We narrowed to 2,685 results for: buc

1. pGEMHE-mH33B-mClover3

   Plasmid

   #171496

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged mouse H3.3B mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   H3.3B (H3f3b Mouse)
   

   Use

   Pgehme

   Tags

   mClover3

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQCX-CD28 WT

   Plasmid

   #223640

   Purpose

   Retroviral expression of mouse CD28 in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD28 (Cd28 Mouse)
   

   Use

   Retroviral; mammalian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1/MPL-V5

   Plasmid

   #204531

   Purpose

   Mammalian expression of human MPL-V5

   Depositor

   Norio Komatsu

   Article

   Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

   Insert

   MPL-V5 (MPL Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVS1-NDi-CRISPRd (ABI-dCas9-mCherry)

   Plasmid

   #216531

   Purpose

   Targeting vector for CRISPRd generation

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   ABI-dCas9-mCherry
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

   Plasmid

   #181745

   Purpose

   dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   human/zebrafish codon optimized SpCas9
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB PUb-integrase

   Plasmid

   #183966

   Purpose

   piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

   Insert

   PhiC31 integrase
   

   Use

   Piggybac insect transgenesis destination vector

   Tags

   Expression

   Mutation

   Promoter

   Aedes aegypti Polyubiquitin

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEMHE-mClover3-KASH5-DN

   Plasmid

   #171492

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged bovine Kash5 DN mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   KASH5-DN (CCDC155 B. tarus (bovine))
   

   Use

   Pgehme

   Tags

   mClover3

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMHE-GST-KASH5-DN

   Plasmid

   #171493

   Purpose

   T7 promotor drives in vitro transcription of GST-tagged bovine Kash5 DN mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   KASH5-DN (CCDC155 B. tarus (bovine))
   

   Use

   Pgehme

   Tags

   GST

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. Human a3 nicotinic receptor subunit EM construct

   Plasmid

   #163960

   Purpose

   pEZT-BM vector with alpha3 gene after CMV promoter, published EM construct

   Depositor

   Ryan Hibbs

   Article

   Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do

   Insert

   Human alpha3 nicotinic acetylcholine receptor subunit EM construct
   

   Use

   Baculovirus (bacmam) production and mammalian exp…

   Tags

   apocytochrome b(562)RIL (BRIL)

   Expression

   Mammalian

   Mutation

   Asn348-Ser402 replaced with apocytochrome b(562)R…

   Promoter

   CMV

   Available since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX304_zeo

    Plasmid

    #160092

    Purpose

    Lentiviral vector for mammalian gene expression

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

    Plasmid

    #171587

    Purpose

    Bacterial expression of NLS-RfxCas13d-NLS 6His

    Depositor

    Neville Sanjana

    Article

    Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

    Insert

    MKIEE-NLS-RfxCas13d-NLS-HA-6His
    

    Use

    Tags

    HA-6HIS and MKIEE

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CL20_mEGFP_PAX5_PAX5

    Plasmid

    #205887

    Purpose

    Express mEGFP-tagged fusion protein, PAX5_PAX5 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    PAX5_PAX5 (PAX5 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_eGFP (open)

    Plasmid

    #160094

    Purpose

    Expresses eGFP in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    eGFP
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-BTLA (Y226F, Y243F, Y257F, Y282F)-mGFP

    Plasmid

    #180792

    Purpose

    Expression of human BTLA (Y226F, Y243F, Y257F, Y282F) fused to mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLA (BTLA Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Y226F, Y243F, Y257F, Y282F

    Promoter

    SFFV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SpIMPDH

    Plasmid

    #206980

    Purpose

    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

    Depositor

    Vern Schramm

    Article

    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

    Insert

    SpIMPDH (guaB Streptococcus pyogenes)
    

    Use

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti-TRS-Leader-SARS-CoV-2-EGFP

    Plasmid

    #171585

    Purpose

    Expression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporter

    Depositor

    Neville Sanjana

    Article

    Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

    Insert

    TRS-Leader-SARS-CoV-2-d2eGFP
    

    Use

    Lentiviral

    Tags

    2PEST AND NLS

    Expression

    Mutation

    Promoter

    Available since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX304_zeo_eGFP_V5

    Plasmid

    #160096

    Purpose

    Expresses eGFP in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. myc-hRAD18 dSAP

    Plasmid

    #68830

    Purpose

    Expresses c-myc-tagged human RAD18 deleting SAP domain

    Depositor

    Satoshi Tateishi

    Article

    Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

    Insert

    c-myc tagged human RAD18 deleting SAP domain (RAD18 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

19. myc-hRAD18 dC2

    Plasmid

    #68831

    Purpose

    Expresses c-myc-tagged human RAD18 deleting Polymerase eta binding domain

    Depositor

    Satoshi Tateishi

    Article

    Watanabe et al Nucleic Acids Res. 2009 Apr;37(7):2176-9

    Insert

    c-myc tagged human RAD18 deleting Polymerase eta binding domain (RAD18 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

20. psiCHECK-2-TGFBR2-m3_3'UTR

    Plasmid

    #31883

    Depositor

    Robert Blelloch

    Article

    Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

    Insert

    TGFBR2 mutant 3'UTR (TGFBR2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Third miR-302b, 372 7-mer seed match mutated from…

    Promoter

    SV40

    Available since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only