We narrowed to 1,249 results for: vent

1. pCMV5b HA Irx5 deltaIrot

   Plasmid

   #24995

   Depositor

   Benoit Bruneau

   Article

   Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

   Insert

   Irx5 (Irx5 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deletion of Iro box (aa 314-327), 19 aa upstream …

   Promoter

   Available since

   Aug. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pCMV5b HA Irx5 deltaC

   Plasmid

   #24996

   Depositor

   Benoit Bruneau

   Article

   Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

   Insert

   Irx5 (Irx5 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deletes everything after Hox Domain (aa 112-177).…

   Promoter

   Available since

   Aug. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pRRLHygro-pEF1a-p53ashL344A-mKate2-splitmVenusC

   Plasmid

   #69585

   Purpose

   Expresses mutated p53-L344A tagged with mKate2 and split C-term mVenus

   Depositor

   Galit Lahav

   Article

   Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

   Insert

   p53-L344A (TP53 Human)
   

   Use

   Lentiviral

   Tags

   mKate2 and split mVenus - C term

   Expression

   Mammalian

   Mutation

   p53 L344A mutation that prevents tetramerization …

   Promoter

   EF1alpha

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-flex-taCasp3-TEVp

   Plasmid

   #45580

   Depositor

   Nirao Shah, Jim Wells

   Article

   Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

   Has Service

   AAV1 and AAV5

   Inserts

   Caspase 3 (CASP3 Synthetic, Human)
   Tobacco Etch Virus protease
   

   Use

   AAV; Adeno associated viral vector

   Tags

   Expression

   Mutation

   Linker replaced with a TEV protease cleavage site

   Promoter

   EF1a

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry

   Plasmid

   #173030

   Purpose

   To express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cells

   Depositor

   Mohamed Fareh

   Article

   Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

   Insert

   SARS-CoV-2 Nucleocapsid phosphoprotein
   

   Use

   Lentiviral

   Tags

   3xHA and IRES-mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   Gag (truncated)

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-N-Flag-NLRP3 1-90

   Plasmid

   #75137

   Purpose

   Expresses N-terminal Flag-tagged amino acids 1-90 of NLRP3 (the pyrin domain) in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   fragment encoding amino acids 1-90

   Promoter

   CMV

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. FUW-hSNCA-GFP

   Plasmid

   #215489

   Purpose

   Lentiviral expression and easy visualization of alpha-synuclein in mammalian cells

   Depositor

   Ophir Shalem

   Article

   Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

   Insert

   alpha-synuclein (SNCA Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Ubc

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-N-Flag-ASC1

   Plasmid

   #75134

   Purpose

   Expresses N-terminal Flag-tagged ASC1 in mammalian cells

   Depositor

   Bruce Beutler

   Article

   Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

   Insert

   PYD and CARD domain containing (Pycard Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   F23S (please see depositor comments below)

   Promoter

   CMV

   Available since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1-iSAM

   Plasmid

   #211495

   Purpose

   AAVS1 targeting vector containing an all-in-one DOX-inducible system for CRISPRa

   Depositor

   Antonella Fidanza, Lesley Forrester

   Article

   Petazzi et al Elife. 2024 Dec 23;13:RP94884. doi: 10.7

   Inserts

   UniSAM insert
   TET-ON
   Neo Resistance
   Insulator
   

   Use

   CRISPR and Synthetic Biology

   Tags

   T2A-mCherry Tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a, NA, Splicing acceptor, and TREGV promoter

   Available since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

    Plasmid

    #173029

    Purpose

    For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

    Depositor

    Mohamed Fareh

    Article

    Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

    Insert

    pspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, BFP, HIV NES, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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