasmid 9. Positive control to test for successful editing using AraC S170 LOV (silent mutation of P190, 90bp retron)

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

Gene III

Expression

Bacterial

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pYO759

Plasmid

#235758

Purpose

Expression of FL ScVPS38-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Tags

3xFLAG

Expression

Yeast

Promoter

ScVPS38 promoter

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pDest_PANCEd_AraC_S170_LOV_sd2_WT

Plasmid

#242151

Purpose

Expression Plasmid containing a LacI repressed sd2 WT AraC cassette with a LOV domain insertion at S170

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC S170 LOV

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pYO979

Plasmid

#235756

Purpose

Protein expression of ScVPS38, untagged + ScVPS30 FRK to DDD at 430-432, untagged

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS38 (VPS38 Budding Yeast)
VPS30 (VPS30 Budding Yeast)

Expression

Yeast

Mutation

FRK430-432DDD

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA63_sgRNA-B0_M13ps

Plasmid

#231421

Purpose

M13 phagemid constitutively expressing sgRNA-B0. sgRNA-B0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ552.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-B0

Use

Synthetic Biology

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO411

Plasmid

#235732

Purpose

Protein expression of ZZ-FL yeast Atg14 in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

ATG14

Tags

3xTEV-ZZ

Expression

Yeast

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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