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Plasmid#216516PurposeExpresses MBP-tagged RNA binding domain of hnRNPAB X2DepositorInserthnRNPAB X2-RBD
UseTags6xHis tags-MBPExpressionBacterialMutationdeleted amino acids 2-64 and 226-304PromoterAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEM112B
Plasmid#210602PurposeReporter gene expression in B. dendrobatidisDepositorInsertSpH2Ater-mClover3-SpH2Apro | SpH2Bpro-GP2A-GT2A-Hshph-Synter8
UseTagsmClover3ExpressionBacterial and YeastMutationPromoterSpH2A/Bpro (divergent)Available sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGO186N-KS1
Plasmid#174301PurposeReplicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.DepositorInsertspacer expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGO186N-KS2
Plasmid#174302PurposeReplicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.DepositorInsertspacer expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQmod3S-GG
Plasmid#191349PurposeClostridium expression vector (pCB102 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
UseTagsExpressionBacterialMutationPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGO186N-KS3
Plasmid#174303PurposeReplicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.DepositorInsertspacer expression cassette
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR8_TaNAM-A1
Plasmid#124574PurposeTaNAM-A1 CDS in the Gateway-compatible pCR8 vector.DepositorInsertTaNAM-A1
UseTopo gateway cloning vectorTagsExpressionMutationPromoterNoneAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ATG-3xHA-S11
Plasmid#108238PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (3xHA-S11 as control) in plantsDepositorInsertATG-3xHA-S11
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
GPD-roGFP
Plasmid#49436PurposeExpresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cellsDepositorInsertGPD-roGFP2
UseAdenoviralTagsGlycerol phosphate dehydrogenase, 5' to roGF…ExpressionMammalianMutationC48S, Q80R, S147C, and Q204C in Clontech's G…PromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28 His6-mEGFP-D4H
Plasmid#226373PurposeProduction of recombinant mEGFP-D4H, a fluorescent probe binding cholesterolDepositorInsertD4H (pfoA Clostridium perfringens)
UseTagsHis6 and monomeric EGFPExpressionBacterialMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDuBir-Lbu-dCas13a-avitag
Plasmid#100817PurposeDual expression of Lbu-dCas13a with a C-terminal avi-tag and BirA to biotinylate Lbu-dCas13aDepositorInsertLbu_Cas13a_R472A_H477A_R1048A_H1053A
UseTagsAviTag and His6-MBPExpressionBacterialMutationR472A, H477A, R1048A, H1053APromoterT7Available sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.1
Plasmid#111053PurposeExpresses dLight1.1 in mammalian cellsDepositorInsertdLight1.1
UseTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.3b
Plasmid#111056PurposeExpresses dLight1.3b in mammalian cellsDepositorInsertdLight1.3b
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.2
Plasmid#111054PurposeExpresses dLight1.2 in mammalian cellsDepositorInsertdLight1.2
UseTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBA-mKeima-PIM
Plasmid#174650PurposeLow level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.DepositorInsertxXFKBP homo-mKeima-2x FKBP homo-2x FKBP
UseTagsExpressionMammalianMutationVal24Glu, Tyr80Cys, Ala95Thr mutations in second …PromoterChicken beta-actinAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-RdLight1
Plasmid#125707PurposeExpresses the red genetically encoded dopamine sensor RdLight1 in mammalian cells.DepositorHas ServiceAAV9InsertdLight1.3b
UseAAVTagsFlag tagExpressionMammalianMutationPromoterCAGAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-RdLight1
Plasmid#125708PurposeExpresses the red genetically encoded dopamine sensor RdLight1 in mammalian neurons.DepositorHas ServiceAAV9InsertRdLight1
UseAAVTagsFlag tagExpressionMutationPromoterhSynapsin1Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ntn1-Fc-His
Plasmid#72104PurposeExpresses the entire Netrin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertNtn1 (Ntn1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pC1-SypHer3s-IMS
Plasmid#108120PurposeMitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.DepositorInsertSypHer3s-IMS
UseTagsratiometricYFPExpressionMammalianMutationPromoterCMVAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry-Cenexin
Plasmid#186435PurposeExpresses mCherry Cenexin in mammalian cells and zebrafish embryosDepositorInsertCenexin (ODF2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMV IE94Available sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only