We narrowed to 7,344 results for: Lif

1. pUE1-TSP-ORF68 R14A/K310A

   Plasmid

   #162655

   Purpose

   Expresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Amino acids R14 and K310 mutated to alanine

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLJM1-zeo-2xStrep-ORF68 C52A

   Plasmid

   #162660

   Purpose

   Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Lentiviral

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Amino acid C52 mutated to alanine

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A

   Plasmid

   #162639

   Purpose

   Expresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cells

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II

   Expression

   Mammalian

   Mutation

   Amino acids K174, R179, and K182 mutated to alani…

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUE1-TSP-ORF68 K435A

   Plasmid

   #162651

   Purpose

   Expresses ORF68 K435A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Amino acid K435 mutated to alanine

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUE1-TSP-ORF68 K450A/R451A

   Plasmid

   #162653

   Purpose

   Expresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Amino acids K450 and R451 mutated to alanine

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA-DAB1

   Plasmid

   #162691

   Purpose

   Expresses DabAB1 inorganic carbon transport complex

   Depositor

   David Savage

   Article

   Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

   Insert

   DabAB1 inorganic carbon transport complex
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLtet0-1 promoter

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. HJP-178-IVS-Syn21-CreAB-NrdJ-1N-WPREw

   Plasmid

   #162473

   Purpose

   This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

   Depositor

   Benjamin White

   Article

   Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

   Insert

   CreAB
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HJP-208-IVS-Syn21-CreAB-NrdJ-1N-WPREUw

   Plasmid

   #162478

   Purpose

   This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

   Depositor

   Benjamin White

   Article

   Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

   Insert

   CreAB
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. HJP-195-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREUw

   Plasmid

   #162476

   Purpose

   This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

   Depositor

   Benjamin White

   Article

   Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

   Insert

   CreB
   

   Use

   Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HJP-194-IVS-Syn21-CreA-gp41-1N-WPREUw

    Plasmid

    #162475

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreA
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HJP-179-IVS-Syn21-NrdJ-1C-CreC-WPREw

    Plasmid

    #162472

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreC
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HJP-176-IVS-Syn21-CreA-gp41-1N-WPREw

    Plasmid

    #162470

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreA
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HJP-196-IVS-Syn21-NrdJ-1C-CreC-WPREUw

    Plasmid

    #162477

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreC
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HJP-180-IVS-Syn21-gp41-1C-CreB-NrdJ-1N-WPREw

    Plasmid

    #162471

    Purpose

    This insert, when expressed in the same cell with other split Cre component, forms Cre recombinase through protein trans-splicing using split intein.

    Depositor

    Benjamin White

    Article

    Luan et al Elife. 2020 Apr 14;9. pii: 53041. doi: 1

    Insert

    CreB
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SMT215

    Plasmid

    #134818

    Purpose

    SMT205 with DHFR-FLAG-tag, western blot

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SMT101

    Plasmid

    #134815

    Purpose

    Dual expression of DHFR and TYMS, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SMT201

    Plasmid

    #134816

    Purpose

    SMT101 with TET promoter for TYMS, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGI3EM11

    Plasmid

    #135483

    Purpose

    Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunH2Bpr hph-GFP ADH1t
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2Bpr

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGI3EM29

    Plasmid

    #135489

    Purpose

    Agrobacterium T-DNA with Spun H2Bpr driving hph-mCitrine

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr hph-mCitrine
    

    Use

    Tags

    mCitrine

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2A/Bpr (divergent)

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGI3EM30

    Plasmid

    #135490

    Purpose

    Agrobacterium T-DNA with Spun H2Bpr driving hph-mClover3

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    hph SpunH2A/Bpr hph-mClover3
    

    Use

    Tags

    mClover3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2A/Bpr (divergent)

    Available since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge