165,688 results

1. pDONR223_HSP90B1_WT

   Plasmid

   #82130

   Purpose

   Gateway Donor vector containing HSP90B1 , part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, William Hahn, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   HSP90B1 (HSP90B1 Human)
   

   Use

   Gateway entry vector

   Mutation

   WT

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEBTet_NADP-Snifit-cyto

   Plasmid

   #113917

   Purpose

   Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NADP-Snifit
   

   Expression

   Mammalian

   Promoter

   CMV TetO2

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pepper-fused sgRNA

   Plasmid

   #217520

   Purpose

   Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

   Depositor

   Xing Li

   Article

   Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

   Insert

   Pepper-fused sgRNA for spCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_256

   Plasmid

   #158581

   Purpose

   Lentiviral expression of BE3.9max and customizable gRNA

   Depositor

   David Root, John Doench

   Article

   Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Cas9 D10A

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKSI-1

   Plasmid

   #51725

   Purpose

   cloning backbone

   Depositor

   Sheng Yang

   Article

   Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

   Type

   Empty backbone

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRSETA_crGE

   Plasmid

   #172927

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

   Plasmid

   #120564

   Purpose

   Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K

   Promoter

   CMV

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. FIRES-PBCS-CLV3

   Plasmid

   #228697

   Purpose

   Sensor cassette FIRE1, FIRE fluorescent ratiometric sensor

   Depositor

   Carolyn Sangokoya

   Article

   Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

   Insert

   FIRE1 SENSOR
   

   Use

   Synthetic Biology and Unspecified

   Tags

   mClover3

   Expression

   Mammalian

   Promoter

   hpgk

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. FIREC-PBCS-MC2

   Plasmid

   #228698

   Purpose

   Control cassette FIRE1, FIRE fluorescent ratiometric sensor

   Depositor

   Carolyn Sangokoya

   Article

   Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

   Insert

   FIRE1 CONTROL
   

   Use

   Synthetic Biology and Unspecified

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   EF1-alpha

   Available Since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-BRCA1-mCherry

    Plasmid

    #195275

    Purpose

    express mCherry-BRCA1 fusion

    Depositor

    Xiaokun Shu

    Article

    Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

    Insert

    mCherry-BRCA1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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